NPL4_ASPNC
ID NPL4_ASPNC Reviewed; 654 AA.
AC A2Q8R9;
DT 10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT 06-MAR-2007, sequence version 1.
DT 25-MAY-2022, entry version 74.
DE RecName: Full=Nuclear protein localization protein 4;
GN Name=npl4; ORFNames=An01g05330;
OS Aspergillus niger (strain CBS 513.88 / FGSC A1513).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Circumdati.
OX NCBI_TaxID=425011;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CBS 513.88 / FGSC A1513 / ATCC MYA-4892;
RX PubMed=17259976; DOI=10.1038/nbt1282;
RA Pel H.J., de Winde J.H., Archer D.B., Dyer P.S., Hofmann G., Schaap P.J.,
RA Turner G., de Vries R.P., Albang R., Albermann K., Andersen M.R.,
RA Bendtsen J.D., Benen J.A.E., van den Berg M., Breestraat S., Caddick M.X.,
RA Contreras R., Cornell M., Coutinho P.M., Danchin E.G.J., Debets A.J.M.,
RA Dekker P., van Dijck P.W.M., van Dijk A., Dijkhuizen L., Driessen A.J.M.,
RA d'Enfert C., Geysens S., Goosen C., Groot G.S.P., de Groot P.W.J.,
RA Guillemette T., Henrissat B., Herweijer M., van den Hombergh J.P.T.W.,
RA van den Hondel C.A.M.J.J., van der Heijden R.T.J.M., van der Kaaij R.M.,
RA Klis F.M., Kools H.J., Kubicek C.P., van Kuyk P.A., Lauber J., Lu X.,
RA van der Maarel M.J.E.C., Meulenberg R., Menke H., Mortimer M.A.,
RA Nielsen J., Oliver S.G., Olsthoorn M., Pal K., van Peij N.N.M.E.,
RA Ram A.F.J., Rinas U., Roubos J.A., Sagt C.M.J., Schmoll M., Sun J.,
RA Ussery D., Varga J., Vervecken W., van de Vondervoort P.J.J., Wedler H.,
RA Woesten H.A.B., Zeng A.-P., van Ooyen A.J.J., Visser J., Stam H.;
RT "Genome sequencing and analysis of the versatile cell factory Aspergillus
RT niger CBS 513.88.";
RL Nat. Biotechnol. 25:221-231(2007).
CC -!- FUNCTION: Involved in the import of nuclear-targeted proteins into the
CC nucleus and the export of poly(A) RNA out of the nucleus. Has a role in
CC the endoplasmic reticulum-associated degradation (ERAD) pathway (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, perinuclear region {ECO:0000250}.
CC Endoplasmic reticulum membrane {ECO:0000250}; Peripheral membrane
CC protein {ECO:0000250}; Cytoplasmic side {ECO:0000250}. Nucleus membrane
CC {ECO:0000250}; Peripheral membrane protein {ECO:0000250}; Cytoplasmic
CC side {ECO:0000250}. Note=Localizes mainly at the nuclear periphery and
CC the endoplasmic reticulum membrane. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the NPL4 family. {ECO:0000305}.
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DR EMBL; AM269966; CAK43702.1; -; Genomic_DNA.
DR AlphaFoldDB; A2Q8R9; -.
DR SMR; A2Q8R9; -.
DR PaxDb; A2Q8R9; -.
DR EnsemblFungi; CAK43702; CAK43702; An01g05330.
DR HOGENOM; CLU_017172_0_0_1; -.
DR Proteomes; UP000006706; Chromosome 2R.
DR GO; GO:0036266; C:Cdc48p-Npl4p-Vms1p AAA ATPase complex; IEA:EnsemblFungi.
DR GO; GO:0000837; C:Doa10p ubiquitin ligase complex; IEA:EnsemblFungi.
DR GO; GO:0000839; C:Hrd1p ubiquitin ligase ERAD-L complex; IEA:EnsemblFungi.
DR GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0030894; C:replisome; IEA:EnsemblFungi.
DR GO; GO:1990112; C:RQC complex; IEA:EnsemblFungi.
