NPS3A_HUMAN
ID NPS3A_HUMAN Reviewed; 247 AA.
AC Q9UFN0; A6NM55; Q5VX32; Q9BRV7; Q9H843; Q9P083;
DT 26-SEP-2001, integrated into UniProtKB/Swiss-Prot.
DT 26-SEP-2001, sequence version 2.
DT 03-AUG-2022, entry version 159.
DE RecName: Full=Protein NipSnap homolog 3A;
DE Short=NipSnap3A;
DE AltName: Full=Protein NipSnap homolog 4;
DE Short=NipSnap4;
DE AltName: Full=Target for Salmonella secreted protein C;
DE Short=TassC;
GN Name=NIPSNAP3A; Synonyms=NIPSNAP4; ORFNames=HSPC299;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT GLN-100.
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Umbilical cord blood;
RA Ye M., Zhang Q.-H., Zhou J., Shen Y., Wu X.-Y., Guan Z.Q., Wang L.,
RA Fan H.-Y., Mao Y.-F., Dai M., Huang Q.-H., Chen S.-J., Chen Z.;
RT "Human partial CDS from CD34+ stem cells.";
RL Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15164053; DOI=10.1038/nature02465;
RA Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L.,
RA Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R.,
RA Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S.,
RA Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K.,
RA Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y.,
RA Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C.,
RA Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E.,
RA Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M.,
RA Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J.,
RA Frankish A., Frankland J.A., French L., Fricker D.G., Garner P.,
RA Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S.,
RA Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E.,
RA Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D.,
RA Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E.,
RA Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K.,
RA Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S.,
RA Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J.,
RA Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E.,
RA McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V.,
RA Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S.,
RA Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K.,
RA Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J.,
RA Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M.,
RA West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L.,
RA Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M.,
RA Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J.,
RA Dunham I.;
RT "DNA sequence and analysis of human chromosome 9.";
RL Nature 429:369-374(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Bone marrow;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND INTERACTION WITH SALMONELLA
RP SPIC.
RX PubMed=12427096; DOI=10.1046/j.1462-5822.2002.00225.x;
RA Lee A.H., Zareei M.P., Daefler S.;
RT "Identification of a NIPSNAP homologue as host cell target for Salmonella
RT virulence protein SpiC.";
RL Cell. Microbiol. 4:739-750(2002).
RN [8]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-48 AND LYS-166, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19608861; DOI=10.1126/science.1175371;
RA Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C.,
RA Olsen J.V., Mann M.;
RT "Lysine acetylation targets protein complexes and co-regulates major
RT cellular functions.";
RL Science 325:834-840(2009).
RN [9]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [10]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA Ye M., Zou H.;
RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT phosphoproteome.";
RL J. Proteomics 96:253-262(2014).
RN [11]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=25944712; DOI=10.1002/pmic.201400617;
RA Vaca Jacome A.S., Rabilloud T., Schaeffer-Reiss C., Rompais M., Ayoub D.,
RA Lane L., Bairoch A., Van Dorsselaer A., Carapito C.;
RT "N-terminome analysis of the human mitochondrial proteome.";
RL Proteomics 15:2519-2524(2015).
CC -!- SUBUNIT: Interacts with the Salmonella typhimurium virulence protein
CC spiC. {ECO:0000269|PubMed:12427096}.
CC -!- INTERACTION:
CC Q9UFN0; Q8NHQ1: CEP70; NbExp=3; IntAct=EBI-716291, EBI-739624;
CC Q9UFN0; Q9H3D4: TP63; NbExp=2; IntAct=EBI-716291, EBI-2337775;
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000269|PubMed:12427096}.
CC Note=May be part of some vesicular structure distinct from lysosomal
CC vesicles.
CC -!- TISSUE SPECIFICITY: Ubiquitous. Highly expressed in liver, kidney and
CC muscle. Expressed at intermediate level in brain, heart, colon, thymus,
CC kidney, small intestine, placenta, lung, leukocytes and spleen.
CC {ECO:0000269|PubMed:12427096}.
CC -!- SIMILARITY: Belongs to the NipSnap family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF28977.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AK024015; BAB14777.1; -; mRNA.
DR EMBL; AL117557; CAB55992.2; -; mRNA.
DR EMBL; AF161417; AAF28977.1; ALT_INIT; mRNA.
DR EMBL; AL359846; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471105; EAW58990.1; -; Genomic_DNA.
DR EMBL; BC005935; AAH05935.1; -; mRNA.
DR CCDS; CCDS6760.1; -.
DR RefSeq; NP_001316499.1; NM_001329570.1.
DR RefSeq; NP_056284.1; NM_015469.2.
DR AlphaFoldDB; Q9UFN0; -.
DR SMR; Q9UFN0; -.
DR BioGRID; 117432; 92.
DR IntAct; Q9UFN0; 25.
