ID   NPL4_DICDI              Reviewed;         576 AA.
AC   Q54GD3;
DT   10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   03-AUG-2022, entry version 85.
DE   RecName: Full=Nuclear protein localization protein 4 homolog;
GN   Name=nploc4; Synonyms=npl4; ORFNames=DDB_G0290227;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: May be part of a complex that binds ubiquitinated proteins
CC       and that is necessary for the export of misfolded proteins from the ER
CC       to the cytoplasm, where they are degraded by the proteasome.
CC       {ECO:0000250}.
CC   -!- PATHWAY: Protein degradation; proteasomal ubiquitin-dependent pathway.
CC   -!- SIMILARITY: Belongs to the NPL4 family. {ECO:0000305}.
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DR   EMBL; AAFI02000161; EAL62335.1; -; Genomic_DNA.
DR   RefSeq; XP_635845.1; XM_630753.1.
DR   AlphaFoldDB; Q54GD3; -.
DR   STRING; 44689.DDB0233722; -.
DR   PaxDb; Q54GD3; -.
DR   EnsemblProtists; EAL62335; EAL62335; DDB_G0290227.
DR   GeneID; 8627553; -.
DR   KEGG; ddi:DDB_G0290227; -.
DR   dictyBase; DDB_G0290227; npl4.
DR   eggNOG; KOG2834; Eukaryota.
DR   HOGENOM; CLU_473653_0_0_1; -.
DR   InParanoid; Q54GD3; -.
DR   OMA; RVGWIFS; -.
DR   PhylomeDB; Q54GD3; -.
DR   Reactome; R-DDI-8951664; Neddylation.
DR   Reactome; R-DDI-9755511; KEAP1-NFE2L2 pathway.
DR   UniPathway; UPA00144; -.
DR   PRO; PR:Q54GD3; -.
DR   Proteomes; UP000002195; Chromosome 5.
DR   GO; GO:0005783; C:endoplasmic reticulum; ISS:dictyBase.
DR   GO; GO:0042175; C:nuclear outer membrane-endoplasmic reticulum membrane network; ISS:dictyBase.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0043130; F:ubiquitin binding; IBA:GO_Central.
DR   GO; GO:0031625; F:ubiquitin protein ligase binding; IBA:GO_Central.
DR   GO; GO:0030433; P:ubiquitin-dependent ERAD pathway; ISS:dictyBase.
DR   GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR   CDD; cd08061; MPN_NPL4; 1.
DR   InterPro; IPR037518; MPN.
DR   InterPro; IPR016563; Npl4.
DR   InterPro; IPR007717; NPL4_C.
DR   InterPro; IPR029071; Ubiquitin-like_domsf.
DR   PANTHER; PTHR12710; PTHR12710; 1.
DR   Pfam; PF05021; NPL4; 2.
DR   SUPFAM; SSF54236; SSF54236; 1.
DR   PROSITE; PS50249; MPN; 1.
PE   3: Inferred from homology;
KW   Reference proteome; Ubl conjugation pathway.
FT   CHAIN           1..576
FT                   /note="Nuclear protein localization protein 4 homolog"
FT                   /id="PRO_0000339458"
FT   DOMAIN          295..430
FT                   /note="MPN"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01182"
FT   REGION          67..96
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          200..219
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        68..96
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   576 AA;  64399 MW;  50A79B9EF98D898F CRC64;
     MIIAVRSPSG TTKINVSDTA IVKDLYVKLK ENNVDIDGKS ITKTPRDPPL EVGDKLSSLG
     IKSGDLLHLV GNNNNNNNDN KASSGSNNNN NNNNISKKEI SYDEKLPNGL TPRCKHAVGD
     KCVFCSDAQG MKRCDHPENA TCPNCQNKKF KGKRLKWLCN HPAGGKCSNC LRVGKNVSYK
     CNHGSTATCV NCMDKDDEDQ DDNEEDNKDN KDNSEIKKSG FVPKVAIKNE VKTKCLHGPN
     QKCINCLPKD DPNSIEVPKR RCKNHGINGS CVECIEWRES LKMRLKSQDN PHAPGALVDF
     QSANIFQQYI ANSKYEQQRI GFLFGNFLSD GSVVVDSIYE PPQECKDKQT PTLLPDPLAD
     KIESMASMLG LTRVGWIFSH PSRKYTMSST EIIQAASYQN KYGPSFVTLI LSVNSDGQSN
     MEAFQVSDQA LKLEKTGEFL PTQPDPTKCK LKSPVFEEGT ETINADTHFF IVTVPLKARE
     DKSIFNISFP VENRIPVNTL SDLASYKLEH KDVSPLKFFS DFHFLIFLLE NQFLDFQSDF
     PIICENIRSR SSENLIGYLE IINYQIGDII GHDQFN