NPSR1_MOUSE
ID NPSR1_MOUSE Reviewed; 371 AA.
AC Q8BZP8; Q80T64; Q8BYA1;
DT 08-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=Neuropeptide S receptor;
DE AltName: Full=G-protein coupled receptor 154;
DE AltName: Full=G-protein coupled receptor PGR14;
GN Name=Npsr1; Synonyms=Gpr154, Pgr14;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Diencephalon, and Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 176-222.
RX PubMed=12679517; DOI=10.1073/pnas.0230374100;
RA Vassilatis D.K., Hohmann J.G., Zeng H., Li F., Ranchalis J.E.,
RA Mortrud M.T., Brown A., Rodriguez S.S., Weller J.R., Wright A.C.,
RA Bergmann J.E., Gaitanaris G.A.;
RT "The G protein-coupled receptor repertoires of human and mouse.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:4903-4908(2003).
RN [3]
RP INDUCTION.
RX PubMed=15073379; DOI=10.1126/science.1090010;
RA Laitinen T., Polvi A., Rydman P., Vendelin J., Pulkkinen V.,
RA Salmikangas P., Maekelae S., Rehn M., Pirskanen A., Rautanen A.,
RA Zucchelli M., Gullsten H., Leino M., Alenius H., Petaeys T., Haahtela T.,
RA Laitinen A., Laprise C., Hudson T.J., Laitinen L.A., Kere J.;
RT "Characterization of a common susceptibility locus for asthma-related
RT traits.";
RL Science 304:300-304(2004).
CC -!- FUNCTION: G-protein coupled receptor for neuropeptide S (NPS). Promotes
CC mobilization of intracellular Ca(2+) stores. Inhibits cell growth in
CC response to NPS binding. Involved in pathogenesis of asthma and other
CC IgE-mediated diseases. {ECO:0000250|UniProtKB:Q6W5P4}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q6W5P4};
CC Multi-pass membrane protein {ECO:0000250|UniProtKB:Q6W5P4}.
CC -!- INDUCTION: Increased expression in lung after ovalbumin induction in a
CC mouse model of ovalbumin-induced lung inflammation.
CC {ECO:0000269|PubMed:15073379}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC Vasopressin/oxytocin receptor subfamily. {ECO:0000255|PROSITE-
CC ProRule:PRU00521}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC30949.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AK033957; BAC28526.1; -; mRNA.
DR EMBL; AK041451; BAC30949.1; ALT_INIT; mRNA.
DR EMBL; AY255574; AAO85086.1; -; mRNA.
DR CCDS; CCDS22930.1; -.
DR RefSeq; NP_783609.1; NM_175678.3.
DR RefSeq; XP_017168906.1; XM_017313417.1.
DR AlphaFoldDB; Q8BZP8; -.
DR SMR; Q8BZP8; -.
DR STRING; 10090.ENSMUSP00000056432; -.
DR BindingDB; Q8BZP8; -.
DR ChEMBL; CHEMBL5497; -.
DR GuidetoPHARMACOLOGY; 302; -.
DR GlyGen; Q8BZP8; 2 sites.
DR PhosphoSitePlus; Q8BZP8; -.
DR PaxDb; Q8BZP8; -.
DR PRIDE; Q8BZP8; -.
DR Antibodypedia; 1941; 198 antibodies from 27 providers.
DR DNASU; 319239; -.
DR Ensembl; ENSMUST00000059650; ENSMUSP00000056432; ENSMUSG00000043659.
DR GeneID; 319239; -.
DR KEGG; mmu:319239; -.
DR UCSC; uc009oox.1; mouse.
DR CTD; 387129; -.
DR MGI; MGI:2441738; Npsr1.
DR VEuPathDB; HostDB:ENSMUSG00000043659; -.
DR eggNOG; KOG3184; Eukaryota.
DR eggNOG; KOG3656; Eukaryota.
DR GeneTree; ENSGT00940000155094; -.
DR HOGENOM; CLU_009579_15_0_1; -.
DR InParanoid; Q8BZP8; -.
DR OMA; WIPYMTV; -.
DR OrthoDB; 1034048at2759; -.
DR PhylomeDB; Q8BZP8; -.
DR TreeFam; TF106499; -.
DR Reactome; R-MMU-375276; Peptide ligand-binding receptors.
DR Reactome; R-MMU-416476; G alpha (q) signalling events.
DR Reactome; R-MMU-418555; G alpha (s) signalling events.
DR BioGRID-ORCS; 319239; 2 hits in 74 CRISPR screens.
DR ChiTaRS; Npsr1; mouse.
DR PRO; PR:Q8BZP8; -.
DR Proteomes; UP000000589; Chromosome 9.
DR RNAct; Q8BZP8; protein.
DR Bgee; ENSMUSG00000043659; Expressed in dentate gyrus of hippocampal formation granule cell and 13 other tissues.
DR ExpressionAtlas; Q8BZP8; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0005887; C:integral component of plasma membrane; ISS:UniProtKB.
DR GO; GO:0008188; F:neuropeptide receptor activity; ISS:UniProtKB.
DR GO; GO:0005000; F:vasopressin receptor activity; IEA:InterPro.
DR GO; GO:0042755; P:eating behavior; ISO:MGI.
DR GO; GO:2000293; P:negative regulation of defecation; ISO:MGI.
DR GO; GO:1903999; P:negative regulation of eating behavior; ISO:MGI.
DR GO; GO:0007218; P:neuropeptide signaling pathway; ISS:UniProtKB.
DR GO; GO:0070374; P:positive regulation of ERK1 and ERK2 cascade; ISO:MGI.
DR GO; GO:0051281; P:positive regulation of release of sequestered calcium ion into cytosol; ISS:UniProtKB.
DR GO; GO:0060013; P:righting reflex; ISO:MGI.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR027294; NPS_rcpt.
DR InterPro; IPR001817; Vasoprsn_rcpt.
DR PANTHER; PTHR24244:SF2; PTHR24244:SF2; 1.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PRINTS; PR00896; VASOPRESSINR.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Disulfide bond; G-protein coupled receptor; Glycoprotein;
KW Membrane; Receptor; Reference proteome; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..371
FT /note="Neuropeptide S receptor"
FT /id="PRO_0000069641"
FT TOPO_DOM 1..52
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 53..73
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 74..82
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 83..103
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 104..123
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 124..144
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 145..164
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 165..185
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 186..212
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 213..233
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 234..275
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 276..296
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 297..312
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 313..333
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 334..371
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 4
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 13
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 121..197
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
SQ SEQUENCE 371 AA; 42439 MW; FBAF7B45BEE5F844 CRC64;
MPANLTEGSF HANQTVPMLD SSPVACTEIV TFTEALVAEE WGSFYSSFKT EQLITLWVLF
VVTIVGNSVV LFSTCRRKRK SRMTFFVTQL AITDSFTGLI NILTDIIWRF TGDFMAPDLV
CRVVRYLQVV LLYASTYVLV SLSIDRYHAI VYPMKFLQGE KQAKVLIGIA WSLSFLFSIP
TLIIFGKRTL SNGEVQCWAL WPDDSYWTPY MTIVAFLVYF IPLAIISVIY GLVIRTIWMK
SKTHETVISN CSDGKLCCSY NRGLISKAKI KAIKYSIVII LAFICCWSPY FLFDILDNFN
VLPDTKERFY ASVIIQNLPA LNSAINPLIY CIFSSSICSP CKMQRSQDSR MTYRERSERH
EMQILSKPEF I
