NPS2_COCH4
ID NPS2_COCH4 Reviewed; 5386 AA.
AC Q5D6D7; N4WT87;
DT 20-JUN-2018, integrated into UniProtKB/Swiss-Prot.
DT 20-JUN-2018, sequence version 2.
DT 23-FEB-2022, entry version 81.
DE RecName: Full=Nonribosomal peptide synthetase 2 {ECO:0000303|PubMed:15755917};
DE Short=NPRS 2 {ECO:0000303|PubMed:15755917};
DE EC=6.3.2.- {ECO:0000305|PubMed:15755917};
DE AltName: Full=Ferricrocin synthetase {ECO:0000303|PubMed:17601875};
DE AltName: Full=Intracellular siderophore synthetase {ECO:0000303|PubMed:17601875};
GN Name=NPS2 {ECO:0000303|PubMed:15755917};
OS Cochliobolus heterostrophus (strain C4 / ATCC 48331 / race T) (Southern
OS corn leaf blight fungus) (Bipolaris maydis).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes;
OC Pleosporomycetidae; Pleosporales; Pleosporineae; Pleosporaceae; Bipolaris.
OX NCBI_TaxID=665024;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND DOMAIN.
RC STRAIN=C4 / ATCC 48331 / race T;
RX PubMed=15755917; DOI=10.1128/ec.4.3.545-555.2005;
RA Lee B.N., Kroken S., Chou D.Y., Robbertse B., Yoder O.C., Turgeon B.G.;
RT "Functional analysis of all nonribosomal peptide synthetases in
RT Cochliobolus heterostrophus reveals a factor, NPS6, involved in virulence
RT and resistance to oxidative stress.";
RL Eukaryot. Cell 4:545-555(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C4 / ATCC 48331 / race T;
RX PubMed=23236275; DOI=10.1371/journal.ppat.1003037;
RA Ohm R.A., Feau N., Henrissat B., Schoch C.L., Horwitz B.A., Barry K.W.,
RA Condon B.J., Copeland A.C., Dhillon B., Glaser F., Hesse C.N., Kosti I.,
RA LaButti K., Lindquist E.A., Lucas S., Salamov A.A., Bradshaw R.E.,
RA Ciuffetti L., Hamelin R.C., Kema G.H.J., Lawrence C., Scott J.A.,
RA Spatafora J.W., Turgeon B.G., de Wit P.J.G.M., Zhong S., Goodwin S.B.,
RA Grigoriev I.V.;
RT "Diverse lifestyles and strategies of plant pathogenesis encoded in the
RT genomes of eighteen Dothideomycetes fungi.";
RL PLoS Pathog. 8:E1003037-E1003037(2012).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C4 / ATCC 48331 / race T;
RX PubMed=23357949; DOI=10.1371/journal.pgen.1003233;
RA Condon B.J., Leng Y., Wu D., Bushley K.E., Ohm R.A., Otillar R., Martin J.,
RA Schackwitz W., Grimwood J., MohdZainudin N., Xue C., Wang R., Manning V.A.,
RA Dhillon B., Tu Z.J., Steffenson B.J., Salamov A., Sun H., Lowry S.,
RA LaButti K., Han J., Copeland A., Lindquist E., Barry K., Schmutz J.,
RA Baker S.E., Ciuffetti L.M., Grigoriev I.V., Zhong S., Turgeon B.G.;
RT "Comparative genome structure, secondary metabolite, and effector coding
RT capacity across Cochliobolus pathogens.";
RL PLoS Genet. 9:E1003233-E1003233(2013).
RN [4]
RP FUNCTION.
RX PubMed=17056706; DOI=10.1105/tpc.106.045633;
RA Oide S., Moeder W., Krasnoff S., Gibson D., Haas H., Yoshioka K.,
RA Turgeon B.G.;
RT "NPS6, encoding a nonribosomal peptide synthetase involved in siderophore-
RT mediated iron metabolism, is a conserved virulence determinant of plant
RT pathogenic ascomycetes.";
RL Plant Cell 18:2836-2853(2006).
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, AND PATHWAY.
