NPS4B_DANRE
ID NPS4B_DANRE Reviewed; 844 AA.
AC E7FFX1; E9QDD8;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 08-MAR-2011, sequence version 1.
DT 03-AUG-2022, entry version 76.
DE RecName: Full=Neuronal PAS domain-containing protein 4B {ECO:0000305};
GN Name=npas4b {ECO:0000312|ZFIN:ZDB-GENE-091117-33};
GN ORFNames=si:dkey-17e16.11 {ECO:0000312|ZFIN:ZDB-GENE-091117-33};
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
CC -!- FUNCTION: Transcription factor expressed in neurons of the brain that
CC regulates the excitatory-inhibitory balance within neural circuits and
CC is required for contextual memory in the hippocampus. Plays a key role
CC in the structural and functional plasticity of neurons. Acts as an
CC early-response transcription factor in both excitatory and inhibitory
CC neurons, where it induces distinct but overlapping sets of late-
CC response genes in these two types of neurons, allowing the synapses
CC that form on inhibitory and excitatory neurons to be modified by
CC neuronal activity in a manner specific to their function within a
CC circuit, thereby facilitating appropriate circuit responses to sensory
CC experience. {ECO:0000250|UniProtKB:Q8BGD7}.
CC -!- SUBUNIT: Efficient DNA binding requires dimerization with another bHLH
CC protein. {ECO:0000250|UniProtKB:Q8BGD7}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q8BGD7,
CC ECO:0000255|PROSITE-ProRule:PRU00981}.
CC -!- CAUTION: Contains a degenerate basic motif not likely to bind DNA.
CC {ECO:0000255|PROSITE-ProRule:PRU00981}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CR388166; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_009293710.2; XM_009295435.2.
DR AlphaFoldDB; E7FFX1; -.
DR STRING; 7955.ENSDARP00000105317; -.
DR PaxDb; E7FFX1; -.
DR GeneID; 100534657; -.
DR KEGG; dre:100534657; -.
DR CTD; 100534657; -.
DR ZFIN; ZDB-GENE-091117-33; npas4b.
DR eggNOG; ENOG502QRXX; Eukaryota.
DR HOGENOM; CLU_013890_0_0_1; -.
DR InParanoid; E7FFX1; -.
DR OMA; QVETADH; -.
DR OrthoDB; 219290at2759; -.
DR PhylomeDB; E7FFX1; -.
DR TreeFam; TF319684; -.
DR PRO; PR:E7FFX1; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Unplaced.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0098794; C:postsynapse; IEA:GOC.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISS:UniProtKB.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; ISS:UniProtKB.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0001568; P:blood vessel development; IGI:ZFIN.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0060079; P:excitatory postsynaptic potential; ISS:UniProtKB.
DR GO; GO:0060080; P:inhibitory postsynaptic potential; ISS:UniProtKB.
DR GO; GO:1904862; P:inhibitory synapse assembly; ISS:UniProtKB.
DR GO; GO:0007612; P:learning; ISS:UniProtKB.
DR GO; GO:0007616; P:long-term memory; ISS:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; ISS:UniProtKB.
DR GO; GO:0048167; P:regulation of synaptic plasticity; ISS:UniProtKB.
DR GO; GO:0032228; P:regulation of synaptic transmission, GABAergic; ISS:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0007614; P:short-term memory; ISS:UniProtKB.
DR GO; GO:0035176; P:social behavior; ISS:UniProtKB.
DR CDD; cd00130; PAS; 2.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR000014; PAS.
DR InterPro; IPR035965; PAS-like_dom_sf.
DR InterPro; IPR020891; UPF0758_CS.
DR SMART; SM00091; PAS; 2.
DR SUPFAM; SSF55785; SSF55785; 2.
DR PROSITE; PS50888; BHLH; 1.
DR PROSITE; PS50112; PAS; 1.
PE 3: Inferred from homology;
KW Activator; Differentiation; DNA-binding; Neurogenesis; Nucleus;
KW Reference proteome; Repeat; Transcription; Transcription regulation.
FT CHAIN 1..844
FT /note="Neuronal PAS domain-containing protein 4B"
FT /id="PRO_0000438133"
FT DOMAIN 61..114
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT DOMAIN 132..190
FT /note="PAS 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00140"
FT DOMAIN 294..343
FT /note="PAS 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00140"
FT REGION 61..74
FT /note="Basic motif; degenerate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 75..114
FT /note="Helix-loop-helix motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 410..432
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 444..479
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 702..725
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 757..784
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 444..461
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 704..718
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 759..775
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 844 AA; 93724 MW; AA7AAC93AC7CF0DC CRC64;
MFSSESRPTV KLPDKWAKKR CFFQLLSSLS TTLKKLCSSS SHCSGFHLII RPFQRFFGLE
KMYRSTKGAS KARRDQINAE IRSLKELLPI SDADKARLSY LHIMSLACIY TRKSVFFSQA
AAGHGMSGSL LSLPELSDLL HTLPGFLLVL TSEGKLLYLS DNVAEHLGHS MVDLVAQSDS
VYDIIDPVDH FIMRGNLVPI TTPDTDRLFR CRFSTSKFVR RQGSGNKQAI VRARCLPPPY
HASPYWTSNP VWVCFCSPLE ASMPQLSTSR NPLPTPPAEQ SFLLSCFQSQ HSRDMRIHTV
QDSVSVYLGY DIETLRSRSW YSLIHPRDLS HASAQHCTLL HNGGERQVEM VVQVEAADHS
WIWLYIVLQL ETGEYPINSH NYVISESEAW SVRQQLHSEQ NQLALLYQES RQSSDPLSSP
DQVFTPSSSG LSSQSFDFSF ITSGRSSSEE LPGTSAPSSM TFDPLEGEEI DPQSHGGGHQ
MWRSAMTIAP EHLSNINLST VPQSQVAPPP LPPFKAPPKR QRSEEFICTP PYTPRLSGGS
FIFNDETLRP SDHSKSAKMR QSITSATNIG PAQPCRKRLY ETLPPTPDSP GSDECILMAL
PEIRGPLYVD VPHLPFHAPP EGLLTPEASP TKKPCLSFFP REDETERERM EISLLAQYIS
TLAEGFCHNH PQGASAPPHH ANSSLAHIDV LMFEEKAVDD IPLPNLPSPS PVPPSPYSSV
PSYSQCRSSP VQEEAAVTLI GVNHLCSVQL THCNRMTEGG LQDGERPDED MEMMSSQRSS
EAPALTPALP CAQSLLEELV TMEPVFGAAV PMTPADRQQD ELYQLPHQGG PQMFYQDGTG
DHMF
