NPLP1_DROME
ID NPLP1_DROME Reviewed; 309 AA.
AC Q9W0W6; Q95T41;
DT 17-JAN-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 2.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Neuropeptide-like 1;
DE Contains:
DE RecName: Full=MTYamide peptide;
DE Contains:
DE RecName: Full=IPNamide peptide;
DE Contains:
DE RecName: Full=NAP peptide;
DE Contains:
DE RecName: Full=NPLP1-1;
DE Contains:
DE RecName: Full=NPLP1-2;
DE Contains:
DE RecName: Full=NPLP1-3;
DE Contains:
DE RecName: Full=NPLP1-4;
DE Contains:
DE RecName: Full=NPLP1-5;
DE Contains:
DE RecName: Full=NPLP1-6;
DE Flags: Precursor;
GN Name=Nplp1; ORFNames=CG3441;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Ovary;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP PROTEIN SEQUENCE OF 102-115; 151-164 AND 168-182, AND AMIDATION AT TYR-164
RP AND ASN-182.
RC TISSUE=Larva;
RX PubMed=12171930; DOI=10.1074/jbc.m206257200;
RA Baggerman G., Cerstiaens A., De Loof A., Schoofs L.;
RT "Peptidomics of the larval Drosophila melanogaster central nervous
RT system.";
RL J. Biol. Chem. 277:40368-40374(2002).
RN [5]
RP PROTEIN SEQUENCE OF 102-115; 151-164 AND 168-182, AND TISSUE SPECIFICITY.
RC TISSUE=CNS;
RX PubMed=14690519; DOI=10.1046/j.1471-4159.2003.02161.x;
RA Verleyen P., Baggerman G., Wiehart U., Schoeters E., Van Lommel A.,
RA De Loof A., Schoofs L.;
RT "Expression of a novel neuropeptide, NVGTLARDFQLPIPNamide, in the larval
RT and adult brain of Drosophila melanogaster.";
RL J. Neurochem. 88:311-319(2004).
RN [6]
RP FUNCTION (NPLP1-4 PEPTIDE).
RX PubMed=21893139; DOI=10.1016/j.peptides.2011.08.019;
RA Overend G., Cabrero P., Guo A.X., Sebastian S., Cundall M., Armstrong H.,
RA Mertens I., Schoofs L., Dow J.A., Davies S.A.;
RT "The receptor guanylate cyclase Gyc76C and a peptide ligand, NPLP1-VQQ,
RT modulate the innate immune IMD pathway in response to salt stress.";
RL Peptides 34:209-218(2012).
RN [7]
RP FUNCTION (NPLP1-4 PEPTIDE), AND DISRUPTION PHENOTYPE (NPLP1-4 PEPTIDE).
RX PubMed=26440503; DOI=10.1371/journal.pgen.1005576;
RA Schleede J., Blair S.S.;
RT "The Gyc76C receptor guanylyl cyclase and the foraging cGMP-dependent
RT kinase regulate extracellular matrix organization and BMP signaling in the
RT developing wing of Drosophila melanogaster.";
RL PLoS Genet. 11:E1005576-E1005576(2015).
CC -!- FUNCTION: [NPLP1-4]: Acts as a ligand for the receptor-type guanylate
CC cyclase Gyc76C (PubMed:21893139). Stimulates Gyc76c-dependent cGMP
CC production and modulates the IMD innate immune pathway in response to
CC salt stress by inducing nuclear translocation of NF-kappa-B protein Rel
CC which leads to increased expression of the antimicrobial peptide
CC diptericin (PubMed:21893139). Does not appear to play a role in Gyc76C-
CC mediated wing development (PubMed:26440503).
CC {ECO:0000269|PubMed:21893139, ECO:0000269|PubMed:26440503}.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: MTYamide peptide: Expressed in the larval CNS (at
CC protein level). NAP peptide: Expressed in the larval CNS (at protein
CC level). IPNamide peptide: Expressed in the ventral ganglion of the
CC third larval instar and adult brain (at protein level).
CC {ECO:0000269|PubMed:14690519}.
CC -!- DISRUPTION PHENOTYPE: [NPLP1-4]: Occasional ectopic branching from the
CC posterior crossvein. {ECO:0000269|PubMed:26440503}.
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DR EMBL; AE013599; AAF47313.2; -; Genomic_DNA.
DR EMBL; AY060340; AAL25379.1; -; mRNA.