RX PubMed=17601875; DOI=10.1128/ec.00111-07;
RA Oide S., Krasnoff S.B., Gibson D.M., Turgeon B.G.;
RT "Intracellular siderophores are essential for ascomycete sexual development
RT in heterothallic Cochliobolus heterostrophus and homothallic Gibberella
RT zeae.";
RL Eukaryot. Cell 6:1339-1353(2007).
RN [6]
RP INDUCTION.
RX PubMed=23980626; DOI=10.1094/mpmi-02-13-0055-r;
RA Zhang N., MohdZainudin N.A., Scher K., Condon B.J., Horwitz B.A.,
RA Turgeon B.G.;
RT "Iron, oxidative stress, and virulence: roles of iron-sensitive
RT transcription factor Sre1 and the redox sensor ChAp1 in the maize pathogen
RT Cochliobolus heterostrophus.";
RL Mol. Plant Microbe Interact. 26:1473-1485(2013).
CC -!- FUNCTION: Nonribosomal peptide synthetase; part of the gene cluster
CC that mediates the biosynthesis of hydroxamate-containing siderophores
CC that play a critical role in virulence (PubMed:15755917,
CC PubMed:17056706, PubMed:23980626). Cochliobolus heterostrophus produces
CC extracellular coprogen-type siderophores including coprogen,
CC neocoprogen I and neocoprogen II, as well as the intracellular
CC siderophore ferricrocin (PubMed:17056706). The role of extracellular
CC siderophores is to supply iron to the fungus during plant infection,
CC and the intracellular ferricrocin is required for intracellular iron
CC distribution and storage with a crucial role in ascus and ascospore
CC development (PubMed:17056706, PubMed:17601875). SIDA2 catalyzes the
CC conversion of L-ornithine to N(5)-hydroxyornithine, the first step in
CC the biosynthesis of all hydroxamate-containing siderophores
CC (PubMed:23980626). The assembly of extracellular coprogen-type
CC siderophores is then performed by the nonribosomal peptide synthetase
CC (NRPS) NPS6 whereas the intracellular siderophore ferricrocin is
CC assembled by NPS2 (PubMed:17056706, PubMed:17601875).
CC {ECO:0000269|PubMed:15755917, ECO:0000269|PubMed:17056706,
CC ECO:0000269|PubMed:17601875}.
CC -!- PATHWAY: Siderophore biosynthesis. {ECO:0000269|PubMed:17601875}.
CC -!- INDUCTION: Expression is repressed by the transcription repressor SRE1
CC under iron replete conditions (PubMed:23980626).
CC {ECO:0000269|PubMed:23980626}.
CC -!- DOMAIN: NRP synthetases are composed of discrete domains (adenylation
CC (A), thiolation (T) or peptidyl carrier protein (PCP) and condensation
CC (C) domains) which when grouped together are referred to as a single
CC module (By similarity). Each module is responsible for the recognition
CC (via the A domain) and incorporation of a single amino acid into the
CC growing peptide product (By similarity). Thus, an NRP synthetase is
CC generally composed of one or more modules and can terminate in a
CC thioesterase domain (TE) that releases the newly synthesized peptide
CC from the enzyme (By similarity). Occasionally, methyltransferase
CC domains (responsible for amino acid methylation) are present within the
CC NRP synthetase (By similarity). NPS2 has the following architecture: A-
CC T-C-A-T-C-A-T-C-A-T-C-T-C-T-C (PubMed:15755917).
CC {ECO:0000250|UniProtKB:A0A144KPJ6, ECO:0000305|PubMed:15755917}.
CC -!- DISRUPTION PHENOTYPE: Leads to defective intracellular siderophore
CC (ferricrocin) biosynthesis and sexual development (PubMed:17601875).
CC {ECO:0000269|PubMed:17601875}.
CC -!- SIMILARITY: Belongs to the NRP synthetase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAX09984.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AY884187; AAX09984.1; ALT_SEQ; Genomic_DNA.
DR EMBL; KB733486; ENH99442.1; -; Genomic_DNA.
DR RefSeq; XP_014073329.1; XM_014217854.1.
DR SMR; Q5D6D7; -.
DR EnsemblFungi; ENH99442; ENH99442; COCC4DRAFT_45281.
DR GeneID; 25845193; -.
DR HOGENOM; CLU_000092_2_0_1; -.
DR OrthoDB; 4243at2759; -.
DR PHI-base; PHI:4234; -.
