ID   NPL4_COCIM              Reviewed;         657 AA.
AC   Q1DY54; J3KEP2;
DT   10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT   11-JUL-2006, sequence version 1.
DT   25-MAY-2022, entry version 74.
DE   RecName: Full=Nuclear protein localization protein 4;
GN   Name=NPL4; ORFNames=CIMG_04759;
OS   Coccidioides immitis (strain RS) (Valley fever fungus).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Onygenales; Onygenaceae; Coccidioides.
OX   NCBI_TaxID=246410;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=RS;
RX   PubMed=19717792; DOI=10.1101/gr.087551.108;
RA   Sharpton T.J., Stajich J.E., Rounsley S.D., Gardner M.J., Wortman J.R.,
RA   Jordar V.S., Maiti R., Kodira C.D., Neafsey D.E., Zeng Q., Hung C.-Y.,
RA   McMahan C., Muszewska A., Grynberg M., Mandel M.A., Kellner E.M.,
RA   Barker B.M., Galgiani J.N., Orbach M.J., Kirkland T.N., Cole G.T.,
RA   Henn M.R., Birren B.W., Taylor J.W.;
RT   "Comparative genomic analyses of the human fungal pathogens Coccidioides
RT   and their relatives.";
RL   Genome Res. 19:1722-1731(2009).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=RS;
RX   PubMed=20516208; DOI=10.1101/gr.103911.109;
RA   Neafsey D.E., Barker B.M., Sharpton T.J., Stajich J.E., Park D.J.,
RA   Whiston E., Hung C.-Y., McMahan C., White J., Sykes S., Heiman D.,
RA   Young S., Zeng Q., Abouelleil A., Aftuck L., Bessette D., Brown A.,
RA   FitzGerald M., Lui A., Macdonald J.P., Priest M., Orbach M.J.,
RA   Galgiani J.N., Kirkland T.N., Cole G.T., Birren B.W., Henn M.R.,
RA   Taylor J.W., Rounsley S.D.;
RT   "Population genomic sequencing of Coccidioides fungi reveals recent
RT   hybridization and transposon control.";
RL   Genome Res. 20:938-946(2010).
CC   -!- FUNCTION: Involved in the import of nuclear-targeted proteins into the
CC       nucleus and the export of poly(A) RNA out of the nucleus. Has a role in
CC       the endoplasmic reticulum-associated degradation (ERAD) pathway (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, perinuclear region {ECO:0000250}.
CC       Endoplasmic reticulum membrane {ECO:0000250}; Peripheral membrane
CC       protein {ECO:0000250}; Cytoplasmic side {ECO:0000250}. Nucleus membrane
CC       {ECO:0000250}; Peripheral membrane protein {ECO:0000250}; Cytoplasmic
CC       side {ECO:0000250}. Note=Localizes mainly at the nuclear periphery and
CC       the endoplasmic reticulum membrane. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the NPL4 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=EAS33735.3; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; GG704914; EAS33735.3; ALT_SEQ; Genomic_DNA.
DR   RefSeq; XP_001245318.2; XM_001245317.2.
DR   AlphaFoldDB; Q1DY54; -.
DR   SMR; Q1DY54; -.
DR   STRING; 246410.Q1DY54; -.
DR   EnsemblFungi; EAS33735; EAS33735; CIMG_04759.
DR   GeneID; 4564637; -.
DR   KEGG; cim:CIMG_04759; -.
DR   InParanoid; Q1DY54; -.
DR   OrthoDB; 1106766at2759; -.
DR   Proteomes; UP000001261; Unassembled WGS sequence.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro.
DR   CDD; cd08061; MPN_NPL4; 1.
DR   InterPro; IPR037518; MPN.
DR   InterPro; IPR016563; Npl4.
DR   InterPro; IPR007717; NPL4_C.
DR   InterPro; IPR007716; NPL4_Zn-bd_put.
DR   InterPro; IPR029071; Ubiquitin-like_domsf.
DR   PANTHER; PTHR12710; PTHR12710; 1.
DR   Pfam; PF05021; NPL4; 1.
DR   Pfam; PF05020; zf-NPL4; 1.
DR   PIRSF; PIRSF010052; Polyub_prc_Npl4; 1.
DR   SUPFAM; SSF54236; SSF54236; 1.
DR   PROSITE; PS50249; MPN; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Endoplasmic reticulum; Membrane; mRNA transport; Nucleus;
KW   Protein transport; Reference proteome; Translocation; Transport.
FT   CHAIN           1..657
FT                   /note="Nuclear protein localization protein 4"
FT                   /id="PRO_0000339442"
FT   DOMAIN          268..405
FT                   /note="MPN"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01182"
FT   REGION          116..138
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          608..641
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        616..641
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   657 AA;  72939 MW;  FDB721C52718557F CRC64;
     MAPPKPLILR FESRNGQFRL TVEPAELFPA LLPKILSNLP QNVDPTSIAL SNQPIGTGGQ
     ERKLSLLDGV SIQQVGLKHG DKLFIGYSEQ STLVNGKSSN TSAGNMQHIS RRLNGAPVPE
     QDLPAPPQPS SPTALIKNPW EVVQQSALDD RLDKKDGKIP RGRDLKMCRH GPKGMCDYCM
     PLEPYAPEYL ADKKIKHLSF HSYLRKVNSS KNKPELGSSF MPPLTEPYYR VRKDCPSGHP
     AWPEGICTKC QPSAITLQPQ EFRMVDHVEF ASPDLINSLL DFWRKSGSQR LGFLYGTYEE
     YSEVPLGVKA VVQAIYEPPQ VDEVDGITLR EWENERDVDE VAKLCGLEKV GVIFTDLLDS
     GLGDGTVICR RHIDSYYLSS LEVAFAARLQ ARYPKPSKWS ETGRFGSNFV TCILSGDENG
     AISISAYQAS NSAVEMVRAD IVEPSADPSV MLVQRENELE NADTGNARYI PEVFYRKVNE
     YGANVQENAK PAFPVEYLFV TLTHGFPTEP SALFADTTFP IENREVIGES QDIRNVAKKL
     LSRSDPDEAI RAVSNFHLLC FMHGLGILSK EEEALLCTVA RTHDPADGVQ LINTPGWATL
     VTILQESGEP PPKRSWPFQT ESSSSNKNHT RHVPLYPKTL TSDSDQLAKR FKGASLE