ID   NPL4_PICST              Reviewed;         577 AA.
AC   A3GFS1;
DT   10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT   19-JAN-2010, sequence version 2.
DT   25-MAY-2022, entry version 75.
DE   RecName: Full=Nuclear protein localization protein 4;
GN   Name=NPL4; ORFNames=PICST_80363;
OS   Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL
OS   Y-11545) (Yeast) (Pichia stipitis).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Debaryomycetaceae; Scheffersomyces.
OX   NCBI_TaxID=322104;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545;
RX   PubMed=17334359; DOI=10.1038/nbt1290;
RA   Jeffries T.W., Grigoriev I.V., Grimwood J., Laplaza J.M., Aerts A.,
RA   Salamov A., Schmutz J., Lindquist E., Dehal P., Shapiro H., Jin Y.-S.,
RA   Passoth V., Richardson P.M.;
RT   "Genome sequence of the lignocellulose-bioconverting and xylose-fermenting
RT   yeast Pichia stipitis.";
RL   Nat. Biotechnol. 25:319-326(2007).
CC   -!- FUNCTION: Involved in the import of nuclear-targeted proteins into the
CC       nucleus and the export of poly(A) RNA out of the nucleus. Has a role in
CC       the endoplasmic reticulum-associated degradation (ERAD) pathway (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, perinuclear region {ECO:0000250}.
CC       Endoplasmic reticulum membrane {ECO:0000250}; Peripheral membrane
CC       protein {ECO:0000250}; Cytoplasmic side {ECO:0000250}. Nucleus membrane
CC       {ECO:0000250}; Peripheral membrane protein {ECO:0000250}; Cytoplasmic
CC       side {ECO:0000250}. Note=Localizes mainly at the nuclear periphery and
CC       the endoplasmic reticulum membrane. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the NPL4 family. {ECO:0000305}.
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DR   EMBL; AAVQ01000001; EAZ63804.2; -; Genomic_DNA.
DR   RefSeq; XP_001387827.2; XM_001387790.1.
DR   AlphaFoldDB; A3GFS1; -.
DR   SMR; A3GFS1; -.
DR   STRING; 4924.XP_001387827.2; -.
DR   EnsemblFungi; EAZ63804; EAZ63804; PICST_80363.
DR   GeneID; 4851120; -.
DR   KEGG; pic:PICST_80363; -.
DR   eggNOG; KOG2834; Eukaryota.
DR   HOGENOM; CLU_017172_0_0_1; -.
DR   InParanoid; A3GFS1; -.
DR   OMA; KWSRTGR; -.
DR   OrthoDB; 1106766at2759; -.
DR   Proteomes; UP000002258; Chromosome 1.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro.
DR   CDD; cd08061; MPN_NPL4; 1.
DR   InterPro; IPR037518; MPN.
DR   InterPro; IPR016563; Npl4.
DR   InterPro; IPR007717; NPL4_C.
DR   InterPro; IPR007716; NPL4_Zn-bd_put.
DR   InterPro; IPR029071; Ubiquitin-like_domsf.
DR   PANTHER; PTHR12710; PTHR12710; 1.
DR   Pfam; PF05021; NPL4; 1.
DR   Pfam; PF05020; zf-NPL4; 1.
DR   PIRSF; PIRSF010052; Polyub_prc_Npl4; 1.
DR   SUPFAM; SSF54236; SSF54236; 1.
DR   PROSITE; PS50249; MPN; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Endoplasmic reticulum; Membrane; mRNA transport; Nucleus;
KW   Protein transport; Reference proteome; Translocation; Transport.
FT   CHAIN           1..577
FT                   /note="Nuclear protein localization protein 4"
FT                   /id="PRO_0000339452"
FT   DOMAIN          246..383
FT                   /note="MPN"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01182"
SQ   SEQUENCE   577 AA;  64524 MW;  0EB464364E7FEE76 CRC64;
     MSVTLRFRSR EGTFRVAANP DADFLLVLEQ LLSKISIEDV QNLYLSDKPN SKGELANGLC
     GKTVTELGLK NGDMLYASYE AATGSNPDST TNITTSTNNH NSGSISIGHI SIPTTTSGPR
     KVTQLPVDDV LEKDEGLIKR PLTKFCRHGA KGMCEFCSPL PPWDANYRKE NAIKHMSYHA
     YLKELNELKN SKHNSSSYIA PLEEPNYSIL LNCNEGHQPY PKGICSKCQP PPITLQLQKF
     RMVDHVEFAT SSIMNNFIDV WRHTGVQRFG VMYGRYEPFD KVPLGIKAVV EAIYEPPQSG
     ELDGITMLPW ENEAEVDAIA SELGIYKVGV VFTDLTDSGQ KNGTVLCKRH KDSYFLSNLE
     ILMAARNQIQ HANITKFSSS GQFSSKFVTC VISGGLNGEI EPRSYQVSTS AEALVRADII
     TGSTQPSRLY VNSSNDRRYV PDVAYSELNE YGLEVKSNAK PTFPVDFLLV SLTDSFPVNP
     TPMFDTDSNF VIENRDFFNE LQNLHAVSKY LNADTSGKGT SLCNFHFLVY LKRTNILGAQ
     EFDLLLRFVR ERQYEDYLHL VESPGWMTLI TILEQST