DR MINT; Q9UFN0; -.
DR STRING; 9606.ENSP00000363899; -.
DR ChEMBL; CHEMBL3817722; -.
DR iPTMnet; Q9UFN0; -.
DR MetOSite; Q9UFN0; -.
DR PhosphoSitePlus; Q9UFN0; -.
DR BioMuta; NIPSNAP3A; -.
DR DMDM; 18203499; -.
DR OGP; Q9UFN0; -.
DR UCD-2DPAGE; Q9UFN0; -.
DR EPD; Q9UFN0; -.
DR jPOST; Q9UFN0; -.
DR MassIVE; Q9UFN0; -.
DR MaxQB; Q9UFN0; -.
DR PaxDb; Q9UFN0; -.
DR PeptideAtlas; Q9UFN0; -.
DR PRIDE; Q9UFN0; -.
DR ProteomicsDB; 84191; -.
DR Antibodypedia; 29247; 121 antibodies from 27 providers.
DR DNASU; 25934; -.
DR Ensembl; ENST00000374767.5; ENSP00000363899.4; ENSG00000136783.10.
DR GeneID; 25934; -.
DR KEGG; hsa:25934; -.
DR MANE-Select; ENST00000374767.5; ENSP00000363899.4; NM_015469.3; NP_056284.1.
DR UCSC; uc004bch.1; human.
DR CTD; 25934; -.
DR DisGeNET; 25934; -.
DR GeneCards; NIPSNAP3A; -.
DR HGNC; HGNC:23619; NIPSNAP3A.
DR HPA; ENSG00000136783; Low tissue specificity.
DR MIM; 608871; gene.
DR neXtProt; NX_Q9UFN0; -.
DR OpenTargets; ENSG00000136783; -.
DR PharmGKB; PA134954501; -.
DR VEuPathDB; HostDB:ENSG00000136783; -.
DR eggNOG; KOG2883; Eukaryota.
DR GeneTree; ENSGT00950000183018; -.
DR HOGENOM; CLU_085919_0_1_1; -.
DR InParanoid; Q9UFN0; -.
DR OMA; AVHAHIN; -.
DR OrthoDB; 1141024at2759; -.
DR PhylomeDB; Q9UFN0; -.
DR TreeFam; TF314501; -.
DR PathwayCommons; Q9UFN0; -.
DR SignaLink; Q9UFN0; -.
DR BioGRID-ORCS; 25934; 6 hits in 1044 CRISPR screens.
DR ChiTaRS; NIPSNAP3A; human.
DR GenomeRNAi; 25934; -.
DR Pharos; Q9UFN0; Tdark.
DR PRO; PR:Q9UFN0; -.
DR Proteomes; UP000005640; Chromosome 9.
DR RNAct; Q9UFN0; protein.
DR Bgee; ENSG00000136783; Expressed in adrenal tissue and 182 other tissues.
DR Genevisible; Q9UFN0; HS.
DR GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; HDA:UniProtKB.
DR InterPro; IPR011008; Dimeric_a/b-barrel.
DR InterPro; IPR012577; NIPSNAP.
DR Pfam; PF07978; NIPSNAP; 2.
DR SUPFAM; SSF54909; SSF54909; 2.
PE 1: Evidence at protein level;
KW Acetylation; Cytoplasm; Reference proteome.
FT CHAIN 1..247
FT /note="Protein NipSnap homolog 3A"
FT /id="PRO_0000221152"
FT MOD_RES 48
FT /note="N6-acetyllysine"
FT /evidence="ECO:0007744|PubMed:19608861"
FT MOD_RES 166
FT /note="N6-acetyllysine"
FT /evidence="ECO:0007744|PubMed:19608861"
FT VARIANT 100
FT /note="R -> Q (in dbSNP:rs2274870)"
FT /evidence="ECO:0000269|PubMed:14702039"
FT /id="VAR_020442"
FT CONFLICT 108
FT /note="E -> G (in Ref. 3; AAF28977)"
FT /evidence="ECO:0000305"
FT CONFLICT 118
FT /note="L -> S (in Ref. 3; AAF28977)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 247 AA; 28467 MW; DA668E2AD42BA1AA CRC64;
MLVLRSALTR ALASRTLAPQ MCSSFATGPR QYDGIFYEFR SYYLKPSKMN EFLENFEKNA
HLRTAHSELV GYWSVEFGGR MNTVFHIWKY DNFAHRTEVR KALAKDKEWQ EQFLIPNLAL
IDKQESEITY LVPWCKLEKP PKEGVYELAT FQMKPGGPAL WGDAFKRAVH AHVNLGYTKL
VGVFHTEYGA LNRVHVLWWN ESADSRAAGR HKSHEDPRVV AAVRESVNYL VSQQNMLLIP
TSFSPLK