DR Proteomes; UP000012338; Unassembled WGS sequence.
DR GO; GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0008152; P:metabolic process; IEA:UniProtKB-KW.
DR Gene3D; 1.10.1200.10; -; 6.
DR Gene3D; 3.30.300.30; -; 4.
DR Gene3D; 3.30.559.10; -; 6.
DR Gene3D; 3.40.50.12780; -; 4.
DR InterPro; IPR010071; AA_adenyl_domain.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig.
DR InterPro; IPR042099; ANL_N_sf.
DR InterPro; IPR023213; CAT-like_dom_sf.
DR InterPro; IPR001242; Condensatn.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR Pfam; PF00501; AMP-binding; 4.
DR Pfam; PF00668; Condensation; 6.
DR Pfam; PF00550; PP-binding; 6.
DR SMART; SM00823; PKS_PP; 6.
DR SUPFAM; SSF47336; SSF47336; 6.
DR TIGRFAMs; TIGR01733; AA-adenyl-dom; 3.
DR PROSITE; PS00455; AMP_BINDING; 1.
DR PROSITE; PS50075; CARRIER; 6.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 5.
PE 2: Evidence at transcript level;
KW Ligase; Multifunctional enzyme; Phosphopantetheine; Phosphoprotein.
FT CHAIN 1..5386
FT /note="Nonribosomal peptide synthetase 2"
FT /id="PRO_0000444384"
FT DOMAIN 544..617
FT /note="Carrier 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258,
FT ECO:0000305|PubMed:15755917"
FT DOMAIN 1611..1688
FT /note="Carrier 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258,
FT ECO:0000305|PubMed:15755917"
FT DOMAIN 2652..2725
FT /note="Carrier 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258,
FT ECO:0000305|PubMed:15755917"
FT DOMAIN 3728..3805
FT /note="Carrier 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258,
FT ECO:0000305|PubMed:15755917"
FT DOMAIN 4281..4357
FT /note="Carrier 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258,
FT ECO:0000305|PubMed:15755917"
FT DOMAIN 4840..4913
FT /note="Carrier 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258,
FT ECO:0000305|PubMed:15755917"
FT REGION 45..435
FT /note="Adenylation 1"
FT /evidence="ECO:0000255, ECO:0000305|PubMed:15755917"
FT REGION 652..1059
FT /note="Condensation 1"
FT /evidence="ECO:0000255, ECO:0000305|PubMed:15755917"
FT REGION 1089..1482
FT /note="Adenylation 2"
FT /evidence="ECO:0000255, ECO:0000305|PubMed:15755917"
FT REGION 1731..2141
FT /note="Condensation 2"
FT /evidence="ECO:0000255, ECO:0000305|PubMed:15755917"
FT REGION 2166..2551
FT /note="Adenylation 3"
FT /evidence="ECO:0000255, ECO:0000305|PubMed:15755917"
