NPT1_MOUSE
ID NPT1_MOUSE Reviewed; 465 AA.
AC Q61983; A9Z1Z4; Q91YA1;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Sodium-dependent phosphate transport protein 1;
DE AltName: Full=Na(+)/PI cotransporter 1;
DE AltName: Full=Renal Na(+)-dependent phosphate cotransporter 1;
DE AltName: Full=Renal sodium-dependent phosphate transport protein 1;
DE Short=Renal sodium-phosphate transport protein 1;
DE AltName: Full=Sodium/phosphate cotransporter 1;
DE AltName: Full=Solute carrier family 17 member 1;
GN Name=Slc17a1; Synonyms=Npt1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Kidney;
RX PubMed=7611445; DOI=10.1152/ajprenal.1995.268.6.f1038;
RA Chong S.S., Kozak C.A., Liu L., Kristjansson K., Dunn S.T., Bourdeau J.E.,
RA Hughes M.R.;
RT "Cloning, genetic mapping, and expression analysis of a mouse renal sodium-
RT dependent phosphate cotransporter.";
RL Am. J. Physiol. 268:F1038-F1045(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-148.
RC STRAIN=129/SvJ;
RX PubMed=11704559; DOI=10.1152/ajprenal.0092.2001;
RA Soumounou Y., Gauthier C., Tenenhouse H.S.;
RT "Murine and human type I Na-phosphate cotransporter genes: structure and
RT promoter activity.";
RL Am. J. Physiol. 281:F1082-F1091(2001).
RN [4]
RP INTERACTION WITH PDZK1.
RX PubMed=11099500; DOI=10.1074/jbc.m008745200;
RA Gisler S.M., Stagljar I., Traebert M., Bacic D., Biber J., Murer H.;
RT "Interaction of the type IIa Na/Pi-cotransporter with PDZ proteins.";
RL J. Biol. Chem. 276:9206-9213(2001).
CC -!- FUNCTION: Important for the resorption of phosphate by the kidney. May
CC be involved in actively transporting phosphate into cells via Na(+)
CC cotransport in the renal brush border membrane. Plays a role in urate
CC transport in the kidney. {ECO:0000250|UniProtKB:Q14916}.
CC -!- SUBUNIT: Interacts with PDZK1. {ECO:0000269|PubMed:11099500}.
CC -!- SUBCELLULAR LOCATION: Apical cell membrane
CC {ECO:0000250|UniProtKB:Q14916}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Kidney.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Sodium/anion
CC cotransporter family. {ECO:0000305}.
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DR EMBL; X77241; CAA54459.1; -; mRNA.
DR EMBL; AL590388; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL606464; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AF361768; AAK97542.1; -; Genomic_DNA.
DR EMBL; AF361764; AAK97542.1; JOINED; Genomic_DNA.
DR EMBL; AF361766; AAK97542.1; JOINED; Genomic_DNA.
DR CCDS; CCDS26372.1; -.
DR PIR; S69915; S69915.
DR RefSeq; NP_001164109.1; NM_001170638.1.
DR RefSeq; NP_033224.3; NM_009198.3.
DR AlphaFoldDB; Q61983; -.
DR SMR; Q61983; -.
DR IntAct; Q61983; 4.
DR MINT; Q61983; -.
DR STRING; 10090.ENSMUSP00000006785; -.
DR TCDB; 2.A.1.14.6; the major facilitator superfamily (mfs).
DR GlyGen; Q61983; 3 sites.
DR iPTMnet; Q61983; -.
DR PhosphoSitePlus; Q61983; -.
DR jPOST; Q61983; -.
DR PaxDb; Q61983; -.
DR PRIDE; Q61983; -.
DR ProteomicsDB; 293955; -.
DR Antibodypedia; 25435; 77 antibodies from 21 providers.
DR DNASU; 20504; -.
DR Ensembl; ENSMUST00000006785; ENSMUSP00000006785; ENSMUSG00000021335.
