NPL4_MAGO7
ID NPL4_MAGO7 Reviewed; 669 AA.
AC A4RN19; G4MZE9; G4MZF0;
DT 10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT 25-JAN-2012, sequence version 2.
DT 25-MAY-2022, entry version 92.
DE RecName: Full=Nuclear protein localization protein 4;
GN Name=NPL4; ORFNames=MGG_04413;
OS Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast
OS fungus) (Pyricularia oryzae).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Sordariomycetidae; Magnaporthales; Pyriculariaceae; Pyricularia.
OX NCBI_TaxID=242507;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ISOFORMS 1 AND 2).
RC STRAIN=70-15 / ATCC MYA-4617 / FGSC 8958;
RX PubMed=15846337; DOI=10.1038/nature03449;
RA Dean R.A., Talbot N.J., Ebbole D.J., Farman M.L., Mitchell T.K.,
RA Orbach M.J., Thon M.R., Kulkarni R., Xu J.-R., Pan H., Read N.D.,
RA Lee Y.-H., Carbone I., Brown D., Oh Y.Y., Donofrio N., Jeong J.S.,
RA Soanes D.M., Djonovic S., Kolomiets E., Rehmeyer C., Li W., Harding M.,
RA Kim S., Lebrun M.-H., Bohnert H., Coughlan S., Butler J., Calvo S.E.,
RA Ma L.-J., Nicol R., Purcell S., Nusbaum C., Galagan J.E., Birren B.W.;
RT "The genome sequence of the rice blast fungus Magnaporthe grisea.";
RL Nature 434:980-986(2005).
CC -!- FUNCTION: Involved in the import of nuclear-targeted proteins into the
CC nucleus and the export of poly(A) RNA out of the nucleus. Has a role in
CC the endoplasmic reticulum-associated degradation (ERAD) pathway (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, perinuclear region {ECO:0000250}.
CC Endoplasmic reticulum membrane {ECO:0000250}; Peripheral membrane
CC protein {ECO:0000250}; Cytoplasmic side {ECO:0000250}. Nucleus membrane
CC {ECO:0000250}; Peripheral membrane protein {ECO:0000250}; Cytoplasmic
CC side {ECO:0000250}. Note=Localizes mainly at the nuclear periphery and
CC the endoplasmic reticulum membrane. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=A4RN19-1; Sequence=Displayed;
CC Name=2;
CC IsoId=A4RN19-2; Sequence=VSP_042194;
CC -!- SIMILARITY: Belongs to the NPL4 family. {ECO:0000305}.
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DR EMBL; CM001232; EHA53704.1; -; Genomic_DNA.
DR EMBL; CM001232; EHA53705.1; -; Genomic_DNA.
DR RefSeq; XP_003713511.1; XM_003713463.1. [A4RN19-2]
DR RefSeq; XP_003713512.1; XM_003713464.1. [A4RN19-1]
DR AlphaFoldDB; A4RN19; -.
DR SMR; A4RN19; -.
DR STRING; 318829.MGG_04413T0; -.
DR EnsemblFungi; MGG_04413T0; MGG_04413T0; MGG_04413. [A4RN19-1]
DR GeneID; 2677741; -.
DR KEGG; mgr:MGG_04413; -.
DR VEuPathDB; FungiDB:MGG_04413; -.
DR eggNOG; KOG2834; Eukaryota.
DR InParanoid; A4RN19; -.
DR OMA; KWSRTGR; -.
DR OrthoDB; 1106766at2759; -.
DR Proteomes; UP000009058; Chromosome 2.
DR GO; GO:0036266; C:Cdc48p-Npl4p-Vms1p AAA ATPase complex; IEA:EnsemblFungi.
DR GO; GO:0005829; C:cytosol; ISS:PAMGO_MGG.
DR GO; GO:0000837; C:Doa10p ubiquitin ligase complex; IEA:EnsemblFungi.
DR GO; GO:0005783; C:endoplasmic reticulum; ISS:PAMGO_MGG.
DR GO; GO:0000839; C:Hrd1p ubiquitin ligase ERAD-L complex; IEA:EnsemblFungi.
DR GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0042175; C:nuclear outer membrane-endoplasmic reticulum membrane network; ISS:PAMGO_MGG.
DR GO; GO:0005634; C:nucleus; ISS:PAMGO_MGG.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0030894; C:replisome; IEA:EnsemblFungi.
DR GO; GO:1990112; C:RQC complex; IEA:EnsemblFungi.
