NPLP2_DROME
ID NPLP2_DROME Reviewed; 86 AA.
AC Q9VU58;
DT 17-JAN-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Neuropeptide-like 2;
DE AltName: Full=NEF peptide;
DE Flags: Precursor;
GN Name=Nplp2; ORFNames=CG11051;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP PROTEIN SEQUENCE OF 35-44.
RC TISSUE=Larva;
RX PubMed=12171930; DOI=10.1074/jbc.m206257200;
RA Baggerman G., Cerstiaens A., De Loof A., Schoofs L.;
RT "Peptidomics of the larval Drosophila melanogaster central nervous
RT system.";
RL J. Biol. Chem. 277:40368-40374(2002).
RN [5]
RP PROTEIN SEQUENCE OF 35-44.
RC TISSUE=CNS;
RX PubMed=14690519; DOI=10.1046/j.1471-4159.2003.02161.x;
RA Verleyen P., Baggerman G., Wiehart U., Schoeters E., Van Lommel A.,
RA De Loof A., Schoofs L.;
RT "Expression of a novel neuropeptide, NVGTLARDFQLPIPNamide, in the larval
RT and adult brain of Drosophila melanogaster.";
RL J. Neurochem. 88:311-319(2004).
RN [6]
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION BY BACTERIA, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Hemolymph {ECO:0000303|PubMed:16510152};
RX PubMed=16510152; DOI=10.1016/j.jinsphys.2005.12.007;
RA Verleyen P., Baggerman G., D'Hertog W., Vierstraete E., Husson S.J.,
RA Schoofs L.;
RT "Identification of new immune induced molecules in the haemolymph of
RT Drosophila melanogaster by 2D-nanoLC MS/MS.";
RL J. Insect Physiol. 52:379-388(2006).
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:16510152}.
CC -!- TISSUE SPECIFICITY: Hemolymph (at protein level).
CC {ECO:0000269|PubMed:16510152}.
CC -!- INDUCTION: By bacterial infection (at protein level).
CC {ECO:0000269|PubMed:16510152}.
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DR EMBL; AE014296; AAF49832.1; -; Genomic_DNA.
DR EMBL; AY113560; AAM29565.1; -; mRNA.
DR RefSeq; NP_001287059.1; NM_001300130.1.
DR RefSeq; NP_652430.1; NM_144173.3.
DR AlphaFoldDB; Q9VU58; -.
DR SMR; Q9VU58; -.
DR BioGRID; 72623; 28.
DR DIP; DIP-19165N; -.
DR IntAct; Q9VU58; 21.
DR STRING; 7227.FBpp0075575; -.
DR PaxDb; Q9VU58; -.
DR PRIDE; Q9VU58; -.
DR DNASU; 50286; -.
DR EnsemblMetazoa; FBtr0075839; FBpp0075575; FBgn0287423.
DR EnsemblMetazoa; FBtr0346389; FBpp0312079; FBgn0287423.
DR GeneID; 50286; -.
DR KEGG; dme:Dmel_CG11051; -.
DR CTD; 50286; -.
DR FlyBase; FBgn0287423; Nplp2.
DR VEuPathDB; VectorBase:FBgn0287423; -.
DR HOGENOM; CLU_167078_0_0_1; -.
DR InParanoid; Q9VU58; -.
DR PhylomeDB; Q9VU58; -.
DR BioGRID-ORCS; 50286; 0 hits in 1 CRISPR screen.
DR ChiTaRS; Nplp2; fly.
DR GenomeRNAi; 50286; -.
DR PRO; PR:Q9VU58; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR ExpressionAtlas; Q9VU58; baseline and differential.
DR Genevisible; Q9VU58; DM.
DR GO; GO:0005576; C:extracellular region; NAS:UniProtKB.
DR GO; GO:0005615; C:extracellular space; IDA:FlyBase.
DR GO; GO:0005184; F:neuropeptide hormone activity; NAS:UniProtKB.
DR GO; GO:0006959; P:humoral immune response; IEP:FlyBase.
DR GO; GO:0007218; P:neuropeptide signaling pathway; NAS:UniProtKB.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Neuropeptide; Reference proteome; Secreted;
KW Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT PROPEP 20..34
FT /id="PRO_0000021842"
FT PEPTIDE 35..44
FT /note="Neuropeptide-like 2"
FT /id="PRO_0000021843"
FT PROPEP 45..86
FT /id="PRO_0000021844"
SQ SEQUENCE 86 AA; 9412 MW; 67C95A49535B262F CRC64;
MAKLAICILV FALFALALSA RVPREESNPA QEFLTKAQGD FNEFIEKLKA LDAKKVEGLF
KDGLNTVQEG LQKLNETFLQ APAAST