DR RefSeq; NP_611993.1; NM_138149.3.
DR AlphaFoldDB; Q9W0W6; -.
DR SMR; Q9W0W6; -.
DR BioGRID; 63571; 4.
DR DIP; DIP-17872N; -.
DR IntAct; Q9W0W6; 1.
DR STRING; 7227.FBpp0288531; -.
DR PaxDb; Q9W0W6; -.
DR PRIDE; Q9W0W6; -.
DR DNASU; 38003; -.
DR EnsemblMetazoa; FBtr0072443; FBpp0072348; FBgn0035092.
DR GeneID; 38003; -.
DR KEGG; dme:Dmel_CG3441; -.
DR CTD; 100529111; -.
DR FlyBase; FBgn0035092; Nplp1.
DR VEuPathDB; VectorBase:FBgn0035092; -.
DR eggNOG; ENOG502S8KI; Eukaryota.
DR InParanoid; Q9W0W6; -.
DR BioGRID-ORCS; 38003; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 38003; -.
DR PRO; PR:Q9W0W6; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0035092; Expressed in brain and 13 other tissues.
DR ExpressionAtlas; Q9W0W6; baseline and differential.
DR Genevisible; Q9W0W6; DM.
DR GO; GO:0005576; C:extracellular region; NAS:UniProtKB.
DR GO; GO:0005615; C:extracellular space; IC:FlyBase.
DR GO; GO:0005184; F:neuropeptide hormone activity; IDA:FlyBase.
DR GO; GO:0048018; F:receptor ligand activity; IPI:FlyBase.
DR GO; GO:0007218; P:neuropeptide signaling pathway; NAS:UniProtKB.
DR GO; GO:0007168; P:receptor guanylyl cyclase signaling pathway; IDA:FlyBase.
PE 1: Evidence at protein level;
KW Amidation; Cleavage on pair of basic residues; Direct protein sequencing;
KW Neuropeptide; Reference proteome; Secreted; Signal.
FT SIGNAL 1..28
FT /evidence="ECO:0000255"
FT PROPEP 29..99
FT /id="PRO_0000021832"
FT PEPTIDE 102..115
FT /note="NAP peptide"
FT /id="PRO_0000021833"
FT PEPTIDE 118..148
FT /note="NPLP1-1"
FT /evidence="ECO:0000255"
FT /id="PRO_0000021834"
FT PEPTIDE 151..164
FT /note="MTYamide peptide"
FT /id="PRO_0000021835"
FT PEPTIDE 168..182
FT /note="IPNamide peptide"
FT /id="PRO_0000021836"
FT PEPTIDE 186..203
FT /note="NPLP1-2"
FT /evidence="ECO:0000255"
FT /id="PRO_0000021837"
FT PEPTIDE 206..226
FT /note="NPLP1-3"
FT /evidence="ECO:0000255"
FT /id="PRO_0000021838"
FT PEPTIDE 229..243
FT /note="NPLP1-4"
FT /evidence="ECO:0000255"
FT /id="PRO_0000021839"
FT PEPTIDE 246..287
FT /note="NPLP1-5"
FT /evidence="ECO:0000255"
FT /id="PRO_0000021840"
FT PEPTIDE 290..309
FT /note="NPLP1-6"
FT /evidence="ECO:0000255"
FT /id="PRO_0000021841"
FT REGION 126..147
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 164
FT /note="Tyrosine amide"
FT /evidence="ECO:0000269|PubMed:12171930"
FT MOD_RES 182
FT /note="Asparagine amide"
FT /evidence="ECO:0000269|PubMed:12171930"
SQ SEQUENCE 309 AA; 34198 MW; 620BB8A464BEF487 CRC64;
MQAVLQSAHS SRRLMLLLSM LLNAAIQPRS IIVSATDDVA NVSPCEMESL INQLMSPSPE
YQLHASALRN QLKNLLRERQ LAVGEEQPLG EYPDYLEEDK RSVAALAAQG LLNAPKRSLA
TLAKNGQLPT AEPGEDYGDA DSGEPSEQKR YIGSLARAGG LMTYGKRNVG TLARDFQLPI
PNGKRNIATM ARLQSAPSTH RDPKRNVAAV ARYNSQHGHI QRAGAEKRNL GALKSSPVHG
VQQKREDEEM LLPAAAPDYA DPMQSYWWYP SYAGYADLDW NDYRRAEKRF LGRVLPPTRA
TASTHRSRL
