NPP5_CAEEL
ID NPP5_CAEEL Reviewed; 813 AA.
AC Q19131;
DT 07-JUN-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Nuclear pore complex protein 5 {ECO:0000312|WormBase:F07A11.3};
DE AltName: Full=Nuclear pore complex protein Nup107 {ECO:0000305};
DE AltName: Full=Nucleoporin npp-5 {ECO:0000305};
GN Name=npp-5 {ECO:0000312|WormBase:F07A11.3};
GN ORFNames=F07A11.3 {ECO:0000312|WormBase:F07A11.3};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP DEVELOPMENTAL STAGE.
RX PubMed=19167330; DOI=10.1016/j.cell.2008.11.037;
RA D'Angelo M.A., Raices M., Panowski S.H., Hetzer M.W.;
RT "Age-dependent deterioration of nuclear pore complexes causes a loss of
RT nuclear integrity in postmitotic cells.";
RL Cell 136:284-295(2009).
RN [3] {ECO:0000305}
RP FUNCTION, INTERACTION WITH MDF-1, SUBCELLULAR LOCATION, DEVELOPMENTAL
RP STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=22238360; DOI=10.1091/mbc.e11-11-0927;
RA Rodenas E., Gonzalez-Aguilera C., Ayuso C., Askjaer P.;
RT "Dissection of the NUP107 nuclear pore subcomplex reveals a novel
RT interaction with spindle assembly checkpoint protein MAD1 in Caenorhabditis
RT elegans.";
RL Mol. Biol. Cell 23:930-944(2012).
CC -!- FUNCTION: Involved in kinetochore assembly and chromosome segregation
CC during embryonic mitosis. Required for the localization of the NDC80
CC complex member him-10, the chromosomal passenger complex component air-
CC 2 and nuclear pore complex proteins npp-23 and npp-15 to kinetochores
CC during metaphase. Required for npp-23 localization to the nuclear
CC envelope during interphase. Recruits mdf-1, a component of the spindle
CC assembly checkpoint, to the nuclear envelope. Appears dispensable for
CC the assembly of the nuclear pore complex and for nuclear protein
CC import. {ECO:0000269|PubMed:22238360}.
CC -!- SUBUNIT: Part of the nuclear pore complex (NPC) (By similarity). May
CC interact with mdf-1 (PubMed:22238360). {ECO:0000250|UniProtKB:P57740,
CC ECO:0000269|PubMed:22238360}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nuclear pore complex
CC {ECO:0000250|UniProtKB:P57740}. Chromosome, centromere, kinetochore
CC {ECO:0000269|PubMed:22238360}. Nucleus membrane
CC {ECO:0000269|PubMed:22238360}; Peripheral membrane protein
CC {ECO:0000305}. Note=Localizes to nuclear membrane periphery during
CC interphase and to kinetochore during mitosis.
CC {ECO:0000269|PubMed:22238360}.
CC -!- DEVELOPMENTAL STAGE: Highly expressed in embryos (at protein level)
CC (PubMed:19167330, PubMed:22238360). Expression decreases rapidly in
CC larvae and becomes restricted to the germline in adults
CC (PubMed:19167330). {ECO:0000269|PubMed:19167330,
CC ECO:0000269|PubMed:22238360}.
CC -!- DISRUPTION PHENOTYPE: Larval lethality with about 9-12 percent of
CC animals reaching adulthood. The few surviving adults are sterile. About
CC 10 percent embryonic lethality. Reduced nuclear growth in P1 embryonic
CC cell and delayed entry into mitosis. 39 percent reduction in him-10
CC kinetochore association and 34 percent reduction in air-2 chromatin
CC association during metaphase. Loss of npp-5 localization to
CC kinetochores during metaphase. About 10 percent of mutants have
CC chromosome segregation defects. RNAi-mediated knockdown causes a loss
CC of npp-23 localization to kinetochores and reduced localization to the
CC nuclear envelope in interphase. Also results in the accumulation of
CC spindle assembly checkpoint protein mdf-1 on kinetochores during
CC metaphase and reduced mdf-1 localization to the nuclear envelope. Does
CC not affect the expression levels of nuclear pore complex components
CC npp-10, npp-19 and npp-7 or npp-10, npp-2 and mel-28 localization and
CC appears to be dispensable for pie-1 nuclear import and for nuclear
CC protein exclusion. Embryos transiently exposed to a hypoxic environment
CC develop into infertile adults. Simultaneous RNAi-mediated knockdown of
CC mdf-1 causes embryonic lethality and severe chromosome segregation
CC defects. {ECO:0000269|PubMed:22238360}.
CC -!- SIMILARITY: Belongs to the nucleoporin Nup84/Nup107 family.
CC {ECO:0000305}.
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DR EMBL; BX284602; CAA91316.1; -; Genomic_DNA.
DR PIR; T20527; T20527.
DR RefSeq; NP_496481.1; NM_064080.5.
DR AlphaFoldDB; Q19131; -.
DR DIP; DIP-25365N; -.
DR STRING; 6239.F07A11.3; -.
DR EPD; Q19131; -.
DR PaxDb; Q19131; -.
DR PeptideAtlas; Q19131; -.
DR EnsemblMetazoa; F07A11.3.1; F07A11.3.1; WBGene00003791.
DR GeneID; 174779; -.
DR KEGG; cel:CELE_F07A11.3; -.
DR UCSC; F07A11.3; c. elegans.
DR CTD; 174779; -.
DR WormBase; F07A11.3; CE03145; WBGene00003791; npp-5.
