NPR6_ARATH
ID NPR6_ARATH Reviewed; 467 AA.
AC Q9M1I7;
DT 03-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Regulatory protein NPR6;
DE AltName: Full=BTB/POZ domain-containing protein NPR6;
DE AltName: Full=Protein BLADE-ON-PETIOLE 1;
GN Name=NPR6; Synonyms=BOP1; OrderedLocusNames=At3g57130; ORFNames=F24I3.210;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W.,
RA Redman J.C., Wu H.C., Utterback T., Town C.D.;
RL Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF Clones.";
RL Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Fujita M., Mizukado S., Seki M., Shinozaki K., Mitsuda N., Takiguchi Y.,
RA Takagi M.;
RT "ORF cloning and analysis of Arabidopsis transcription factor genes.";
RL Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION.
RX PubMed=12441300; DOI=10.1242/dev.00196;
RA Ha C.M., Kim G.T., Kim B.C., Jun J.H., Soh M.S., Ueno Y., Machida Y.,
RA Tsukaya H., Nam H.G.;
RT "The BLADE-ON-PETIOLE 1 gene controls leaf pattern formation through the
RT modulation of meristematic activity in Arabidopsis.";
RL Development 130:161-172(2003).
RN [7]
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=15564519; DOI=10.1093/pcp/pch201;
RA Ha C.M., Jun J.H., Nam H.G., Fletcher J.C.;
RT "BLADE-ON-PETIOLE1 encodes a BTB/POZ domain protein required for leaf
RT morphogenesis in Arabidopsis thaliana.";
RL Plant Cell Physiol. 45:1361-1370(2004).
RN [8]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=15800002; DOI=10.1242/dev.01815;
RA Norberg M., Holmlund M., Nilsson O.;
RT "The BLADE ON PETIOLE genes act redundantly to control the growth and
RT development of lateral organs.";
RL Development 132:2203-2213(2005).
RN [9]
RP DOMAIN BTB.
RX PubMed=15749712; DOI=10.1074/jbc.m413247200;
RA Gingerich D.J., Gagne J.M., Salter D.W., Hellmann H., Estelle M., Ma L.,
RA Vierstra R.D.;
RT "Cullins 3a and 3b assemble with members of the broad
RT complex/tramtrack/bric-a-brac (BTB) protein family to form essential
RT ubiquitin-protein ligases (E3s) in Arabidopsis.";
RL J. Biol. Chem. 280:18810-18821(2005).
RN [10]
RP FUNCTION, DISRUPTION PHENOTYPE, AND INTERACTION WITH PAN.
RX PubMed=15805484; DOI=10.1105/tpc.104.030536;
RA Hepworth S.R., Zhang Y., McKim S., Li X., Haughn G.W.;
RT "BLADE-ON-PETIOLE-dependent signaling controls leaf and floral patterning
RT in Arabidopsis.";
RL Plant Cell 17:1434-1448(2005).
RN [11]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=15634206; DOI=10.1111/j.1365-313x.2004.02296.x;
RA Liu G., Holub E.B., Alonso J.M., Ecker J.R., Fobert P.R.;
RT "An Arabidopsis NPR1-like gene, NPR4, is required for disease resistance.";
RL Plant J. 41:304-318(2005).
RN [12]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=17601823; DOI=10.1105/tpc.107.051938;
RA Ha C.M., Jun J.H., Nam H.G., Fletcher J.C.;
RT "BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through
RT regulation of LOB domain and adaxial-abaxial polarity genes.";
RL Plant Cell 19:1809-1825(2007).
RN [13]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=18339677; DOI=10.1242/dev.012807;
RA McKim S.M., Stenvik G.E., Butenko M.A., Kristiansen W., Cho S.K.,
RA Hepworth S.R., Aalen R.B., Haughn G.W.;
RT "The BLADE-ON-PETIOLE genes are essential for abscission zone formation in
RT Arabidopsis.";
RL Development 135:1537-1546(2008).
RN [14]
RP FUNCTION.
RX PubMed=20610407; DOI=10.1534/genetics.110.118703;
RA Ha C.M., Jun J.H., Fletcher J.C.;
RT "Control of Arabidopsis leaf morphogenesis through regulation of the YABBY
RT and KNOX families of transcription factors.";
RL Genetics 186:197-206(2010).
RN [15]
RP FUNCTION, SUBCELLULAR LOCATION, AND SUBUNIT.
