ID   NPM_LITCT               Reviewed;         198 AA.
AC   Q1HTZ9; C1C3W3;
DT   29-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT   13-JUN-2006, sequence version 1.
DT   25-MAY-2022, entry version 44.
DE   RecName: Full=Nucleoplasmin {ECO:0000305};
GN   Name=np {ECO:0000312|EMBL:ABC69369.1};
OS   Lithobates catesbeianus (American bullfrog) (Rana catesbeiana).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Ranoidea; Ranidae; Lithobates.
OX   NCBI_TaxID=8400 {ECO:0000312|EMBL:ABC69369.1};
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBUNIT, SUBCELLULAR
RP   LOCATION, AND TISSUE SPECIFICITY.
RX   PubMed=16646973; DOI=10.1186/1471-2164-7-99;
RA   Frehlick L.J., Eirin-Lopez J.M., Jeffery E.D., Hunt D.F., Ausio J.;
RT   "The characterization of amphibian nucleoplasmins yields new insight into
RT   their role in sperm chromatin remodeling.";
RL   BMC Genomics 7:99-99(2006).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RA   Helbing C.C., Veldhoen N., Leong J., Koop B.F.;
RT   "Rana catesbeiana ESTs and full-length cDNAs.";
RL   Submitted (APR-2009) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Acts as a chaperone for histones, such as histone H2A-H2B,
CC       and thus regulates the assembly of nucleosome cores. Involved in
CC       chromatin remodeling, especially during fertilization and early
CC       embryonic development. May be involved in sperm chromatin
CC       decondensation during fertilization. {ECO:0000250|UniProtKB:P05221,
CC       ECO:0000250|UniProtKB:Q86SE8}.
CC   -!- SUBUNIT: Homopentamer. {ECO:0000305|PubMed:16646973}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:16646973}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q1HTZ9-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q1HTZ9-2; Sequence=VSP_057076;
CC   -!- TISSUE SPECIFICITY: Mainly expressed in oocytes.
CC       {ECO:0000269|PubMed:16646973}.
CC   -!- SIMILARITY: Belongs to the nucleoplasmin family. {ECO:0000305}.
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DR   EMBL; DQ340656; ABC69369.1; -; mRNA.
DR   EMBL; BT081542; ACO51673.1; -; mRNA.
DR   AlphaFoldDB; Q1HTZ9; -.
DR   SMR; Q1HTZ9; -.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0006325; P:chromatin organization; IEA:UniProtKB-KW.
DR   GO; GO:0007338; P:single fertilization; IEA:UniProtKB-KW.
DR   InterPro; IPR004301; Nucleoplasmin.
DR   InterPro; IPR024057; Nucleoplasmin_core_dom.
DR   InterPro; IPR036824; Nucleoplasmin_core_dom_sf.
DR   PANTHER; PTHR22747; PTHR22747; 1.
DR   Pfam; PF03066; Nucleoplasmin; 1.
DR   SUPFAM; SSF69203; SSF69203; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; Chaperone; Chromatin regulator;
KW   Developmental protein; Fertilization; Nucleus.
FT   CHAIN           1..198
FT                   /note="Nucleoplasmin"
FT                   /id="PRO_0000430716"
FT   REGION          35..38
FT                   /note="Acidic tract A1"
FT                   /evidence="ECO:0000305"
FT   REGION          126..198
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          129..147
FT                   /note="Acidic tract A2"
FT                   /evidence="ECO:0000305"
FT   REGION          174..176
FT                   /note="Acidic tract A3"
FT                   /evidence="ECO:0000305"
FT   MOTIF           154..169
FT                   /note="Bipartite nuclear localization signal"
FT                   /evidence="ECO:0000250|UniProtKB:P05221"
FT   COMPBIAS        126..146
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   SITE            57
FT                   /note="Interaction between pentamers"
FT                   /evidence="ECO:0000250|UniProtKB:P05221"
FT   SITE            83
FT                   /note="Interaction between pentamers"
FT                   /evidence="ECO:0000250|UniProtKB:P05221"
FT   VAR_SEQ         141..173
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|Ref.2"
FT                   /id="VSP_057076"
FT   CONFLICT        81
FT                   /note="T -> A (in Ref. 2; ACO51673)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        131
FT                   /note="E -> G (in Ref. 2; ACO51673)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   198 AA;  21749 MW;  3F3DD95C1F3AD7F9 CRC64;
     MDSTISNTSK LDKPVSLIWG CELNEQTKTF TFKVSDEDKS EHQLALRTVC LGNAAKDEFH
     IVEIVPQAVE GSDAKPVPIA TLKPSILPMA TMVGIELTPP VTFQLKSGSG PIYISGQHVA
     LEADYSWAEE EEGEEEVEEE EEEEDPESPP KPVKRPAASK KAGQAKKKKM EKDEEESSEE
     EDSPAKKGKG RGRKPSKK