ID   NPL4_ASHGO              Reviewed;         563 AA.
AC   Q74ZJ1;
DT   10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 102.
DE   RecName: Full=Nuclear protein localization protein 4;
GN   Name=NPL4; OrderedLocusNames=AGR211W;
OS   Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS   (Yeast) (Eremothecium gossypii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX   NCBI_TaxID=284811;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=15001715; DOI=10.1126/science.1095781;
RA   Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA   Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA   Gaffney T.D., Philippsen P.;
RT   "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT   cerevisiae genome.";
RL   Science 304:304-307(2004).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=23749448; DOI=10.1534/g3.112.002881;
RA   Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT   "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT   loci, numerous translocations, lack of transposons, and distinct gene
RT   duplications.";
RL   G3 (Bethesda) 3:1225-1239(2013).
CC   -!- FUNCTION: Involved in the import of nuclear-targeted proteins into the
CC       nucleus and the export of poly(A) RNA out of the nucleus. Has a role in
CC       the endoplasmic reticulum-associated degradation (ERAD) pathway (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, perinuclear region {ECO:0000250}.
CC       Endoplasmic reticulum membrane {ECO:0000250}; Peripheral membrane
CC       protein {ECO:0000250}; Cytoplasmic side {ECO:0000250}. Nucleus membrane
CC       {ECO:0000250}; Peripheral membrane protein {ECO:0000250}; Cytoplasmic
CC       side {ECO:0000250}. Note=Localizes mainly at the nuclear periphery and
CC       the endoplasmic reticulum membrane. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the NPL4 family. {ECO:0000305}.
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DR   EMBL; AE016820; AAS54701.1; -; Genomic_DNA.
DR   RefSeq; NP_986877.1; NM_211939.1.
DR   AlphaFoldDB; Q74ZJ1; -.
DR   SMR; Q74ZJ1; -.
DR   STRING; 33169.AAS54701; -.
DR   PRIDE; Q74ZJ1; -.
DR   EnsemblFungi; AAS54701; AAS54701; AGOS_AGR211W.
DR   GeneID; 4623179; -.
DR   KEGG; ago:AGOS_AGR211W; -.
DR   eggNOG; KOG2834; Eukaryota.
DR   HOGENOM; CLU_017172_0_0_1; -.
DR   InParanoid; Q74ZJ1; -.
DR   OMA; KWSRTGR; -.
DR   Proteomes; UP000000591; Chromosome VII.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0043130; F:ubiquitin binding; IBA:GO_Central.
DR   GO; GO:0031625; F:ubiquitin protein ligase binding; IBA:GO_Central.
DR   GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR   CDD; cd08061; MPN_NPL4; 1.
DR   InterPro; IPR037518; MPN.
DR   InterPro; IPR016563; Npl4.
DR   InterPro; IPR007717; NPL4_C.
DR   InterPro; IPR024682; Npl4_Ub-like_dom.
DR   InterPro; IPR007716; NPL4_Zn-bd_put.
DR   PANTHER; PTHR12710; PTHR12710; 1.
DR   Pfam; PF05021; NPL4; 1.
DR   Pfam; PF11543; UN_NPL4; 1.
DR   Pfam; PF05020; zf-NPL4; 1.
DR   PIRSF; PIRSF010052; Polyub_prc_Npl4; 1.
DR   PROSITE; PS50249; MPN; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Endoplasmic reticulum; Membrane; mRNA transport; Nucleus;
KW   Protein transport; Reference proteome; Translocation; Transport.
FT   CHAIN           1..563
FT                   /note="Nuclear protein localization protein 4"
FT                   /id="PRO_0000339432"
FT   DOMAIN          219..359
FT                   /note="MPN"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01182"
SQ   SEQUENCE   563 AA;  63253 MW;  591379968EC7003D CRC64;
     MILRFRSKHG MQRVNCEGSE QFGTVLDRWV KLVHERAPRE AMEVGVAERQ IETRIAAEMA
     QKTVEQLGLK HGDMLSVSFK ETGGSLAVAA APERSSELAV DRELAREEGL IRRSHSRLCR
     HGDRGMCEYC SPLPPWDRGY QQEQNLKHIS FHAHVKELNE HTNKKASGST YIPPLSPPDF
     HVNKHCPAPH EPWPRGICSK CQPSAISLQQ QEFRMVDHVE FQHSELVNEF INTWRSTGMQ
     RFGYLYGRYA RYDNTPLGIK AVVEAIWEPE QHDEQDGLTM DTVAVRVSVA AVDAIAADMG
     LMRLGMIFTD LTDSGSGDGS VFCKRHKDSF FLSSLEVITA ARHQLHHRNA CRFSDQGFYS
     SKFVTCVVSG NLQGEIDVAA YQVSTDAEAL VDADIISGST HPSMAYINEP SAERYVPEIF
     YMRRNEYGIT IKENAKPAFP VDYLIVSLTH GFPVAADTPP TFQAHTGFPW ANRQAMGQSQ
     DYLELRRVLL PSIAAGDYTE LHRRLSSFHL LLYLHSLQIL DASEWRRLVT VATTPAPRGV
     EAVYDLISGP GWQTLVMILQ EAA