NPR1_ORYSI
ID NPR1_ORYSI Reviewed; 582 AA.
AC Q5D0W8; B8ADQ5;
DT 07-SEP-2016, integrated into UniProtKB/Swiss-Prot.
DT 29-MAR-2005, sequence version 1.
DT 25-MAY-2022, entry version 88.
DE RecName: Full=BTB/POZ domain and ankyrin repeat-containing protein NPR1 {ECO:0000305};
DE Short=OsNPR1 {ECO:0000303|Ref.2};
DE AltName: Full=NPR1 homolog 1 {ECO:0000305};
DE Short=OsNH1 {ECO:0000303|PubMed:15986920};
GN Name=NPR1 {ECO:0000303|Ref.2}; Synonyms=NH1 {ECO:0000303|PubMed:15986920};
GN ORFNames=OsI_00749 {ECO:0000312|EMBL:EEC70102.1};
OS Oryza sativa subsp. indica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39946;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH TGA2.2, AND
RP MUTAGENESIS OF CYS-150 AND HIS-338.
RC STRAIN=cv. IRBB21;
RX PubMed=15986920; DOI=10.1094/mpmi-18-0511;
RA Chern M., Fitzgerald H.A., Canlas P.E., Navarre D.A., Ronald P.C.;
RT "Overexpression of a rice NPR1 homolog leads to constitutive activation of
RT defense response and hypersensitivity to light.";
RL Mol. Plant Microbe Interact. 18:511-520(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], AND FUNCTION.
RC STRAIN=cv. Gui99;
RX DOI=10.1007/s10658-011-9801-7;
RA Feng J.X., Cao L., Li J., Duan C.J., Luo X.M., Le N., Wei H.H., Liang S.J.,
RA Chu C.C., Pan Q.H., Tang J.L.;
RT "Involvement of OsNPR1/NH1 in rice basal resistance to blast fungus
RT Magnaporthe oryzae.";
RL Eur. J. Plant Pathol. 131:221-235(2011).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. 93-11;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [4]
RP INTERACTION WITH NRR.
RX PubMed=16115061; DOI=10.1111/j.1365-313x.2005.02485.x;
RA Chern M., Canlas P.E., Fitzgerald H.A., Ronald P.C.;
RT "Rice NRR, a negative regulator of disease resistance, interacts with
RT Arabidopsis NPR1 and rice NH1.";
RL Plant J. 43:623-635(2005).
CC -!- FUNCTION: Key positive factor of disease resistance (PubMed:15986920,
CC Ref.2). Involved in defense response against the bacterial blight
CC disease caused by Xanthomonas oryzae pv. oryzae (Xoo). Plants over-
CC expressing NPR1/NH1 acquire high levels of resistance to Xoo, express
CC constitutively defense genes and develop lesion-mimic spots on leaves
CC at pre-flowering stage (PubMed:15986920). Involved in basal resistance
CC to the blast pathogen Magnaporthe oryzae. Plants over-expressing
CC NPR1/NH1 have increased resistance to M.oryzae infection (Ref.2). Plays
CC an essential role in benzothiadiazole (BTH)-induced resistance to the
CC blast fungus disease caused by Magnaporthe oryzae (By similarity).
CC Functions as a transcriptional coactivator of TGA2.1 and LG2 in vitro
CC (By similarity). Involved in defense response against herbivore. Plants
CC silencing NPR1/NH1 have increased herbivore-induced trypsin proteinase
CC inhibitors and volatiles, which reduces the performance of the striped
CC stem borer (SSB) Chilo suppressalis (By similarity).
CC {ECO:0000250|UniProtKB:Q9FDY4, ECO:0000269|PubMed:15986920,
CC ECO:0000269|Ref.2}.
CC -!- SUBUNIT: Oligomer in an uninduced state; disulfide-linked. Forms
CC activated monomer upon changes in cellular redox potential (By
CC similarity). Interacts with TGA2.2 (Ref.2). Interacts with NRR
CC (PubMed:16115061). {ECO:0000250|UniProtKB:Q9FDY4,
CC ECO:0000269|PubMed:16115061, ECO:0000269|Ref.2}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Nucleus
CC {ECO:0000250|UniProtKB:Q9FDY4}. Note=Accumulation in nucleus when
CC present as monomer. {ECO:0000250|UniProtKB:Q9FDY4}.
CC -!- SEQUENCE CAUTION:
CC Sequence=EEC70102.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AY923983; AAX18700.1; -; mRNA.
DR EMBL; GU722159; ADE05560.1; -; Genomic_DNA.
DR EMBL; GU722160; ADE05561.1; -; mRNA.
