NPVF_RAT
ID NPVF_RAT Reviewed; 203 AA.
AC Q9ESQ9; Q920A3;
DT 15-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 98.
DE RecName: Full=Pro-FMRFamide-related neuropeptide VF;
DE AltName: Full=FMRFamide-related peptides;
DE Contains:
DE RecName: Full=Neuropeptide NPSF;
DE Contains:
DE RecName: Full=Neuropeptide RFRP-1;
DE Contains:
DE RecName: Full=Neuropeptide NPVF;
DE AltName: Full=Neuropeptide RFRP-2;
DE Flags: Precursor;
GN Name=Npvf; Synonyms=Rfrp;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116 {ECO:0000312|EMBL:BAB17672.1};
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND TISSUE SPECIFICITY.
RC TISSUE=Brain;
RX PubMed=11025660; DOI=10.1038/35036326;
RA Hinuma S., Shintani Y., Fukusumi S., Iijima N., Matsumoto Y., Hosoya M.,
RA Fujii R., Watanabe T., Kikuchi K., Terao Y., Yano T., Yamamoto T.,
RA Kawamata Y., Habata Y., Asada M., Kitada C., Kurokawa T., Onda H.,
RA Nishimura O., Tanaka M., Ibata Y., Fujino M.;
RT "New neuropeptides containing carboxy-terminal RFamide and their receptor
RT in mammals.";
RL Nat. Cell Biol. 2:703-708(2000).
RN [2] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, AND TISSUE SPECIFICITY.
RC STRAIN=Sprague-Dawley; TISSUE=Brain;
RX PubMed=11481330; DOI=10.1074/jbc.m105308200;
RA Liu Q., Guan X.-M., Martin W.J., McDonald T.P., Clements M.K., Jiang Q.,
RA Zeng Z., Jacobson M., Williams D.L. Jr., Yu H., Bomford D., Figueroa D.,
RA Mallee J., Wang R., Evans J., Gould R., Austin C.P.;
RT "Identification and characterization of novel mammalian neuropeptide FF-
RT like peptides that attenuate morphine-induced antinociception.";
RL J. Biol. Chem. 276:36961-36969(2001).
RN [3] {ECO:0000305}
RP PROTEIN SEQUENCE OF 108-125, AMIDATION AT PHE-125, SYNTHESIS, AND MASS
RP SPECTROMETRY.
RC STRAIN=Donryu; TISSUE=Hypothalamus;
RX PubMed=11852091; DOI=10.1016/s0014-5793(02)02275-5;
RA Ukena K., Iwakoshi E., Minakata H., Tsutsui K.;
RT "A novel rat hypothalamic RFamide-related peptide identified by
RT immunoaffinity chromatography and mass spectrometry.";
RL FEBS Lett. 512:255-258(2002).
CC -!- FUNCTION: Neuropeptide RFRP-1 acts as a potent negative regulator of
CC gonadotropin synthesis and secretion. Neuropeptides NPSF and NPVF
CC efficiently inhibit forskolin-induced production of cAMP (By
CC similarity). Neuropeptide NPVF blocks morphine-induced analgesia.
CC {ECO:0000250, ECO:0000269|PubMed:11481330}.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9ESQ9-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9ESQ9-2; Sequence=VSP_039964;
CC -!- TISSUE SPECIFICITY: Isoform 1 is expressed at high levels in the
CC hypothalamus and eye. Isoform 2 is specifically expressed in a region
CC between the dorsomedial hypothalamic and ventromedial hypothalamic
CC nuclei. {ECO:0000269|PubMed:11025660, ECO:0000269|PubMed:11481330}.
CC -!- MASS SPECTROMETRY: [Neuropeptide NPVF]: Mass=1010.54;
CC Method=Electrospray; Evidence={ECO:0000269|PubMed:11852091};
CC -!- SIMILARITY: Belongs to the FARP (FMRFamide related peptide) family.
CC {ECO:0000305}.
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DR EMBL; AB040288; BAB17672.1; -; mRNA.
DR EMBL; AF330059; AAK94203.2; -; mRNA.
DR RefSeq; NP_076442.1; NM_023952.1. [Q9ESQ9-1]
DR AlphaFoldDB; Q9ESQ9; -.
DR STRING; 10116.ENSRNOP00000014437; -.
DR PaxDb; Q9ESQ9; -.
DR PRIDE; Q9ESQ9; -.
DR GeneID; 60570; -.
DR KEGG; rno:60570; -.
DR UCSC; RGD:61815; rat. [Q9ESQ9-1]
DR CTD; 64111; -.
DR RGD; 61815; Npvf.
DR eggNOG; ENOG502S5H9; Eukaryota.
DR InParanoid; Q9ESQ9; -.
DR OrthoDB; 1553087at2759; -.
DR PhylomeDB; Q9ESQ9; -.
DR PRO; PR:Q9ESQ9; -.
DR Proteomes; UP000002494; Unplaced.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0005102; F:signaling receptor binding; IDA:RGD.
DR GO; GO:0032277; P:negative regulation of gonadotropin secretion; ISO:RGD.
DR GO; GO:0007218; P:neuropeptide signaling pathway; IMP:RGD.
DR GO; GO:0019233; P:sensory perception of pain; IMP:RGD.
DR InterPro; IPR026297; FMRFamide-related/fGRP.
DR PANTHER; PTHR14403; PTHR14403; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Amidation; Cleavage on pair of basic residues;
KW Direct protein sequencing; Neuropeptide; Reference proteome; Secreted;
KW Signal.
FT SIGNAL 1..26
FT /evidence="ECO:0000255"
FT PROPEP 27..57
FT /id="PRO_0000009932"
FT PEPTIDE 58..94
FT /note="Neuropeptide NPSF"
FT /id="PRO_0000009933"
FT PEPTIDE 83..94
FT /note="Neuropeptide RFRP-1"
FT /evidence="ECO:0000255"
FT /id="PRO_0000401172"
FT PROPEP 97..106
FT /id="PRO_0000009934"
FT PEPTIDE 108..125
FT /note="Neuropeptide NPVF"
FT /id="PRO_0000009935"
FT PROPEP 128..203
FT /id="PRO_0000009936"
FT REGION 161..186
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 94
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000250"
FT MOD_RES 125
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000269|PubMed:11852091"
FT VAR_SEQ 174..203
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:11481330"
FT /id="VSP_039964"
SQ SEQUENCE 203 AA; 22979 MW; B5F0F26E852BE214 CRC64;
MEIISSKRFI LLTLATSSFL TSNTLCSDEL MMPHFHSKEG YGKYYQLRGI PKGVKERSVT
FQELKDWGAK KDIKMSPAPA NKVPHSAANL PLRFGRNIED RRSPRARANM EAGTMSHFPS
LPQRFGRTTA RRITKTLAGL PQKSLHSLAS SELLYAMTRQ HQEIQSPGQE QPRKRVFTET
DDAERKQEKI GNLQPVLQGA MKL