DR eggNOG; KOG1964; Eukaryota.
DR GeneTree; ENSGT00390000012080; -.
DR HOGENOM; CLU_347244_0_0_1; -.
DR InParanoid; Q19131; -.
DR OMA; WAYANSA; -.
DR OrthoDB; 316194at2759; -.
DR Reactome; R-CEL-159227; Transport of the SLBP independent Mature mRNA.
DR Reactome; R-CEL-159230; Transport of the SLBP Dependant Mature mRNA.
DR Reactome; R-CEL-159231; Transport of Mature mRNA Derived from an Intronless Transcript.
DR Reactome; R-CEL-159236; Transport of Mature mRNA derived from an Intron-Containing Transcript.
DR Reactome; R-CEL-191859; snRNP Assembly.
DR Reactome; R-CEL-3108214; SUMOylation of DNA damage response and repair proteins.
DR Reactome; R-CEL-3301854; Nuclear Pore Complex (NPC) Disassembly.
DR Reactome; R-CEL-3371453; Regulation of HSF1-mediated heat shock response.
DR Reactome; R-CEL-4085377; SUMOylation of SUMOylation proteins.
DR Reactome; R-CEL-4551638; SUMOylation of chromatin organization proteins.
DR Reactome; R-CEL-9615933; Postmitotic nuclear pore complex (NPC) reformation.
DR PRO; PR:Q19131; -.
DR Proteomes; UP000001940; Chromosome II.
DR Bgee; WBGene00003791; Expressed in germ line (C elegans) and 4 other tissues.
DR GO; GO:0000776; C:kinetochore; IEA:UniProtKB-KW.
DR GO; GO:0031965; C:nuclear membrane; IMP:UniProtKB.
DR GO; GO:0005643; C:nuclear pore; IDA:UniProtKB.
DR GO; GO:0031080; C:nuclear pore outer ring; IBA:GO_Central.
DR GO; GO:0017056; F:structural constituent of nuclear pore; IBA:GO_Central.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007059; P:chromosome segregation; IEA:UniProtKB-KW.
DR GO; GO:0051382; P:kinetochore assembly; IMP:UniProtKB.
DR GO; GO:0006406; P:mRNA export from nucleus; IBA:GO_Central.
DR GO; GO:0002119; P:nematode larval development; IMP:UniProtKB.
DR GO; GO:0000973; P:post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery; IBA:GO_Central.
DR GO; GO:0006606; P:protein import into nucleus; IBA:GO_Central.
DR GO; GO:0008104; P:protein localization; IMP:UniProtKB.
DR GO; GO:0034501; P:protein localization to kinetochore; IMP:UniProtKB.
DR GO; GO:1900037; P:regulation of cellular response to hypoxia; IMP:UniProtKB.
DR GO; GO:0010965; P:regulation of mitotic sister chromatid separation; IMP:UniProtKB.
DR GO; GO:0097298; P:regulation of nucleus size; IMP:UniProtKB.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR InterPro; IPR007252; Nup84/Nup107.
DR PANTHER; PTHR13003; PTHR13003; 1.
DR Pfam; PF04121; Nup84_Nup100; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Centromere; Chromosome; Chromosome partition;
KW Kinetochore; Membrane; Mitosis; mRNA transport; Nuclear pore complex;
KW Nucleus; Protein transport; Reference proteome; Translocation; Transport.
FT CHAIN 1..813
FT /note="Nuclear pore complex protein 5"
FT /evidence="ECO:0000305"
FT /id="PRO_0000440239"
SQ SEQUENCE 813 AA; 91812 MW; 3F961FAA1991A42C CRC64;
MTDLFASGDN SSNSFDDSND EIARKNETAF KYTTIFHTNL SEKLYHELCA LAFGEFDIPQ
GQISPLMWTR LHEIYSEVEM QTRKLPAGSN YSASSQKYAN EAVQIASVLS IYTALAHEYD
ETVEVSLLSK LVVEDVEFRR IYALLLWSEK AISEQQYEKG LGDKVRKLEG IKSSRINSMM
ALKRPTFGAA NAPKDASLDP DAPGTKEDQA LEQMAMNVFF QLIRSGETSK AANLAIDLGM
GAIGAQLQLH SMLRNPLDIP LEASKQNFGE YKRSRRAKYY QMTQKLIEQS QGSEDDAYWM
LISAIRGNIQ PMLKAGKSVI EKVWAYANSA VLARILAAEG AMTQETISTL FNVPLTSKSI
LDELRSEADR TKEVYILLRV IDDMLNDDIE DLYKFANETV GEFVPNDKNC QVNMLALDIF
FHLVAVSYAS GFEPNDDGNA VIILGFDDLR ARSGTSSHKK MAAFYSRFLP EDMKLPEIVE
TMKAVDSDEE REILAESLKQ SDIDFGRCAC TLIEQIRKDD KTKVVTLEEQ IDHWHWLLIG
GEETALAALE ECNRLVRKVM LSTPIDESVI RQIIRKALHF EVPKLLSQAV ENEATVLSLI
TDGTLFEQKP GSQLAINKIE HAALEFYGLC SFVDVNNFMI TIALKLGLMF KYTPITDDEL
SMIGGVKRLD NTTAADWEAS LRVRARAEQT LREEVLKKRA AEHNTRVGMV QQHLDTVLPM
LRGLVNNIGV RPEYFLSPRA NGDPMRVHRK EIQEIRNLFL PQFFILLAQA AVRLDDTTNF
NDFFTSFNND LGLDQEWMVF IKAFYAELNL KVE
