ID   NPR2_ARATH              Reviewed;         600 AA.
AC   Q9SZI3;
DT   03-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 137.
DE   RecName: Full=Regulatory protein NPR2;
DE   AltName: Full=BTB/POZ domain-containing protein NPR2;
GN   Name=NPR2; OrderedLocusNames=At4g26120; ORFNames=F20B18.230;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617198; DOI=10.1038/47134;
RA   Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA   Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA   Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA   de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA   Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA   Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA   Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA   Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA   Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA   Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA   Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA   Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA   Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA   Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA   Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA   Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA   Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA   Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA   Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA   Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA   Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA   Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA   Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA   Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA   Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA   Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA   Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA   de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA   Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA   Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA   Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA   Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA   Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA   Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA   Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA   Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA   Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA   O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA   Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA   Martienssen R., McCombie W.R.;
RT   "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL   Nature 402:769-777(1999).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   DOMAIN BTB.
RX   PubMed=15749712; DOI=10.1074/jbc.m413247200;
RA   Gingerich D.J., Gagne J.M., Salter D.W., Hellmann H., Estelle M., Ma L.,
RA   Vierstra R.D.;
RT   "Cullins 3a and 3b assemble with members of the broad
RT   complex/tramtrack/bric-a-brac (BTB) protein family to form essential
RT   ubiquitin-protein ligases (E3s) in Arabidopsis.";
RL   J. Biol. Chem. 280:18810-18821(2005).
RN   [4]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=15634206; DOI=10.1111/j.1365-313x.2004.02296.x;
RA   Liu G., Holub E.B., Alonso J.M., Ecker J.R., Fobert P.R.;
RT   "An Arabidopsis NPR1-like gene, NPR4, is required for disease resistance.";
RL   Plant J. 41:304-318(2005).
CC   -!- FUNCTION: May act as a substrate-specific adapter of an E3 ubiquitin-
CC       protein ligase complex (CUL3-RBX1-BTB) which mediates the
CC       ubiquitination and subsequent proteasomal degradation of target
CC       proteins. {ECO:0000250}.
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC   -!- INTERACTION:
CC       Q9SZI3; P93002: NPR1; NbExp=2; IntAct=EBI-15987489, EBI-1392127;
CC   -!- DOMAIN: The BTB/POZ domain mediates the interaction with some component
CC       of ubiquitin ligase complexes. {ECO:0000269|PubMed:15749712}.
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DR   EMBL; AL049483; CAB39677.1; -; Genomic_DNA.
DR   EMBL; AL161564; CAB79467.1; -; Genomic_DNA.
DR   EMBL; CP002687; AEE85160.1; -; Genomic_DNA.
DR   PIR; T04267; T04267.
DR   RefSeq; NP_194342.1; NM_118745.2.
DR   AlphaFoldDB; Q9SZI3; -.
DR   SMR; Q9SZI3; -.
DR   DIP; DIP-59899N; -.
DR   IntAct; Q9SZI3; 1.
DR   STRING; 3702.AT4G26120.1; -.
DR   PaxDb; Q9SZI3; -.
DR   PRIDE; Q9SZI3; -.
DR   EnsemblPlants; AT4G26120.1; AT4G26120.1; AT4G26120.
DR   GeneID; 828718; -.
DR   Gramene; AT4G26120.1; AT4G26120.1; AT4G26120.
DR   KEGG; ath:AT4G26120; -.
DR   Araport; AT4G26120; -.
DR   TAIR; locus:2120800; AT4G26120.
DR   eggNOG; KOG0504; Eukaryota.
DR   HOGENOM; CLU_034895_1_0_1; -.
DR   InParanoid; Q9SZI3; -.
DR   OMA; ANICGET; -.
DR   PhylomeDB; Q9SZI3; -.
DR   UniPathway; UPA00143; -.
DR   PRO; PR:Q9SZI3; -.
DR   Proteomes; UP000006548; Chromosome 4.
DR   ExpressionAtlas; Q9SZI3; baseline and differential.
DR   Genevisible; Q9SZI3; AT.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0042742; P:defense response to bacterium; IBA:GO_Central.
DR   GO; GO:0050832; P:defense response to fungus; IBA:GO_Central.