FT REGION 2763..3174
FT /note="Condensation 3"
FT /evidence="ECO:0000255, ECO:0000305|PubMed:15755917"
FT REGION 3202..3603
FT /note="Adenylation 4"
FT /evidence="ECO:0000255, ECO:0000305|PubMed:15755917"
FT REGION 3846..4250
FT /note="Condensation 4"
FT /evidence="ECO:0000255, ECO:0000305|PubMed:15755917"
FT REGION 4391..4802
FT /note="Condensation 5"
FT /evidence="ECO:0000255, ECO:0000305|PubMed:15755917"
FT REGION 4821..4842
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 4952..5257
FT /note="Condensation 6"
FT /evidence="ECO:0000255, ECO:0000305|PubMed:15755917"
FT MOD_RES 578
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT MOD_RES 1648
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT MOD_RES 2686
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT MOD_RES 3765
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT MOD_RES 4318
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT MOD_RES 4874
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
SQ SEQUENCE 5386 AA; 600338 MW; DFEBAC685156E13A CRC64;
MSSATSPCEM AHKNRLNQTL SILNGQPLLL DGPDLLHQLV PRLHHDANAI DFLEHGSKRR
KFSYTTLHSL SDAFAARITE ILGKLESASS IIPVFLPQSP ELYVVLLAIL KAGKAFCPLN
LDTPTERLKF ILDDISADII ITFESYSEHI RTATNIHVVS ANRELSGCHD TFHHHSPHLS
PDNLAYVLYT SGSTGLPKAV SVSHRAVTQS LLAHDPHIPA FSRFLQFAAP TFDVSIFEIF
FPWFRGKTLV GCTRTQMLDD LPGTIASLDV DAAELTPTVV SSLLSGRSSV PGLKLLLTIG
EMLTQPVIDE FGGDATKESI LWAMYGPTEA AIHCTIWPQF STSDSTNTIG HPLDTVSAFI
LASSTGLHTS PIDILPIGQA GELAIGGPQV AKEYLHRPDL TRASFVEHPY YGRLYRTGDR
ARINEQGLLE CLGRVVAGQV KLRGQRIELG EIEQAIMKTR GCRAVTAMVI QDNLVAFCSG
RDGMSRGAVL TTCKHWLPAS MIPSDVFVID VMPQLPSGKV DRKSLEKAYL HSHPNGSSSS
LAISPKDPIG HSVWSVVSHH TTQNIGLETN LTSIGIDSLK SIRIASALRR KGYSLGAIEV
LSAATLADLI EVCRESKPVD FSSQDKETKP GAFIDTKNLQ LNGWRHNVAL ILPCTPLQEA
MLAETRSKPT AYCNWIEVEL SVAYTYEQIQ DALLFLAQEN EILRTGFCID SQHTTVFSQV
IWKELSLSQI QKVASFSNQY SMQSDHDFLR PFGVQIKTHC KLPRLLFQIH HALYDGWSLD
LLLRDLDHCL RGKQDLKRPS FREVVRYLDD DRRLNKTQNS TNYWKSLLGD YIPTTLPNYH
GKLVHNASIH RFFGQSSVSR HNLFECAHHS AINPQVYFQA ATAFVLSLYT GSSDVVLGNV
TSGRTIPVAG IEDIIGPCIA SLPFRIDFAD TYCVRDVLKR TQSTNRDSLR YSQLPLREIA
RAVNVKPGAR LFETLFVWQQ SMVEDEDDNA SLIAKVVDSA DELEFRITLE FEPVGDNILF
RSTFDAATVS EHQIQYLFRQ IDEVVEMFMV DADRHVSAIN QCFTTPSLSI ANPTPLEQRF
DHGPSHAVEK WAATDPHRTA IIFGHEVNGS IKVKDTMTYS MLNSRANQLA RLLAEHGVTN
