ID   AROE_THESQ              Reviewed;         253 AA.
AC   B1L9E4;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   29-APR-2008, sequence version 1.
DT   03-AUG-2022, entry version 75.
DE   RecName: Full=Shikimate dehydrogenase;
DE            EC=1.1.1.25;
GN   Name=aroE; OrderedLocusNames=TRQ2_0589;
OS   Thermotoga sp. (strain RQ2).
OC   Bacteria; Thermotogae; Thermotogales; Thermotogaceae; Thermotoga;
OC   unclassified Thermotoga.
OX   NCBI_TaxID=126740;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=RQ2;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E.,
RA   Tice H., Bruce D.B., Goodwin L., Pitluck S., Saunders E., Brettin T.,
RA   Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L.,
RA   Kyrpides N., Mikhailova N., Nelson K., Gogarten J.P., Noll K.,
RA   Richardson P.;
RT   "Complete sequence of Thermotoga sp. RQ2.";
RL   Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=NADP(+) + shikimate = 3-dehydroshikimate + H(+) + NADPH;
CC         Xref=Rhea:RHEA:17737, ChEBI:CHEBI:15378, ChEBI:CHEBI:16630,
CC         ChEBI:CHEBI:36208, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.1.1.25;
CC   -!- PATHWAY: Metabolic intermediate biosynthesis; chorismate biosynthesis;
CC       chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step
CC       4/7.
CC   -!- SIMILARITY: Belongs to the shikimate dehydrogenase family.
CC       {ECO:0000305}.
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DR   EMBL; CP000969; ACB08942.1; -; Genomic_DNA.
DR   RefSeq; WP_011943195.1; NC_010483.1.
DR   AlphaFoldDB; B1L9E4; -.
DR   SMR; B1L9E4; -.
DR   EnsemblBacteria; ACB08942; ACB08942; TRQ2_0589.
DR   KEGG; trq:TRQ2_0589; -.
DR   HOGENOM; CLU_044063_4_1_0; -.
DR   OMA; SIFARND; -.
DR   OrthoDB; 1054867at2; -.
DR   UniPathway; UPA00053; UER00087.
DR   Proteomes; UP000001687; Chromosome.
DR   GO; GO:0004764; F:shikimate 3-dehydrogenase (NADP+) activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0009073; P:aromatic amino acid family biosynthetic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0008652; P:cellular amino acid biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0009423; P:chorismate biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR046346; Aminiacid_DH-like_N_sf.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR013708; Shikimate_DH-bd_N.
DR   InterPro; IPR022893; Shikimate_DH_fam.
DR   InterPro; IPR006151; Shikm_DH/Glu-tRNA_Rdtase.
DR   PANTHER; PTHR21089; PTHR21089; 1.
DR   Pfam; PF01488; Shikimate_DH; 1.
DR   Pfam; PF08501; Shikimate_dh_N; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   SUPFAM; SSF53223; SSF53223; 1.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis; Aromatic amino acid biosynthesis; NADP;
KW   Oxidoreductase.
FT   CHAIN           1..253
FT                   /note="Shikimate dehydrogenase"
FT                   /id="PRO_1000100149"
FT   ACT_SITE        63
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255"
FT   BINDING         115..119
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   253 AA;  28874 MW;  A56A15CAD162A3D2 CRC64;
     MKFCIIGYPV SHSISPRLYN EYFKRAGMNH SYGMEEIPPE SFDTEIRRIL EEYDGFNATI
     PHKERVMRYV EPSEDAQRIK AVNCVFRGKG YNTDWVGVVK SLEGVEVKEP VVVVGAGGAA
     RAVIYALLQM GVKDIWVVNR TIERAKALDF PVKIFSLDQL DEVVKKAKSL FNTTSVGMKG
     EKLTVSEASL KGLYLVYDVV YFETPLVSDA KRLGVEHVVK GNLMFYYQAM ENLKIWGIYD
     ERSFKEVFEE VLR