RX PubMed=20118228; DOI=10.1105/tpc.109.070763;
RA Jun J.H., Ha C.M., Fletcher J.C.;
RT "BLADE-ON-PETIOLE1 coordinates organ determinacy and axial polarity in
RT arabidopsis by directly activating ASYMMETRIC LEAVES2.";
RL Plant Cell 22:62-76(2010).
RN [16]
RP FUNCTION.
RX PubMed=20626659; DOI=10.1111/j.1365-313x.2010.04299.x;
RA Xu M., Hu T., McKim S.M., Murmu J., Haughn G.W., Hepworth S.R.;
RT "Arabidopsis BLADE-ON-PETIOLE1 and 2 promote floral meristem fate and
RT determinacy in a previously undefined pathway targeting APETALA1 and
RT AGAMOUS-LIKE24.";
RL Plant J. 63:974-989(2010).
CC -!- FUNCTION: May act as a substrate-specific adapter of an E3 ubiquitin-
CC protein ligase complex (CUL3-RBX1-BTB) which mediates the
CC ubiquitination and subsequent proteasomal degradation of target
CC proteins (By similarity). Acts redundantly with BOP2. BOP1/2 promote
CC leaf and floral meristem fate and determinacy in a pathway targeting
CC AP1 and AGL24. BOP1/2 act as transcriptional co-regulators through
CC direct interaction with TGA factors, including PAN, a direct regulator
CC of AP1. Controls lateral organ fate through positive regulation of
CC adaxial-abaxial polarity genes ATHB-14/PHB, YAB1/FIL and YAB3, and
CC through positive regulation of LOB domain-containing genes LOB,
CC LBD6/AS2 and LBD36. Promotes and maintains a developmentally
CC determinate state in leaf cells through the negative regulation of JAG,
CC JGL and class I KNOX genes. Is also involved in nectary development,
CC formation of normal abscission zones and suppression of bract
CC formation. {ECO:0000250, ECO:0000269|PubMed:12441300,
CC ECO:0000269|PubMed:15564519, ECO:0000269|PubMed:15800002,
CC ECO:0000269|PubMed:15805484, ECO:0000269|PubMed:17601823,
CC ECO:0000269|PubMed:18339677, ECO:0000269|PubMed:20118228,
CC ECO:0000269|PubMed:20610407, ECO:0000269|PubMed:20626659}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBUNIT: Homodimer or heterodimer with BOP2. Interacts with PAN.
CC {ECO:0000269|PubMed:15805484, ECO:0000269|PubMed:20118228}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:20118228}. Nucleus
CC {ECO:0000269|PubMed:20118228}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q9M1I7-1; Sequence=Displayed;
CC -!- DEVELOPMENTAL STAGE: Initially detectable in embryos with a
CC localization to the base of the developing cotyledons near the SAM.
CC Expressed during vegetative development in young leaf primordia and at
CC the base of the rosette leaves on the adaxial side. Expressed during
CC reproductive development in young floral buds, and at the base of the
CC sepals and petals. {ECO:0000269|PubMed:15564519}.
CC -!- DOMAIN: The BTB/POZ domain mediates the interaction with some component
CC of ubiquitin ligase complexes. {ECO:0000269|PubMed:15749712}.
CC -!- DISRUPTION PHENOTYPE: Defects in rosette leaf development. bop1 and
CC bop2 double mutant displays leafy petioles, loss of floral organ
CC abscission, and asymmetric flowers subtended by a bract.
CC {ECO:0000269|PubMed:15800002, ECO:0000269|PubMed:15805484,
CC ECO:0000269|PubMed:17601823, ECO:0000269|PubMed:18339677}.
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DR EMBL; AL138655; CAB72183.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE79617.1; -; Genomic_DNA.
DR EMBL; DQ056629; AAY78777.1; -; mRNA.
DR EMBL; BT026363; ABH04470.1; -; mRNA.
DR EMBL; AB493655; BAH30493.1; -; mRNA.
DR PIR; T47773; T47773.
DR RefSeq; NP_191272.1; NM_115572.2. [Q9M1I7-1]
DR AlphaFoldDB; Q9M1I7; -.
DR SMR; Q9M1I7; -.
DR BioGRID; 10196; 3.
DR STRING; 3702.AT3G57130.1; -.
DR PaxDb; Q9M1I7; -.
DR PRIDE; Q9M1I7; -.