DQLVCIIMEK SVNLYTCILA VLKLGCGYLP LVPDTPIDRV KTILNDAQIA VCMSELSLSA
TLRSHLSVDI IDFDLAALSD YCDRNLEIPY NGQHLAYAVF TSGSTGTPKG VLVTQDNLMS
NLHYLSTIYP FSADSRLLQA CSQAFDVSVF EIFFTWYVGI CLCSATKEHL FRDFEAAIDQ
LKVTHLSLTP TVAALVDPKN VPKVEFLVTA GEAVTEHVRR KWAGRGLYQG YGPSETTNIC
TVRVAVTPDD LINNIGSPFA NTSAFVLDPE SQDILPRGAV GELCFGGSQV FRGYLNRPEL
NAQKIIQHPT YGRIYRSGDM GILLPDDSIL STGRTDDQVK IRGQRVELGE VTSVILDHGA
VWDCVTLALE QSTNSKTLVS FWVPREDSSS RVESLEPSKF TTTISELFDL LSRRVPSYMV
PSHLIPISCL PMTPQAKIDK RFLQRLFSSC EEHTLNNATN SNSITETEEG ELLSQWERDV
SQLLIRMLAT SSDKLKRTSS FFNLGIDSVS AIRFCSELRK AGLGDYSVSE VLKHPSIASL
ASLKKLQSST TTTTTTMDKT LSVDASHIFT TNQLERIRST VDMNGVRATK ILPCTPLQEA
MISSGLSSPG QAYCNVMVFD VKGDLQQLQR CWKTVIQRHE IFRTSFVATD NPLYSFAQVI
VEGYGMGWHE DSIDSGLQLR VSKILFDLME ANKPPVYFSL NREEDSAKLL FCCHHALYDG
IAMSTLLAEV QELYHGRQLL PPVSYDVYLK RMLEQNLDEA DKYWSALLEG FEPTSFPSLT
GKRVREYEAF TSSSRRLSMS LDSVRNSCQN SSVSLLSVVH AAWAKLLHFY THESDICFGN
IVSGRSFPGE HLERLVAPCF NTLPVRVDFD FGKSNRALVD LMHNQSIESL AYQLSPLRRI
QKTTLKDGGR LFDSLVILQQ PNVPLDSSIW RLEQDSGDMD IPVVCEVVQD QSEDALRLLM
HYNNSLISET EASIVMETFD AALSSLIKFP DALSADTDMF PSNLWANYNT NFKRLESDSK
FLHSGFERTA LLHPDRIALD FWHSQGKKTT WSFEQLNREA NQIAHALIRA GAWPDQVIPI
HISKSPIYYA SILGVLKSGA AFAPVHPDLP EARKQLMFKD LKPKIILCDD GSLLPEDLPD
VTVLITQSMS SDDVSNPIIE DLKDTNLAYC LFTSGSTGVP KAVSMEHCAP IQTIESSRTI
IPWNPQSRLL QYAAVTFDMC YYDCFLSWTF GFALCAAEQS DLLNDLSGVI KTLEADLLDL
TPSVAETLKR ADVPNVKWLY CIGEAMSSSV VKEWEGACVN SYGPTEAAFC TTITPLSKDE
STSIIGKPFP TTSFAVFSEG SQTPLPALSI GELYIGGAQL ARGYWGRANL TNDRFVSRCG
QRFYKSGDMV RMLSDGNFEF MGRLDDQVKI RGLRVELGEI NSILAELDPD LLSVTTQILL
KGESSKEQLV SFMVLRQSIQ ESDIPTLQRK LKKLASARLP SYMVPQFFLV VDEIPKSMAG
KIDKKALKHQ WSKTESEVRN ILAHISKTPT ENISPLTSIY QLGLDSISAV QIASALRSQG
YTIKATDVLK HTTCNDLAEH LDQISTSEAP ESSPFDFHGF ESRHRMQILR DHGIQDGNVA
AVRPCTPLQN GMVSQFLAKE GAVYMNYLRL QLEPKVDLEK LKAAWSSTME RHSLLRTGFA
HVNDPLFPFA MIEYTQVSVT LPWSAIREQK KSQSSNAWLQ RIRAQSLKEL YVPPWALRFV
ERNDQSFLDL AIFHALFDAQ SLQNIFTDVT AFYKGLSLPP VPSLNEVVSH VIQSYKQDNS
SGKEFWVELG KTANPSRFPN LAPLKCDPKP PIVCTRRSAK SLIDLENGCR QANTTMPAVG
MASWLSLLSS YTGESSVTCG VVLSGRSSDA TAHANFPCIN TVPLAFTVAN DTTKMLESIT
VLNAGIQEHQ FKPLKEIQAL MGFPNESLFD SIFAYQKLAN NKDTSDLWTV VDENATIEYP
VSIELEPKEE RLEYRLTYFP HIIPREQASL ILAQLDHLME SLIFHSQIPL AKTDYSQHLY
SITPAKEDEL PSDMKLLHEL VEKTAQEHPQ RIAFEFVSKE SSGKRPVRKW TYRELDQEGN