DR   GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR   GO; GO:2000022; P:regulation of jasmonic acid mediated signaling pathway; IBA:GO_Central.
DR   GO; GO:2000031; P:regulation of salicylic acid mediated signaling pathway; IEA:InterPro.
DR   GO; GO:0009862; P:systemic acquired resistance, salicylic acid mediated signaling pathway; IEA:InterPro.
DR   Gene3D; 1.25.40.20; -; 1.
DR   Gene3D; 3.30.710.10; -; 1.
DR   InterPro; IPR002110; Ankyrin_rpt.
DR   InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR   InterPro; IPR000210; BTB/POZ_dom.
DR   InterPro; IPR044292; NPR.
DR   InterPro; IPR021094; NPR1/NIM1-like_C.
DR   InterPro; IPR024228; NPR_central_dom.
DR   InterPro; IPR011333; SKP1/BTB/POZ_sf.
DR   PANTHER; PTHR46475; PTHR46475; 1.
DR   Pfam; PF00023; Ank; 1.
DR   Pfam; PF00651; BTB; 1.
DR   Pfam; PF11900; DUF3420; 1.
DR   Pfam; PF12313; NPR1_like_C; 1.
DR   SMART; SM00248; ANK; 2.
DR   SMART; SM00225; BTB; 1.
DR   SUPFAM; SSF48403; SSF48403; 1.
DR   SUPFAM; SSF54695; SSF54695; 1.
DR   PROSITE; PS50297; ANK_REP_REGION; 1.
DR   PROSITE; PS50088; ANK_REPEAT; 1.
DR   PROSITE; PS50097; BTB; 1.
PE   1: Evidence at protein level;
KW   ANK repeat; Phosphoprotein; Reference proteome; Repeat; S-nitrosylation;
KW   Ubl conjugation pathway.
FT   CHAIN           1..600
FT                   /note="Regulatory protein NPR2"
FT                   /id="PRO_0000407991"
FT   DOMAIN          67..143
FT                   /note="BTB"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00037"
FT   REPEAT          264..294
FT                   /note="ANK 1"
FT   REPEAT          295..323
FT                   /note="ANK 2"
FT   REPEAT          327..356
FT                   /note="ANK 3"
FT   REGION          554..600
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        585..600
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         15
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P93002"
FT   MOD_RES         19
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P93002"
FT   MOD_RES         155
FT                   /note="S-nitrosocysteine"
FT                   /evidence="ECO:0000250|UniProtKB:P93002"
SQ   SEQUENCE   600 AA;  68006 MW;  E32D3A1ED62ACB74 CRC64;
     MATTTTTTTA RFSDSYEFSN TSGNSFFAAE SSLDYPTEFL TPPEVSALKL LSNCLESVFD
     SPETFYSDAK LVLAGGREVS FHRCILSARI PVFKSALATV KEQKSSTTVK LQLKEIARDY
     EVGFDSVVAV LAYVYSGRVR SPPKGASACV DDDCCHVACR SKVDFMVEVL YLSFVFQIQE
     LVTLYERQFL EIVDKVVVED ILVIFKLDTL CGTTYKKLLD RCIEIIVKSD IELVSLEKSL
     PQHIFKQIID IREALCLEPP KLERHVKNIY KALDSDDVEL VKMLLLEGHT NLDEAYALHF
     AIAHCAVKTA YDLLELELAD VNLRNPRGYT VLHVAAMRKE PKLIISLLMK GANILDTTLD
     GRTALVIVKR LTKADDYKTS TEDGTPSLKG GLCIEVLEHE QKLEYLSPIE ASLSLPVTPE
     ELRMRLLYYE NRVALARLLF PVETETVQGI AKLEETCEFT ASSLEPDHHI GEKRTSLDLN
     MAPFQIHEKH LSRLRALCKT VELGKRYFKR CSLDHFMDTE DLNHLASVEE DTPEKRLQKK
     QRYMELQETL MKTFSEDKEE CGKSSTPKPT SAVRSNRKLS HRRLKVDKRD FLKRPYGNGD