ID   NPL4_NEOFI              Reviewed;         652 AA.
AC   A1D4X8;
DT   10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 1.
DT   25-MAY-2022, entry version 84.
DE   RecName: Full=Nuclear protein localization protein 4;
GN   Name=npl4; ORFNames=NFIA_021800;
OS   Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164
OS   / JCM 1740 / NRRL 181 / WB 181) (Aspergillus fischerianus).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Fumigati.
OX   NCBI_TaxID=331117;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181
RC   / WB 181;
RX   PubMed=18404212; DOI=10.1371/journal.pgen.1000046;
RA   Fedorova N.D., Khaldi N., Joardar V.S., Maiti R., Amedeo P., Anderson M.J.,
RA   Crabtree J., Silva J.C., Badger J.H., Albarraq A., Angiuoli S., Bussey H.,
RA   Bowyer P., Cotty P.J., Dyer P.S., Egan A., Galens K., Fraser-Liggett C.M.,
RA   Haas B.J., Inman J.M., Kent R., Lemieux S., Malavazi I., Orvis J.,
RA   Roemer T., Ronning C.M., Sundaram J.P., Sutton G., Turner G., Venter J.C.,
RA   White O.R., Whitty B.R., Youngman P., Wolfe K.H., Goldman G.H.,
RA   Wortman J.R., Jiang B., Denning D.W., Nierman W.C.;
RT   "Genomic islands in the pathogenic filamentous fungus Aspergillus
RT   fumigatus.";
RL   PLoS Genet. 4:E1000046-E1000046(2008).
CC   -!- FUNCTION: Involved in the import of nuclear-targeted proteins into the
CC       nucleus and the export of poly(A) RNA out of the nucleus. Has a role in
CC       the endoplasmic reticulum-associated degradation (ERAD) pathway (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, perinuclear region {ECO:0000250}.
CC       Endoplasmic reticulum membrane {ECO:0000250}; Peripheral membrane
CC       protein {ECO:0000250}; Cytoplasmic side {ECO:0000250}. Nucleus membrane
CC       {ECO:0000250}; Peripheral membrane protein {ECO:0000250}; Cytoplasmic
CC       side {ECO:0000250}. Note=Localizes mainly at the nuclear periphery and
CC       the endoplasmic reticulum membrane. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the NPL4 family. {ECO:0000305}.
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DR   EMBL; DS027688; EAW23471.1; -; Genomic_DNA.
DR   RefSeq; XP_001265368.1; XM_001265367.1.
DR   AlphaFoldDB; A1D4X8; -.
DR   SMR; A1D4X8; -.
DR   STRING; 36630.CADNFIAP00001975; -.
DR   EnsemblFungi; EAW23471; EAW23471; NFIA_021800.
DR   GeneID; 4590895; -.
DR   KEGG; nfi:NFIA_021800; -.
DR   VEuPathDB; FungiDB:NFIA_021800; -.
DR   eggNOG; KOG2834; Eukaryota.
DR   HOGENOM; CLU_017172_0_0_1; -.
DR   OMA; KWSRTGR; -.
DR   OrthoDB; 1106766at2759; -.
DR   Proteomes; UP000006702; Unassembled WGS sequence.
DR   GO; GO:0036266; C:Cdc48p-Npl4p-Vms1p AAA ATPase complex; IEA:EnsemblFungi.
DR   GO; GO:0000837; C:Doa10p ubiquitin ligase complex; IEA:EnsemblFungi.
DR   GO; GO:0000839; C:Hrd1p ubiquitin ligase ERAD-L complex; IEA:EnsemblFungi.
DR   GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0030894; C:replisome; IEA:EnsemblFungi.
DR   GO; GO:1990112; C:RQC complex; IEA:EnsemblFungi.
DR   GO; GO:0034098; C:VCP-NPL4-UFD1 AAA ATPase complex; IEA:EnsemblFungi.
DR   GO; GO:0071629; P:cytoplasm protein quality control by the ubiquitin-proteasome system; IEA:EnsemblFungi.