DR GO; GO:0034098; C:VCP-NPL4-UFD1 AAA ATPase complex; IEA:EnsemblFungi.
DR GO; GO:0071629; P:cytoplasm protein quality control by the ubiquitin-proteasome system; IEA:EnsemblFungi.
DR GO; GO:0006274; P:DNA replication termination; IEA:EnsemblFungi.
DR GO; GO:0071712; P:ER-associated misfolded protein catabolic process; IEA:EnsemblFungi.
DR GO; GO:0072671; P:mitochondria-associated ubiquitin-dependent protein catabolic process; IEA:EnsemblFungi.
DR GO; GO:0051228; P:mitotic spindle disassembly; IEA:EnsemblFungi.
DR GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
DR GO; GO:0051974; P:negative regulation of telomerase activity; IEA:EnsemblFungi.
DR GO; GO:0070651; P:nonfunctional rRNA decay; IEA:EnsemblFungi.
DR GO; GO:1900182; P:positive regulation of protein localization to nucleus; IEA:EnsemblFungi.
DR GO; GO:0072665; P:protein localization to vacuole; IEA:EnsemblFungi.
DR GO; GO:0030970; P:retrograde protein transport, ER to cytosol; IEA:EnsemblFungi.
DR GO; GO:1990116; P:ribosome-associated ubiquitin-dependent protein catabolic process; IEA:EnsemblFungi.
DR GO; GO:0030433; P:ubiquitin-dependent ERAD pathway; IEA:EnsemblFungi.
DR CDD; cd08061; MPN_NPL4; 1.
DR InterPro; IPR037518; MPN.
DR InterPro; IPR016563; Npl4.
DR InterPro; IPR007717; NPL4_C.
DR InterPro; IPR007716; NPL4_Zn-bd_put.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR PANTHER; PTHR12710; PTHR12710; 1.
DR Pfam; PF05021; NPL4; 1.
DR Pfam; PF05020; zf-NPL4; 1.
DR PIRSF; PIRSF010052; Polyub_prc_Npl4; 1.
DR SUPFAM; SSF54236; SSF54236; 1.
DR PROSITE; PS50249; MPN; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Endoplasmic reticulum; Membrane; mRNA transport; Nucleus;
KW Protein transport; Reference proteome; Translocation; Transport.
FT CHAIN 1..654
FT /note="Nuclear protein localization protein 4"
FT /id="PRO_0000339435"
FT DOMAIN 257..394
FT /note="MPN"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01182"
FT REGION 592..654
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 599..617
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 619..634
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 635..654
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 654 AA; 72790 MW; 9601103E145A892E CRC64;
MASRPIVLRF ESRNGQFRLT VNPQDLFPSL QPQILERLPP NVDPSSITLS NRPIGTGGEE
RLLNSLDGVG FDKVGLKHGD KLFIGYQENQ GQQNGTANGH ITPSAGDASR RLNGAPVPQS
ETATIKNPWD VVQQSPLDDM LDKKDGKIKR GLDTKFCRHG PKGMCDYCMP LEPYDPKYLA
EKKIKHLSFH SYLRKINAST NKAELNSSFM PPLSEPYYRV RRDCPSGHPQ WPEGICTKCQ
PSAISLQPQE FRMVDHVEFS SPDLINSLLD FWRKSGAQRL GFLYGTYEEY KEVPLGVKAV
VQAIYEPPQV DEVDGVTLHE WPNEKEVDEV AHLCGLERIG VIFTDLLDAG RGDGSVICKR
HIDSYYLSSL EIAFAARMQA QHPKATKWSR TGRFGSNFVT CVLSGDEEGA ISVSAYQASV
AAVEMVRADI VEPSAEPSVM LVQSEDDDTE NKSRYIPEVF YRKINEYGVS AQQNAKPSFP
VEYLLVTLTH GFPTDASPLF TDSSFPIENR EVIGESQELV HVAKKLVTHG DPDKAIQAVS
DFHMLCFLHS LSTFNKDEEA LLCRVATQRA PADGLQLINT PGWATLVTIL QESGERPPKR
PWLPTPEPYP PRSVPYNPGK RSLPSSSSSS HLRPESPKSE SERLAKRFKG ASLE
