ID   NPVF_BOVIN              Reviewed;         196 AA.
AC   Q9GM96;
DT   15-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=Pro-FMRFamide-related neuropeptide VF;
DE   AltName: Full=FMRFamide-related peptides;
DE   Contains:
DE     RecName: Full=Neuropeptide NPSF;
DE   Contains:
DE     RecName: Full=Neuropeptide RFRP-1;
DE   Contains:
DE     RecName: Full=Neuropeptide RFRP-2;
DE   Contains:
DE     RecName: Full=Neuropeptide NPVF;
DE     AltName: Full=Neuropeptide RFRP-3;
DE   Flags: Precursor;
GN   Name=NPVF; Synonyms=RFRP;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913 {ECO:0000312|EMBL:BAB17675.1};
RN   [1] {ECO:0000305}
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Hypothalamus;
RX   PubMed=11025660; DOI=10.1038/35036326;
RA   Hinuma S., Shintani Y., Fukusumi S., Iijima N., Matsumoto Y., Hosoya M.,
RA   Fujii R., Watanabe T., Kikuchi K., Terao Y., Yano T., Yamamoto T.,
RA   Kawamata Y., Habata Y., Asada M., Kitada C., Kurokawa T., Onda H.,
RA   Nishimura O., Tanaka M., Ibata Y., Fujino M.;
RT   "New neuropeptides containing carboxy-terminal RFamide and their receptor
RT   in mammals.";
RL   Nat. Cell Biol. 2:703-708(2000).
RN   [2] {ECO:0000305}
RP   PROTEIN SEQUENCE OF 58-92, AND MASS SPECTROMETRY.
RC   TISSUE=Hypothalamus;
RX   PubMed=11583817; DOI=10.1016/s0167-4889(01)00135-5;
RA   Fukusumi S., Habata Y., Yoshida H., Iijima N., Kawamata Y., Hosoya M.,
RA   Fujii R., Hinuma S., Kitada C., Shintani Y., Suenaga M., Onda H.,
RA   Nishimura O., Tanaka M., Ibata Y., Fujino M.;
RT   "Characteristics and distribution of endogenous RFamide-related peptide-
RT   1.";
RL   Biochim. Biophys. Acta 1540:221-232(2001).
CC   -!- FUNCTION: Neuropeptide RFRP-1 acts as a potent negative regulator of
CC       gonadotropin synthesis and secretion. Neuropeptide NPSF and NPVF
CC       efficiently inhibit forskolin-induced production of cAMP, but RFRP-2
CC       shows no inhibitory activity. Neuropeptide NPVF blocks morphine-induced
CC       analgesia (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- MASS SPECTROMETRY: [Neuropeptide NPSF]: Mass=3996.8; Mass_error=0.6;
CC       Method=Electrospray; Evidence={ECO:0000269|PubMed:11583817};
CC   -!- SIMILARITY: Belongs to the FARP (FMRFamide related peptide) family.
CC       {ECO:0000305}.
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DR   EMBL; AB040291; BAB17675.1; -; mRNA.
DR   RefSeq; NP_776593.1; NM_174168.1.
DR   AlphaFoldDB; Q9GM96; -.
DR   STRING; 9913.ENSBTAP00000025902; -.
DR   PaxDb; Q9GM96; -.
DR   PRIDE; Q9GM96; -.
DR   Ensembl; ENSBTAT00000025902; ENSBTAP00000025902; ENSBTAG00000019447.
DR   GeneID; 281451; -.
DR   KEGG; bta:281451; -.
DR   CTD; 64111; -.
DR   VEuPathDB; HostDB:ENSBTAG00000019447; -.
DR   VGNC; VGNC:32221; NPVF.
DR   eggNOG; ENOG502S5H9; Eukaryota.
DR   GeneTree; ENSGT00390000003271; -.
DR   HOGENOM; CLU_120051_0_0_1; -.
DR   InParanoid; Q9GM96; -.
DR   OMA; NKMPHSA; -.
DR   OrthoDB; 1553087at2759; -.
DR   TreeFam; TF330935; -.
DR   Proteomes; UP000009136; Chromosome 4.
DR   Bgee; ENSBTAG00000019447; Expressed in Ammon's horn and 17 other tissues.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005102; F:signaling receptor binding; IBA:GO_Central.
DR   GO; GO:0032277; P:negative regulation of gonadotropin secretion; IBA:GO_Central.
DR   GO; GO:0007218; P:neuropeptide signaling pathway; IBA:GO_Central.
DR   InterPro; IPR026297; FMRFamide-related/fGRP.
DR   PANTHER; PTHR14403; PTHR14403; 1.
PE   1: Evidence at protein level;
KW   Amidation; Cleavage on pair of basic residues; Direct protein sequencing;
KW   Neuropeptide; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255, ECO:0000303|PubMed:11025660"
FT   PROPEP          22..57
FT                   /evidence="ECO:0000269|PubMed:11583817"
FT                   /id="PRO_0000009913"
FT   PEPTIDE         58..92
FT                   /note="Neuropeptide NPSF"
FT                   /id="PRO_0000009914"
FT   PEPTIDE         81..92
FT                   /note="Neuropeptide RFRP-1"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000401170"
FT   PROPEP          95..99
FT                   /id="PRO_0000009915"
FT   PEPTIDE         101..112
FT                   /note="Neuropeptide RFRP-2"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000009916"
FT   PROPEP          115..121
FT                   /id="PRO_0000009917"
FT   PEPTIDE         123..131
FT                   /note="Neuropeptide NPVF"
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000009918"
FT   PROPEP          134..196
FT                   /id="PRO_0000009919"
FT   MOD_RES         92
FT                   /note="Phenylalanine amide"
FT                   /evidence="ECO:0000250"
FT   MOD_RES         131
FT                   /note="Phenylalanine amide"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   196 AA;  22644 MW;  4F66A2C15AEF3ACD CRC64;
     MEIISLKRFI LLMLATSSLL TSNIFCTDES RMPNLYSKKN YDKYSEPRGD LGWEKERSLT
     FEEVKDWAPK IKMNKPVVNK MPPSAANLPL RFGRNMEEER STRAMAHLPL RLGKNREDSL
     SRWVPNLPQR FGRTTTAKSI TKTLSNLLQQ SMHSPSTNGL LYSMACQPQE IQNPGQKNLR
     RRGFQKIDDA ELKQEK