DR   GO; GO:0006274; P:DNA replication termination; IEA:EnsemblFungi.
DR   GO; GO:0071712; P:ER-associated misfolded protein catabolic process; IEA:EnsemblFungi.
DR   GO; GO:0072671; P:mitochondria-associated ubiquitin-dependent protein catabolic process; IEA:EnsemblFungi.
DR   GO; GO:0051228; P:mitotic spindle disassembly; IEA:EnsemblFungi.
DR   GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
DR   GO; GO:0051974; P:negative regulation of telomerase activity; IEA:EnsemblFungi.
DR   GO; GO:0070651; P:nonfunctional rRNA decay; IEA:EnsemblFungi.
DR   GO; GO:1900182; P:positive regulation of protein localization to nucleus; IEA:EnsemblFungi.
DR   GO; GO:0072665; P:protein localization to vacuole; IEA:EnsemblFungi.
DR   GO; GO:0030970; P:retrograde protein transport, ER to cytosol; IEA:EnsemblFungi.
DR   GO; GO:1990116; P:ribosome-associated ubiquitin-dependent protein catabolic process; IEA:EnsemblFungi.
DR   GO; GO:0030433; P:ubiquitin-dependent ERAD pathway; IEA:EnsemblFungi.
DR   CDD; cd08061; MPN_NPL4; 1.
DR   InterPro; IPR037518; MPN.
DR   InterPro; IPR016563; Npl4.
DR   InterPro; IPR007717; NPL4_C.
DR   InterPro; IPR007716; NPL4_Zn-bd_put.
DR   InterPro; IPR029071; Ubiquitin-like_domsf.
DR   PANTHER; PTHR12710; PTHR12710; 1.
DR   Pfam; PF05021; NPL4; 1.
DR   Pfam; PF05020; zf-NPL4; 1.
DR   PIRSF; PIRSF010052; Polyub_prc_Npl4; 1.
DR   SUPFAM; SSF54236; SSF54236; 1.
DR   PROSITE; PS50249; MPN; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Endoplasmic reticulum; Membrane; mRNA transport; Nucleus;
KW   Protein transport; Reference proteome; Translocation; Transport.
FT   CHAIN           1..652
FT                   /note="Nuclear protein localization protein 4"
FT                   /id="PRO_0000339449"
FT   DOMAIN          265..402
FT                   /note="MPN"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01182"
FT   REGION          601..652
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        629..645
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   652 AA;  73019 MW;  B9EDC80A3A5CBEF4 CRC64;
     MAAARPIILR FESRNGQFRL SVSPQEQFPL LQEKILENLP KDVEPSSLVL SNKPIGTGGQ
     ERQLKDLAGV SIERVGLKHG DKLFIGYQDK QASQAAPAHK HVTADVSRRL NGAPVPETET
     VTFHPPTSPS ATIKNPWEVV QQSPLDDMLD KKDGKIYRPR DPKMCNHGPK GMCDYCMPLE
     PYDPKYLAEK KIKHLSFHSY MRKVNATTNK AELKSSFMPP LNEPYYRVRR DCPSGHPPWP
     EGICTKCQPS AISLQPQEFR MVDHVEFASP DLINSLLDFW RKSGAQRLGF LYGTYEEYTE
     VPLGIKAVVQ AIYEPPQVDE IDGITLHEWP NEKEVDEVAR QCGLEKVGVI FTDLLDAGRG
     DGSVVCKRHI DSYYLSSLEI AFASRMQAKH PKATKWSRTG RFGSNFVTCV LSGDEEGAIT
     VSSYQASISA VEMVRADIVE PSAEPSVMLV QSEDDDTDNK SRYIPEVFYR KINEYGVSAQ
     QNAKPSFPVE YLLVTLTHGF PTEASPMFSA STFPIENREV IGESQELRHV AKKLVSHGDP
     DKAIREVSDF HLLCFLHSLS MFSKEEEALL CRVATTHDPT EGLKLLNTPG WATLVTVLQE
     SGERPPKRPW LNPADPPRPL SQQGKRHLSS RPESPKSESE QLAKRFKGAS LE