DR Ensembl; ENSMUST00000110413; ENSMUSP00000106043; ENSMUSG00000021335.
DR GeneID; 20504; -.
DR KEGG; mmu:20504; -.
DR UCSC; uc007pvf.2; mouse.
DR CTD; 6568; -.
DR MGI; MGI:103209; Slc17a1.
DR VEuPathDB; HostDB:ENSMUSG00000021335; -.
DR eggNOG; KOG2532; Eukaryota.
DR GeneTree; ENSGT00940000162346; -.
DR HOGENOM; CLU_001265_5_0_1; -.
DR InParanoid; Q61983; -.
DR OMA; MRGTWAF; -.
DR OrthoDB; 577796at2759; -.
DR PhylomeDB; Q61983; -.
DR TreeFam; TF313535; -.
DR Reactome; R-MMU-428643; Organic anion transporters.
DR BioGRID-ORCS; 20504; 5 hits in 72 CRISPR screens.
DR ChiTaRS; Slc17a1; mouse.
DR PRO; PR:Q61983; -.
DR Proteomes; UP000000589; Chromosome 13.
DR RNAct; Q61983; protein.
DR Bgee; ENSMUSG00000021335; Expressed in right kidney and 30 other tissues.
DR ExpressionAtlas; Q61983; baseline and differential.
DR Genevisible; Q61983; MM.
DR GO; GO:0016324; C:apical plasma membrane; ISS:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005764; C:lysosome; IBA:GO_Central.
DR GO; GO:0015114; F:phosphate ion transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0015136; F:sialic acid transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0006820; P:anion transport; IBA:GO_Central.
DR GO; GO:0015739; P:sialic acid transport; IBA:GO_Central.
DR GO; GO:0006814; P:sodium ion transport; IEA:UniProtKB-KW.
DR GO; GO:0046415; P:urate metabolic process; ISO:MGI.
DR GO; GO:0015747; P:urate transport; ISS:UniProtKB.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR004745; Pi_cotranspt.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00894; 2A0114euk; 1.
DR PROSITE; PS50850; MFS; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Glycoprotein; Ion transport; Membrane; Reference proteome;
KW Sodium; Sodium transport; Symport; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..465
FT /note="Sodium-dependent phosphate transport protein 1"
FT /id="PRO_0000220937"
FT TRANSMEM 79..99
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 117..137
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 176..196
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 199..219
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 260..280
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 304..324
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 337..356
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 363..383
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 399..419
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 429..449
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CARBOHYD 39
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 47
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 56
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 123
FT /note="L -> I (in Ref. 1; CAA54459 and 3; AAK97542)"
FT /evidence="ECO:0000305"
FT CONFLICT 431
FT /note="F -> S (in Ref. 1; CAA54459)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 465 AA; 51649 MW; F60BF9F9C04F3A2D CRC64;
MENQCLPKKV PGFCSFRYGL AILLHFCNIA IMAQRVCLNL TMVAMVNNTG SPHLSNESVV
EMLDNVKNPV YSWSPDIQGL ILSSVFFGMV VVQAPVGYLS GIYPMKRIIG SSLFLSSLMS
LLLPPAAQVG AALVIVCRVL QGIAQGTVST GQHEIWVKWA PPLERGRLTS MTLSGFVMGP
FIVLLVSGFI CDLLGWPMVF YIFGIVGCVL SLSWFFLFFD DPKDHPYMSS SEKDYIISSL
MQQASSGRQS LPIKAMLKSL PLWAIILNSF AFIWSNSLLV TYTPTFISTV LHVNVRENGL
LSSLPYLLAY ICGILAGQMS DFFLTRKIFS IVTVRKLFTT LGSFCPVIFI MCLLYLSYNF
YSTVIFLTLA NSTLSFSYCG QLINALDIAP RYYGFLKAVT ALIGMFGGLI SSTLAGLILN
QDPEYAWHKI FFLMAGINVT CLVFYFLFAK GEIQDWAKEI KTTRL
