NPS12_ARATH
ID NPS12_ARATH Reviewed; 265 AA.
AC Q9LNH6; Q9SX59;
DT 17-JAN-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Novel plant SNARE 12;
DE Short=AtNPSN12;
GN Name=NPSN12; OrderedLocusNames=At1g48240; ORFNames=F11A17.20, F21D18.4;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=12068098; DOI=10.1104/pp.003970;
RA Zheng H., Bednarek S.Y., Sanderfoot A.A., Alonso J., Ecker J.R.,
RA Raikhel N.V.;
RT "NPSN11 is a cell plate-associated SNARE protein that interacts with the
RT syntaxin KNOLLE.";
RL Plant Physiol. 129:530-539(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
CC -!- FUNCTION: Vesicle trafficking protein that functions in the secretory
CC pathway. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Single-pass type IV
CC membrane protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, stems, flower, siliques and
CC leaves.
CC -!- SIMILARITY: Belongs to the novel plant SNARE family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD49774.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AF487545; AAL92119.1; -; mRNA.
DR EMBL; AC007932; AAD49774.2; ALT_SEQ; Genomic_DNA.
DR EMBL; AC023673; AAF79516.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE32268.1; -; Genomic_DNA.
DR EMBL; BT005271; AAO63335.1; -; mRNA.
DR PIR; C96522; C96522.
DR RefSeq; NP_175258.2; NM_103721.5.
DR AlphaFoldDB; Q9LNH6; -.
DR SMR; Q9LNH6; -.
DR BioGRID; 26469; 3.
DR IntAct; Q9LNH6; 3.
DR STRING; 3702.AT1G48240.1; -.
DR PaxDb; Q9LNH6; -.
DR PRIDE; Q9LNH6; -.
DR ProteomicsDB; 250517; -.
DR EnsemblPlants; AT1G48240.1; AT1G48240.1; AT1G48240.
DR GeneID; 841244; -.
DR Gramene; AT1G48240.1; AT1G48240.1; AT1G48240.
DR KEGG; ath:AT1G48240; -.
DR Araport; AT1G48240; -.
DR TAIR; locus:2007730; AT1G48240.
DR eggNOG; ENOG502QQ25; Eukaryota.
DR HOGENOM; CLU_058321_0_0_1; -.
DR InParanoid; Q9LNH6; -.
DR OMA; FWVTIEK; -.
DR OrthoDB; 1198270at2759; -.
DR PhylomeDB; Q9LNH6; -.
DR PRO; PR:Q9LNH6; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9LNH6; baseline and differential.
DR Genevisible; Q9LNH6; AT.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IBA:GO_Central.
DR GO; GO:0012507; C:ER to Golgi transport vesicle membrane; IBA:GO_Central.
DR GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031902; C:late endosome membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; HDA:TAIR.
DR GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR GO; GO:0031201; C:SNARE complex; IBA:GO_Central.
DR GO; GO:0005484; F:SNAP receptor activity; IBA:GO_Central.
DR GO; GO:0000149; F:SNARE binding; IBA:GO_Central.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0006906; P:vesicle fusion; IBA:GO_Central.
DR CDD; cd15861; SNARE_SNAP25N_23N_29N_SEC9N; 1.
DR InterPro; IPR044766; NPSN/SNAP25-like_N_SNARE.
DR InterPro; IPR000727; T_SNARE_dom.
DR SMART; SM00397; t_SNARE; 1.
DR PROSITE; PS50192; T_SNARE; 1.
PE 2: Evidence at transcript level;
KW Coiled coil; Membrane; Phosphoprotein; Protein transport;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..265
FT /note="Novel plant SNARE 12"
FT /id="PRO_0000213617"
FT TOPO_DOM 1..217
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 218..238
FT /note="Helical; Anchor for type IV membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 239..265
FT /note="Vesicular"
FT /evidence="ECO:0000255"
FT DOMAIN 146..208
FT /note="t-SNARE coiled-coil homology"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00202"
FT COILED 32..106
FT /evidence="ECO:0000255"
FT MOD_RES 74
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9LRP1"
SQ SEQUENCE 265 AA; 29887 MW; AC2B8A7F7C6A3B18 CRC64;
MASELPMSPH LEQIHGEIRD HFRALANGFQ RLDKIKDSSR QSKQLEELAE KMRDCKRLVK
EFDRELKDGE ARNSPQVNKQ LNDEKQSMIK ELNSYVALRK TYLNTLGNKK VELFDTGAGV
SGEPTAEENV QMASTMSNQE LVDAGMKRMD ETDQAIERSK QVVHQTLEVG TQTASNLKGQ
TDQMGRVVND LDTIQFSLKK ASQLVKEIGR QVATDKCIMA FLFLIVCGVI AIIIVKIVNP
NNKDIRDIPG LAPPAQSRKL LYFRE