DR ProteomicsDB; 250545; -. [Q9M1I7-1]
DR EnsemblPlants; AT3G57130.1; AT3G57130.1; AT3G57130. [Q9M1I7-1]
DR GeneID; 824880; -.
DR Gramene; AT3G57130.1; AT3G57130.1; AT3G57130. [Q9M1I7-1]
DR KEGG; ath:AT3G57130; -.
DR Araport; AT3G57130; -.
DR TAIR; locus:2080595; AT3G57130.
DR eggNOG; KOG0504; Eukaryota.
DR HOGENOM; CLU_028148_0_0_1; -.
DR InParanoid; Q9M1I7; -.
DR OMA; CSYLIAK; -.
DR PhylomeDB; Q9M1I7; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q9M1I7; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9M1I7; baseline and differential.
DR Genevisible; Q9M1I7; AT.
DR GO; GO:0005737; C:cytoplasm; IDA:TAIR.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0010434; P:bract formation; IGI:UniProtKB.
DR GO; GO:0010582; P:floral meristem determinacy; IGI:TAIR.
DR GO; GO:0010227; P:floral organ abscission; IGI:UniProtKB.
DR GO; GO:0048439; P:flower morphogenesis; IGI:TAIR.
DR GO; GO:0009864; P:induced systemic resistance, jasmonic acid mediated signaling pathway; IGI:TAIR.
DR GO; GO:0009965; P:leaf morphogenesis; IMP:UniProtKB.
DR GO; GO:0010022; P:meristem determinacy; IMP:TAIR.
DR GO; GO:0010254; P:nectary development; IGI:TAIR.
DR GO; GO:0009944; P:polarity specification of adaxial/abaxial axis; IGI:TAIR.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR GO; GO:0009954; P:proximal/distal pattern formation; IGI:TAIR.
DR Gene3D; 1.25.40.20; -; 1.
DR Gene3D; 3.30.710.10; -; 1.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR InterPro; IPR000210; BTB/POZ_dom.
DR InterPro; IPR044284; NPR5/6.
DR InterPro; IPR024228; NPR_central_dom.
DR InterPro; IPR011333; SKP1/BTB/POZ_sf.
DR PANTHER; PTHR46668; PTHR46668; 1.
DR Pfam; PF12796; Ank_2; 1.
DR Pfam; PF00651; BTB; 1.
DR Pfam; PF11900; DUF3420; 1.
DR SMART; SM00248; ANK; 2.
DR SMART; SM00225; BTB; 1.
DR SUPFAM; SSF48403; SSF48403; 1.
DR SUPFAM; SSF54695; SSF54695; 1.
DR PROSITE; PS50297; ANK_REP_REGION; 1.
DR PROSITE; PS50088; ANK_REPEAT; 1.
DR PROSITE; PS50097; BTB; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; ANK repeat; Cytoplasm; Nucleus; Reference proteome;
KW Repeat; Ubl conjugation pathway.
FT CHAIN 1..467
FT /note="Regulatory protein NPR6"
FT /id="PRO_0000407995"
FT DOMAIN 27..111
FT /note="BTB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00037"
FT REPEAT 247..276
FT /note="ANK 1"
FT REPEAT 277..306
FT /note="ANK 2"
FT REPEAT 311..340
FT /note="ANK 3"
FT REGION 434..467
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 438..454
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 467 AA; 51785 MW; 1A063A205DF86A62 CRC64;
MSNTFEESLK SMSLDYLNLL INGQAFSDVT FSVEGRLVHA HRCILAARSL FFRKFFCESD
PSQPGAEPAN QTGSGARAAA VGGVIPVNSV GYEVFLLLLQ FLYSGQVSIV PHKHEPRSNC
GDRGCWHTHC TAAVDLSLDI LAAARYFGVE QLALLTQKHL TSMVEKASIE DVMKVLIASR
KQDMHQLWTT CSYLIAKSGL PQEILAKHLP IELVAKIEEL RLKSSMPLRS LMPHHHDLTS
TLDLEDQKIR RMRRALDSSD VELVKLMVMG EGLNLDESLA LIYAVENCSR EVVKALLELG
AADVNYPAGP TGKTALHIAA EMVSPDMVAV LLDHHADPNV QTVDGITPLD ILRTLTSDFL
FKGAIPGLTH IEPNKLRLCL ELVQSAALVI SREEGNNNSN DNNTMIYPRM KDEHTSGSSL
DSRLVYLNLG ATNRDIGDDN SNQREGMNLH HHHHDPSTMY HHHHHHF