DR EMBL; CM000126; EEC70102.1; ALT_SEQ; Genomic_DNA.
DR AlphaFoldDB; Q5D0W8; -.
DR SMR; Q5D0W8; -.
DR STRING; 39946.Q5D0W8; -.
DR Proteomes; UP000007015; Chromosome 1.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:2000022; P:regulation of jasmonic acid mediated signaling pathway; IEA:InterPro.
DR GO; GO:2000031; P:regulation of salicylic acid mediated signaling pathway; IEA:InterPro.
DR GO; GO:0009862; P:systemic acquired resistance, salicylic acid mediated signaling pathway; IEA:InterPro.
DR Gene3D; 1.25.40.20; -; 1.
DR Gene3D; 3.30.710.10; -; 1.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR InterPro; IPR000210; BTB/POZ_dom.
DR InterPro; IPR044292; NPR.
DR InterPro; IPR021094; NPR1/NIM1-like_C.
DR InterPro; IPR024228; NPR_central_dom.
DR InterPro; IPR011333; SKP1/BTB/POZ_sf.
DR PANTHER; PTHR46475; PTHR46475; 1.
DR Pfam; PF13637; Ank_4; 1.
DR Pfam; PF00651; BTB; 1.
DR Pfam; PF11900; DUF3420; 1.
DR Pfam; PF12313; NPR1_like_C; 1.
DR SMART; SM00248; ANK; 3.
DR SMART; SM00225; BTB; 1.
DR SUPFAM; SSF48403; SSF48403; 1.
DR SUPFAM; SSF54695; SSF54695; 1.
DR PROSITE; PS50297; ANK_REP_REGION; 1.
DR PROSITE; PS50088; ANK_REPEAT; 1.
DR PROSITE; PS50097; BTB; 1.
PE 1: Evidence at protein level;
KW ANK repeat; Cytoplasm; Disulfide bond; Nucleus; Plant defense;
KW Reference proteome; Repeat.
FT CHAIN 1..582
FT /note="BTB/POZ domain and ankyrin repeat-containing protein
FT NPR1"
FT /id="PRO_0000436999"
FT DOMAIN 55..140
FT /note="BTB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00037"
FT REPEAT 269..299
FT /note="ANK 1"
FT /evidence="ECO:0000255"
FT REPEAT 301..328
FT /note="ANK 2"
FT /evidence="ECO:0000255"
FT REPEAT 332..361
FT /note="ANK 3"
FT /evidence="ECO:0000255"
FT REGION 1..27
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 525..544
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 551..582
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..17
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 565..582
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT DISULFID 76
FT /note="Interchain (with C-216); in linked form"
FT /evidence="ECO:0000250|UniProtKB:P93002"
FT DISULFID 216
FT /note="Interchain (with C-76); in linked form"
FT /evidence="ECO:0000250|UniProtKB:P93002"
FT MUTAGEN 150
FT /note="C->Y: Abolishes the interaction with TGA2.2."
FT /evidence="ECO:0000269|PubMed:15986920"
FT MUTAGEN 338
FT /note="H->Y: Abolishes the interaction with TGA2.2."
FT /evidence="ECO:0000269|PubMed:15986920"
SQ SEQUENCE 582 AA; 63897 MW; E895DAB994D309F1 CRC64;
MEPPTSHVTN AFSDSDSASV EEGDADADAD VEALRRLSDN LAAAFRSPED FAFLADARIA
VPGGGGGGGD LRVHRCVLSA RSPFLRGVFA RRAAAAAGGG GEDGSERLEL RELLGGGGEE
VEVGYEALRL VLDYLYSGRV GDLPKAACLC VDEDCAHVGC HPAVAFMAQV LFAASTFQVA
ELTNLFQRRL LDVLDKVEVD NLLLILSVAN LCNKSCMKLL ERCLDMVVRS NLDMITLEKS
LPPDVIKQII DARLSLGLIS PENKGFPNKH VRRIHRALDS DDVELVRMLL TEGQTNLDDA
FALHYAVEHC DSKITTELLD LALADVNHRN PRGYTVLHIA ARRREPKIIV SLLTKGARPA
DVTFDGRKAV QISKRLTKQG DYFGVTEEGK PSPKDRLCIE ILEQAERRDP QLGEASVSLA
MAGESLRGRL LYLENRVALA RIMFPMEARV AMDIAQVDGT LEFNLGSGAN PPPERQRTTV
DLNESPFIMK EEHLARMTAL SKTVELGKRF FPRCSNVLDK IMDDETDPVS LGRDTSAEKR
KRFHDLQDVL QKAFHEDKEE NDRSGLSSSS SSTSIGAIRP RR
