ID   NPL4_AJECN              Reviewed;         642 AA.
AC   A6R538;
DT   10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT   21-AUG-2007, sequence version 1.
DT   25-MAY-2022, entry version 65.
DE   RecName: Full=Nuclear protein localization protein 4;
GN   Name=NPL4; ORFNames=HCAG_04746;
OS   Ajellomyces capsulatus (strain NAm1 / WU24) (Darling's disease fungus)
OS   (Histoplasma capsulatum).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Onygenales; Ajellomycetaceae; Histoplasma;
OC   unclassified Histoplasma.
OX   NCBI_TaxID=2059318;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NAm1 / WU24;
RX   PubMed=19717792; DOI=10.1101/gr.087551.108;
RA   Sharpton T.J., Stajich J.E., Rounsley S.D., Gardner M.J., Wortman J.R.,
RA   Jordar V.S., Maiti R., Kodira C.D., Neafsey D.E., Zeng Q., Hung C.-Y.,
RA   McMahan C., Muszewska A., Grynberg M., Mandel M.A., Kellner E.M.,
RA   Barker B.M., Galgiani J.N., Orbach M.J., Kirkland T.N., Cole G.T.,
RA   Henn M.R., Birren B.W., Taylor J.W.;
RT   "Comparative genomic analyses of the human fungal pathogens Coccidioides
RT   and their relatives.";
RL   Genome Res. 19:1722-1731(2009).
CC   -!- FUNCTION: Involved in the import of nuclear-targeted proteins into the
CC       nucleus and the export of poly(A) RNA out of the nucleus. Has a role in
CC       the endoplasmic reticulum-associated degradation (ERAD) pathway (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, perinuclear region {ECO:0000250}.
CC       Endoplasmic reticulum membrane {ECO:0000250}; Peripheral membrane
CC       protein {ECO:0000250}; Cytoplasmic side {ECO:0000250}. Nucleus membrane
CC       {ECO:0000250}; Peripheral membrane protein {ECO:0000250}; Cytoplasmic
CC       side {ECO:0000250}. Note=Localizes mainly at the nuclear periphery and
CC       the endoplasmic reticulum membrane. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the NPL4 family. {ECO:0000305}.
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DR   EMBL; CH476658; EDN08236.1; -; Genomic_DNA.
DR   RefSeq; XP_001540906.1; XM_001540856.1.
DR   AlphaFoldDB; A6R538; -.
DR   SMR; A6R538; -.
DR   STRING; 339724.A6R538; -.
DR   EnsemblFungi; EDN08236; EDN08236; HCAG_04746.
DR   GeneID; 5447382; -.
DR   KEGG; aje:HCAG_04746; -.
DR   VEuPathDB; FungiDB:HCAG_04746; -.
DR   HOGENOM; CLU_017172_0_0_1; -.
DR   OMA; KWSRTGR; -.
DR   OrthoDB; 1106766at2759; -.
DR   Proteomes; UP000009297; Unassembled WGS sequence.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro.
DR   CDD; cd08061; MPN_NPL4; 1.
DR   InterPro; IPR037518; MPN.
DR   InterPro; IPR016563; Npl4.
DR   InterPro; IPR007717; NPL4_C.
DR   InterPro; IPR007716; NPL4_Zn-bd_put.
DR   InterPro; IPR029071; Ubiquitin-like_domsf.
DR   PANTHER; PTHR12710; PTHR12710; 1.
DR   Pfam; PF05021; NPL4; 1.
DR   Pfam; PF05020; zf-NPL4; 1.
DR   PIRSF; PIRSF010052; Polyub_prc_Npl4; 1.
DR   SUPFAM; SSF54236; SSF54236; 1.
DR   PROSITE; PS50249; MPN; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Endoplasmic reticulum; Membrane; mRNA transport; Nucleus;
KW   Protein transport; Reference proteome; Translocation; Transport.
FT   CHAIN           1..642
FT                   /note="Nuclear protein localization protein 4"
FT                   /id="PRO_0000339431"
FT   DOMAIN          270..418
FT                   /note="MPN"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01182"
FT   REGION          95..140
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          592..642
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        95..111
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        126..140
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        598..615
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        616..631
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   642 AA;  71205 MW;  152C6F9DA75798C8 CRC64;
     MAPARPILLR FESRNGQFRL TVNPTDEFPS LLPKVLDNLP KNTAPPSIVL SNKPIGTGGQ
     ERNISTLKGV TIQRVGLSHG DKLFIGYEEE TAVVNGSSSE HPSSISKSQN APRRLDGVAV
     RQQEQAPPVP TPTSETLIKN PWEAVKQSPL DDRLDRKDGK ISRGLDHKMC RHGPKGMCDY
     CMPLEPYAPE YLAEKKIKHL SFHSYLRKIN SSTNKPELKS SYIPPLSEPD YRVKRDCPSG
     HPAWPEGICT KCQPSAITLQ PQPFRMVDHV EFSSPDLINS LLDFWRKSGT QRLGFLYGTY
     EEYTEVPLGI KAVVQAIYEP PQVGEVDGVT LHEWGNEKDV DEVAKFCGLE KIGVIFTDLL
     DAGAGDEIVF AAQLQARYPK ATKWSETGRF GSNFVTCVLS GDEDGAISIS AYQASNSAVE
     MVKADIIEPS ADPGVMLVQQ ENHSDDDASK ARYIPEVFYR KVNEYGANVQ ENAKPSFPVE
     YLLVTLTHGF PTDPDVMFNN STFPIENREV IGESQDLRML ADKLVSHGDP NKTICGVSDF
     HLLCFLNSLG ILNKDEEFLL CTVARSHDTA DGMQLINTSG WATLVTILQE SGERPPKRSW
     PFQSQTFISS HQRSKHPQAP KSEHPQSDSE QLAKRFKGAS LG