KIAQLLAAHN VKQNSLVGVC FDKCPEASFA MLGILKAGCA FVAIDPGAPA ARQTFIIEDS
DAQAVLSMSS QSAQFNAIAK VPVLNLDEVE WCSLSGQKLL QNSVIDPQDR SYCLYTSGTT
GTPKGCELTH ENAVQALLAF QRLFAGHWDV DSRWLQFASF HFDVSVLEQY WSWSVGICVV
SAPRDLIFED LAGSIRDLNI THIDLTPSLA QILHPDDVPS LCKGVFITGG ESLKQEILDV
WGPKGVIYNG YGPTEATIGC TMYPRVPANG KPSNIGPQFD NVGSLVLRPG SDVPVLRGGV
GELCVSGKLV GKGYLNRPDL TTERFPYLNR FSQRVYRTGD VVRILHDGTF HFLGRADDQV
KLRGQRLEVA EINSVIKQSD SDISDVATLV LKHPKQQKEQ LVSFVVCGKA LKAQPEVLLG
EVGGIASAKQ ACNDKLPPYM LPTHFVPLTS MPLNVNNKAD GKALKKMYES LSSTDLQKLS
ATSLSRDEQW SKQEEKLRDV MLEALGADHE SMSKNTSFYE LGMDSISVIG VTQSLKQSGF
TKVTTSMILQ CPTIRRLAKS LAANSAMSNV RGSILAAQQS INAVNHRHRR KIAKRLSVKP
SMIESLAPCT PLQQGMIARS MENGNGLYFN TFRFRLNMDV DEGKLRHAWE TMYNSTQILR
TVFVNTEEGY LQAVLGGIPF NGFIQTSTQD DDLISHMAQL HKDWLSLNDV DFRQPFQVHL
VSAQKQKQLI VHIFHGLYDG NSIGLLLQGV WNSYERRDSM PDAPSFHTAL AHGPLRIPDD
AKSFWKDLIL ARTSSLPTLF DNSSQDAVVI ARTMRAPANF DLIRRQLNVT AQAVVQACWF
SVLHRHVKGD VATGIIVSGR SIEFEGADRV IGPMFNTIPY HHRAQRSESW SSIIERVHDF
NIQAHPFQHT PLRDIMKWCK RSPSNPLFDN LFVYQVPQDN QEWAKNDLWT LLDDEAIADY
PLAFEVEHRG GTELKLTLVT QGHVANDQIA AKLLDMFEEA LDQAINDPSA VLELPADVDG
AVENNTAIRS KLDDNNDISD FEWSDNAIAI REEIANLTAN EMESISETTS IFELGLDSID
AIKLSSKLKK RGMELSVSGI MRGLTIEKMA QNMSMKNTQT TEAAPHFDLD AHKTKLAKCL
YHQGFSADDI EEILPLTPLQ EAMVAEMIAS EYTRYFNHDV LKLSPDTDIS KFQKAWTTVV
MGSPILRTGF VEVDNPDIDL SFAQIIHRQP HDFYSHMSFG SRPDFASIFK DLRNDAIQRP
LSTPLFHLTF IDTPDQSYLV LSIAHALYDG WSLSLLHSDV HRAYQNEFEA RPSYQPSLAE
IIKTSGPDAA GFWQDYLSGA NGNTFPRRTL EPDEKSSTVH RHQENSKIAL ELIQSFARKN
NVSLQTVGQT VFALVTASFT RSLDVTFGSV LSGRDEEETS QLMFPTMNTV AIRTILHGKS
IELLRYVQDN FANIKQWQHY PLRKAMSQAR LDGRLFDSLF IYQKRLEQQQ NEGERLYTSV
GGHSDVEYAV CVEMEVVKEA LIWRCAVKDD VFDLEETRQL LKRMDDVLIH LMERAEAPVI
DMTAEGTSVC GLPAFEEAEM HTGSGHVESG EDDGQDTPST ETTNRIRKIL AAVSKTPEEE
MTNDMTIFHM GLDSISAIKV SSLLRKQGVV LSVGEMLQAG SVEKMAKLAD ARATEPSKDD
AIDSASLGEI LKELNEAEVF KRAGVDVDNV VQMLPVTAGQ LYMLSMWLNT NGSNFYPEFS
YEFEADVAFE DLKKAWQALV TTNPILRTCF VSVGNHQVSY VQLVLRDIDI AITNVTEYGE
EEIRNCIRKA TTQQPWARLL VSRNSHSWTL RLKIHHALYD GISLPLLMQQ FEDLCNGSVL
NASRNDILAD FISSTSSLSS SPQRRQFWET HLLSRTAPTQ LKQPSHTPTT RLEIFRPSLT
PIQTLDTTAR HHGISPHALF LAIYAKLHSR LPHSTTTDDN IVLGIYLANR SLSCTPELPG
AAIPTLNLVP LRVSTPQSRS VVESAKQVQA HLRHLNDATL ATTSLVEIER WTGVKIDVFV
NFLVDIDDQG ARPKHAHQRV KISPTLRQQY YCSSFSRTSS VVQPEFMDFQ ALRNEHVNGV
YLHAIDIEAT VREGYLDVGI FAPGDMISLE QGEQLMDGLK GEVEGL
