NPL42_ARATH
ID NPL42_ARATH Reviewed; 413 AA.
AC O82264; Q3E6N3;
DT 30-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 25-MAY-2022, entry version 112.
DE RecName: Full=NPL4-like protein 2;
GN OrderedLocusNames=At2g47970; ORFNames=T9J23.1;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Brover V., Troukhan M., Alexandrov N., Lu Y.-P., Flavell R., Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-104, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
CC -!- FUNCTION: May be part of a complex that binds ubiquitinated proteins
CC and that is necessary for the export of misfolded proteins from the ER
CC to the cytoplasm, where they are degraded by the proteasome.
CC {ECO:0000250}.
CC -!- PATHWAY: Protein degradation; proteasomal ubiquitin-dependent pathway.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=O82264-1; Sequence=Displayed;
CC Name=2;
CC IsoId=O82264-2; Sequence=VSP_018605, VSP_018606;
CC -!- SIMILARITY: Belongs to the NPL4 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AF372952; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=AY089192; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AC005309; AAC63651.1; -; Genomic_DNA.
DR EMBL; AC006072; AAM15128.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC10918.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC10919.1; -; Genomic_DNA.
DR EMBL; AF372952; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AY089192; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; BT025256; ABF19009.1; -; mRNA.
DR PIR; G84921; G84921.
DR RefSeq; NP_566118.1; NM_130364.4. [O82264-1]
DR RefSeq; NP_973714.1; NM_201985.1. [O82264-2]
DR AlphaFoldDB; O82264; -.
DR SMR; O82264; -.
DR BioGRID; 4744; 1.
DR STRING; 3702.AT2G47970.1; -.
DR TCDB; 3.A.16.1.5; the endoplasmic reticular retrotranslocon (er-rt) family.
DR iPTMnet; O82264; -.
DR PaxDb; O82264; -.
DR PRIDE; O82264; -.
DR ProteomicsDB; 250544; -. [O82264-1]
DR EnsemblPlants; AT2G47970.1; AT2G47970.1; AT2G47970. [O82264-1]
DR EnsemblPlants; AT2G47970.2; AT2G47970.2; AT2G47970. [O82264-2]
DR GeneID; 819409; -.
DR Gramene; AT2G47970.1; AT2G47970.1; AT2G47970. [O82264-1]
DR Gramene; AT2G47970.2; AT2G47970.2; AT2G47970. [O82264-2]
DR KEGG; ath:AT2G47970; -.
DR Araport; AT2G47970; -.
DR TAIR; locus:2043443; AT2G47970.
DR eggNOG; KOG2834; Eukaryota.
DR HOGENOM; CLU_052923_0_0_1; -.
DR InParanoid; O82264; -.
DR OMA; ESMANTC; -.
DR PhylomeDB; O82264; -.
DR UniPathway; UPA00144; -.
DR PRO; PR:O82264; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O82264; baseline and differential.
DR Genevisible; O82264; AT.
DR GO; GO:0005829; C:cytosol; HDA:TAIR.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0043130; F:ubiquitin binding; IBA:GO_Central.
DR GO; GO:0031625; F:ubiquitin protein ligase binding; IBA:GO_Central.
DR GO; GO:0043161; P:proteasome-mediated ubiquitin-dependent protein catabolic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR CDD; cd08061; MPN_NPL4; 1.
DR InterPro; IPR037518; MPN.
DR InterPro; IPR016563; Npl4.
DR InterPro; IPR007717; NPL4_C.
DR InterPro; IPR024682; Npl4_Ub-like_dom.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR PANTHER; PTHR12710; PTHR12710; 2.
DR Pfam; PF05021; NPL4; 1.
DR Pfam; PF11543; UN_NPL4; 1.
DR SUPFAM; SSF54236; SSF54236; 1.
DR PROSITE; PS50249; MPN; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Phosphoprotein; Reference proteome;
KW Ubl conjugation pathway.
FT CHAIN 1..413
FT /note="NPL4-like protein 2"
FT /id="PRO_0000238459"
FT DOMAIN 131..272
FT /note="MPN"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01182"
FT MOD_RES 104
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19376835"
FT VAR_SEQ 330..354
FT /note="GPLSSTFPIENRSSRATMRALKTHL -> VRVFLSTLPLHQSSLFFVLMLCL
FT GK (in isoform 2)"
FT /evidence="ECO:0000303|Ref.5"
FT /id="VSP_018605"
FT VAR_SEQ 355..413
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.5"
FT /id="VSP_018606"
SQ SEQUENCE 413 AA; 46170 MW; 67CB7F34439C0AB1 CRC64;
MMMLRIRSRD GLERVTAEGA HITVSQLKTL IADQLQIPLH KQTLSTNRDL LLAKTPADLL
AFTDLTDPNL PLSSLNLGHG SMLYLAYDGE RSIPGAPPVT PAGSFGRKMT VDDLIARQMR
VTRQETSHCD SVSFDRDAAN AFQHYVNESL AFAVKRGGFM YGTVTEEGQV EVDFIYEPPQ
QGTEANLILM RDADEEKRVD AIAMGLGMRR VGFIFNQTVV QDKTEYTLSN AEVLQAAELH
AESELKEWVT AVVKLEVNED GGADVHFEAF QMSDMCIRLF KEEWFETEIM PDDDPKLSKM
KKEVVVGVKD LKEVDNDFFL VLVRILDHQG PLSSTFPIEN RSSRATMRAL KTHLDRAKSL
PLVKKMSDFH LLLFVAQFLD VSSDVPALAE CVRLQSPVPE GYALLIESMA NTC