DR GO; GO:0034098; C:VCP-NPL4-UFD1 AAA ATPase complex; IEA:EnsemblFungi.
DR GO; GO:0071629; P:cytoplasm protein quality control by the ubiquitin-proteasome system; IEA:EnsemblFungi.
DR GO; GO:0006274; P:DNA replication termination; IEA:EnsemblFungi.
DR GO; GO:0071712; P:ER-associated misfolded protein catabolic process; IEA:EnsemblFungi.
DR GO; GO:0072671; P:mitochondria-associated ubiquitin-dependent protein catabolic process; IEA:EnsemblFungi.
DR GO; GO:0051228; P:mitotic spindle disassembly; IEA:EnsemblFungi.
DR GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
DR GO; GO:0051974; P:negative regulation of telomerase activity; IEA:EnsemblFungi.
DR GO; GO:0070651; P:nonfunctional rRNA decay; IEA:EnsemblFungi.
DR GO; GO:1900182; P:positive regulation of protein localization to nucleus; IEA:EnsemblFungi.
DR GO; GO:0072665; P:protein localization to vacuole; IEA:EnsemblFungi.
DR GO; GO:0030970; P:retrograde protein transport, ER to cytosol; IEA:EnsemblFungi.
DR GO; GO:1990116; P:ribosome-associated ubiquitin-dependent protein catabolic process; IEA:EnsemblFungi.
DR GO; GO:0030433; P:ubiquitin-dependent ERAD pathway; ISS:PAMGO_MGG.
DR GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; ISS:PAMGO_MGG.
DR CDD; cd08061; MPN_NPL4; 1.
DR InterPro; IPR037518; MPN.
DR InterPro; IPR016563; Npl4.
DR InterPro; IPR007717; NPL4_C.
DR InterPro; IPR007716; NPL4_Zn-bd_put.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR PANTHER; PTHR12710; PTHR12710; 1.
DR Pfam; PF05021; NPL4; 1.
DR Pfam; PF05020; zf-NPL4; 1.
DR PIRSF; PIRSF010052; Polyub_prc_Npl4; 1.
DR SUPFAM; SSF54236; SSF54236; 1.
DR PROSITE; PS50249; MPN; 1.
PE 3: Inferred from homology;
KW Alternative splicing; Cytoplasm; Endoplasmic reticulum; Membrane;
KW mRNA transport; Nucleus; Protein transport; Reference proteome;
KW Translocation; Transport.
FT CHAIN 1..669
FT /note="Nuclear protein localization protein 4"
FT /id="PRO_0000339448"
FT DOMAIN 263..400
FT /note="MPN"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01182"
FT REGION 87..112
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 613..637
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 90..111
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 608..669
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_042194"
SQ SEQUENCE 669 AA; 74006 MW; 38A512A08960B2EF CRC64;
MLLRLRGPDG MLRIELDPKD TFNKLGQELM GKLPPTVDPA TITVSNAPGS QGDKKLLKDI
AKYKVEAIGL KHGDLIFVDY KHQGAEADGT ANSDGASQPL TSTTNRLNGQ PVLPTEDLPI
DPLPTPAPGA TIKNPWEVVR QSPLDDRLDK KDGKIPRKRD AMCRHGPKGM CDYCQPLDPF
DAKFLAEKKI KYLSMHAHLR KINSATNKPE LGSSFIPPLS EPYFRVKHDC PSGHPQWPEG
ICSKCQPSAI TLQPQPFRMV DHVEFASPSI VDSFINTWRR TGGQRYGIMY GKYSEYEEVP
LGIKAVVQAI YEPPQVDEVD GVSLNSWDNE KDVNQVARLC GLEPVGAIWT DLLDAGAGDG
SVVCKRHADS YFLSSLEVCF AARLQAQHPK PSKWSDTGRF GSNFVTCIIS GNEQGEIAIS
SYQVSNEAVE MVRADIMEPS ADPTVMLVRE EEEDDGSTSR TRYIPDVFYR RINEYGANVQ
ENAKPSFPVE YLFVTLTHGF PDVAKPMFSD EGAFPIENRE YMGESQEHSA AAKALKVHEK
ASSGSSKDGM KVSNFHLLCF LHQMSVLSKD EESLLCRVAT QHDLADAFQL RSTTGWQTLM
AILQSTGERI PKRSRQTDVL AADPAASSYP GRRGIDDSDE RLAKRFASVR LNARGDGRNG
RDRPSAHET
