NPS13_ARATH
ID NPS13_ARATH Reviewed; 269 AA.
AC Q9LRP1;
DT 17-JAN-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Novel plant SNARE 13;
DE Short=AtNPSN13;
GN Name=NPSN13; OrderedLocusNames=At3g17440; ORFNames=MTO12.3;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-74, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
CC -!- FUNCTION: Vesicle trafficking protein that functions in the secretory
CC pathway. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Single-pass type IV
CC membrane protein {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q9LRP1-1; Sequence=Displayed;
CC -!- SIMILARITY: Belongs to the novel plant SNARE family. {ECO:0000305}.
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DR EMBL; AB028620; BAB02920.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE75953.1; -; Genomic_DNA.
DR EMBL; AY099622; AAM20473.1; -; mRNA.
DR EMBL; BT001249; AAN65136.1; -; mRNA.
DR EMBL; AY084645; AAM61208.1; -; mRNA.
DR RefSeq; NP_566578.1; NM_112623.5. [Q9LRP1-1]
DR AlphaFoldDB; Q9LRP1; -.
DR SMR; Q9LRP1; -.
DR BioGRID; 6341; 34.
DR IntAct; Q9LRP1; 32.
DR STRING; 3702.AT3G17440.1; -.
DR iPTMnet; Q9LRP1; -.
DR SwissPalm; Q9LRP1; -.
DR PaxDb; Q9LRP1; -.
DR PRIDE; Q9LRP1; -.
DR ProteomicsDB; 251121; -. [Q9LRP1-1]
DR EnsemblPlants; AT3G17440.1; AT3G17440.1; AT3G17440. [Q9LRP1-1]
DR GeneID; 821008; -.
DR Gramene; AT3G17440.1; AT3G17440.1; AT3G17440. [Q9LRP1-1]
DR KEGG; ath:AT3G17440; -.
DR Araport; AT3G17440; -.
DR TAIR; locus:2093601; AT3G17440.
DR eggNOG; ENOG502QQ25; Eukaryota.
DR HOGENOM; CLU_058321_0_0_1; -.
DR InParanoid; Q9LRP1; -.
DR OMA; QMTPQLE; -.
DR PhylomeDB; Q9LRP1; -.
DR PRO; PR:Q9LRP1; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LRP1; baseline and differential.
DR Genevisible; Q9LRP1; AT.
DR GO; GO:0005829; C:cytosol; HDA:TAIR.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IBA:GO_Central.
DR GO; GO:0012507; C:ER to Golgi transport vesicle membrane; IBA:GO_Central.
DR GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031902; C:late endosome membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; HDA:TAIR.
DR GO; GO:0031201; C:SNARE complex; IBA:GO_Central.
DR GO; GO:0005484; F:SNAP receptor activity; IBA:GO_Central.
DR GO; GO:0000149; F:SNARE binding; IBA:GO_Central.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0006906; P:vesicle fusion; IBA:GO_Central.
DR CDD; cd15861; SNARE_SNAP25N_23N_29N_SEC9N; 1.
DR InterPro; IPR044766; NPSN/SNAP25-like_N_SNARE.
DR InterPro; IPR000727; T_SNARE_dom.
DR SMART; SM00397; t_SNARE; 1.
DR PROSITE; PS50192; T_SNARE; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Membrane; Phosphoprotein;
KW Protein transport; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..269
FT /note="Novel plant SNARE 13"
FT /id="PRO_0000213618"
FT TOPO_DOM 1..217
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 218..238
FT /note="Helical; Anchor for type IV membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 239..269
FT /note="Vesicular"
FT /evidence="ECO:0000255"
FT DOMAIN 146..208
FT /note="t-SNARE coiled-coil homology"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00202"
FT COILED 33..94
FT /evidence="ECO:0000255"
FT MOD_RES 74
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19376835"
FT CONFLICT 188..189
FT /note="VN -> D (in Ref. 4; AAM20473)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 269 AA; 30414 MW; 2238C876A48ACAAC CRC64;
MASNLPMSPQ LEQIHGEIRD HFRALANGFQ RLDKIKDSTR QSKQLEELTD KMRECKRLVK
EFDRELKDEE ARNSPEVNKQ LNDEKQSMIK ELNSYVALRK TYMSTLGNKK VELFDMGAGV
SGEPTAEENV QVASSMSNQE LVDAGMKRMD ETDQAIERSK QVVEQTLEVG TQTAANLKGQ
TDQMGRVVNH LDTIQFSIKK ASQLVKEIGR QVATDKCIMG FLFLIVCGVV AIIIVKIVNP
NNKDIRDIPG LAPPAQSRKL LYLRNQDYM
