NPL4_CRYNJ
ID NPL4_CRYNJ Reviewed; 693 AA.
AC P0CP30; Q55VJ9; Q5KKN9;
DT 28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 25-MAY-2022, entry version 46.
DE RecName: Full=Nuclear protein localization protein 4;
GN Name=NPL4; OrderedLocusNames=CNC02370;
OS Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC
OS MYA-565) (Filobasidiella neoformans).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC Tremellales; Cryptococcaceae; Cryptococcus;
OC Cryptococcus neoformans species complex.
OX NCBI_TaxID=214684;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JEC21 / ATCC MYA-565;
RX PubMed=15653466; DOI=10.1126/science.1103773;
RA Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D.,
RA Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E.,
RA Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A.,
RA Fox D.S., Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C.,
RA Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J.,
RA Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A.,
RA Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E.,
RA Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A.,
RA Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W.,
RA Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W.;
RT "The genome of the basidiomycetous yeast and human pathogen Cryptococcus
RT neoformans.";
RL Science 307:1321-1324(2005).
CC -!- FUNCTION: Involved in the import of nuclear-targeted proteins into the
CC nucleus and the export of poly(A) RNA out of the nucleus. Has a role in
CC the endoplasmic reticulum-associated degradation (ERAD) pathway (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, perinuclear region {ECO:0000250}.
CC Endoplasmic reticulum membrane {ECO:0000250}; Peripheral membrane
CC protein {ECO:0000250}; Cytoplasmic side {ECO:0000250}. Nucleus membrane
CC {ECO:0000250}; Peripheral membrane protein {ECO:0000250}; Cytoplasmic
CC side {ECO:0000250}. Note=Localizes mainly at the nuclear periphery and
CC the endoplasmic reticulum membrane. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the NPL4 family. {ECO:0000305}.
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DR EMBL; AE017343; AAW42211.1; -; Genomic_DNA.
DR RefSeq; XP_569518.1; XM_569518.1.
DR AlphaFoldDB; P0CP30; -.
DR SMR; P0CP30; -.
DR STRING; 5207.AAW42211; -.
DR PaxDb; P0CP30; -.
DR EnsemblFungi; AAW42211; AAW42211; CNC02370.
DR GeneID; 3256137; -.
DR KEGG; cne:CNC02370; -.
DR VEuPathDB; FungiDB:CNC02370; -.
DR eggNOG; KOG2834; Eukaryota.
DR HOGENOM; CLU_017172_1_0_1; -.
DR InParanoid; P0CP30; -.
DR OMA; KWSRTGR; -.
DR OrthoDB; 1106766at2759; -.
DR Proteomes; UP000002149; Chromosome 3.
DR GO; GO:0036266; C:Cdc48p-Npl4p-Vms1p AAA ATPase complex; IEA:EnsemblFungi.
DR GO; GO:0000837; C:Doa10p ubiquitin ligase complex; IEA:EnsemblFungi.
DR GO; GO:0000839; C:Hrd1p ubiquitin ligase ERAD-L complex; IEA:EnsemblFungi.
DR GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0030894; C:replisome; IEA:EnsemblFungi.
DR GO; GO:1990112; C:RQC complex; IEA:EnsemblFungi.
DR GO; GO:0034098; C:VCP-NPL4-UFD1 AAA ATPase complex; IEA:EnsemblFungi.
DR GO; GO:0043130; F:ubiquitin binding; IBA:GO_Central.
DR GO; GO:0031625; F:ubiquitin protein ligase binding; IBA:GO_Central.
DR GO; GO:0071629; P:cytoplasm protein quality control by the ubiquitin-proteasome system; IEA:EnsemblFungi.
DR GO; GO:0006274; P:DNA replication termination; IEA:EnsemblFungi.
DR GO; GO:0071712; P:ER-associated misfolded protein catabolic process; IEA:EnsemblFungi.
DR GO; GO:0072671; P:mitochondria-associated ubiquitin-dependent protein catabolic process; IEA:EnsemblFungi.
DR GO; GO:0051228; P:mitotic spindle disassembly; IEA:EnsemblFungi.
DR GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
DR GO; GO:0051974; P:negative regulation of telomerase activity; IEA:EnsemblFungi.
DR GO; GO:0070651; P:nonfunctional rRNA decay; IEA:EnsemblFungi.
DR GO; GO:1900182; P:positive regulation of protein localization to nucleus; IEA:EnsemblFungi.
DR GO; GO:0072665; P:protein localization to vacuole; IEA:EnsemblFungi.
DR GO; GO:0030970; P:retrograde protein transport, ER to cytosol; IEA:EnsemblFungi.
DR GO; GO:1990116; P:ribosome-associated ubiquitin-dependent protein catabolic process; IEA:EnsemblFungi.
DR GO; GO:0030433; P:ubiquitin-dependent ERAD pathway; IEA:EnsemblFungi.
DR GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR CDD; cd08061; MPN_NPL4; 1.
DR InterPro; IPR037518; MPN.
DR InterPro; IPR016563; Npl4.
DR InterPro; IPR007717; NPL4_C.
DR InterPro; IPR007716; NPL4_Zn-bd_put.
DR PANTHER; PTHR12710; PTHR12710; 1.
DR Pfam; PF05021; NPL4; 1.
DR Pfam; PF05020; zf-NPL4; 1.
DR PIRSF; PIRSF010052; Polyub_prc_Npl4; 1.
DR PROSITE; PS50249; MPN; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Endoplasmic reticulum; Membrane; mRNA transport; Nucleus;
KW Protein transport; Reference proteome; Translocation; Transport.
FT CHAIN 1..693
FT /note="Nuclear protein localization protein 4"
FT /id="PRO_0000339443"
FT DOMAIN 259..399
FT /note="MPN"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01182"
FT REGION 85..132
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 613..665
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 101..120
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 613..632
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 693 AA; 75103 MW; 5532ECBFEA134A03 CRC64;
MLLRIRSPAG TARLTVQPET TGEDFAEAIL NTIPAADPQP DPATLALSNQ PGAAGESVPF
HALSGRTVGD MGFSHGDLLF LSYKPRAADP DSHPAMQATA PHPQPAQPDP SHPKTHTDPP
MPNTIPLRDL SSVQEPEIDQ YWEKQTGKIE RKRDPAFCRH GDKAMCDYCM PLEPYDPKFQ
SEHQIKHLSY HAYLRKLLSS RPPTASSATD LPPLSPTSLS VITPCPTGAH PSFPDGICST
CQPSAVTLQS QPFRMVDHIE FASPSIIEGL LSAWRRTGTQ RIAFLIGRED KYEKVPMGIK
VIVEAVWEPK QEGELDGLTV ETPWSDESRV QEIAKWCDKG LSVVGMIYTD LTPSPDDITK
TLYKRHAQSY TASSLEMLLS AAYQLSHPLS TRMSPTGHYS SRFVTCCLTG DKDGGVDILA
WQASEHAEAM VKAGIVEASV DPAVVRVRKP GEGEYVPEVF YSYKNEYGLQ VKMPAKPTFP
VEYLYVNITH GFPLAPSPLF LSNAFPTENR PGLHDQSMQV VITQLAAILK TSDAEIGDAG
TWPGRIKKDV EKWLSDWHLV TFLCMQGLFS LKEQQILCRA ATAHAHPNDT HALEELFASG
GWQTLLTIVD SEASANARSN PPPTSSFNNL GIDSPAFAGP STESSAPPSG PDSVGAGAGA
GAGGGRERVC PHCTFVNEHG GSDCEICGLP LDG
