NPY4R_HUMAN
ID NPY4R_HUMAN Reviewed; 375 AA.
AC P50391; Q13456; Q5ISU3; Q5T2X9; Q6FH06;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 177.
DE RecName: Full=Neuropeptide Y receptor type 4;
DE Short=NPY4-R;
DE AltName: Full=Pancreatic polypeptide receptor 1;
DE Short=PP1;
GN Name=NPY4R; Synonyms=PPYR1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, AND
RP TISSUE SPECIFICITY.
RC TISSUE=Placenta;
RX PubMed=7592911; DOI=10.1074/jbc.270.45.26762;
RA Bard J.A., Walker M.W., Branchek T.A., Weinshank R.L.;
RT "Cloning and functional expression of a human Y4 subtype receptor for
RT pancreatic polypeptide, neuropeptide Y, and peptide YY.";
RL J. Biol. Chem. 270:26762-26765(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND TISSUE SPECIFICITY.
RX PubMed=7493937; DOI=10.1074/jbc.270.49.29123;
RA Lundell I., Blomqvist A.G., Berglund M.M., Schober D.A., Johnson D.,
RA Statnick M.A., Gadski R.A., Gehlert D.R., Larhammar D.;
RT "Cloning of a human receptor of the NPY receptor family with high affinity
RT for pancreatic polypeptide and peptide YY.";
RL J. Biol. Chem. 270:29123-29128(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA], AND VARIANT SER-99.
RX PubMed=8643460; DOI=10.1073/pnas.93.10.4661;
RA Yan H., Yang J., Marasco J., Yamaguchi K., Brenner S., Collins F.,
RA Karbon W.;
RT "Cloning and functional expression of cDNAs encoding human and rat
RT pancreatic polypeptide receptors.";
RL Proc. Natl. Acad. Sci. U.S.A. 93:4661-4665(1996).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA], AND VARIANT SER-99.
RA Reichwald K., Petz U., Roschmann E., Platzer M.;
RT "PPYR1: identification of new untranslated 5' exon by in silico and
RT experimental evaluation of gene structure.";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Kopatz S.A., Aronstam R.S., Sharma S.V.;
RT "cDNA clones of human proteins involved in signal transduction sequenced by
RT the Guthrie cDNA resource center (www.cdna.org).";
RL Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT CYS-240.
RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15164054; DOI=10.1038/nature02462;
RA Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L.,
RA Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K.,
RA Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L.,
RA Taylor A., Battles J., Bird C.P., Ainscough R., Almeida J.P.,
RA Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J.,
RA Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y.,
RA Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P.,
RA Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N.,
RA Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A.,
RA Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C.,
RA Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D.,
RA Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C.,
RA Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K.,
RA Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A.,
RA Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S.,
RA McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S.,
RA Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V.,
RA Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A.,
RA Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M.,
RA Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A.,
RA Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P.,
RA Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y.,
RA Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D.,
RA Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.;
RT "The DNA sequence and comparative analysis of human chromosome 10.";
RL Nature 429:375-381(2004).
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT SER-99.
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Receptor for neuropeptide Y and peptide YY. The rank order of
CC affinity of this receptor for pancreatic polypeptides is PP, PP (2-36)
CC and [Ile-31, Gln-34] PP > [Pro-34] PYY > PYY and [Leu-31, Pro-34] NPY >
CC NPY > PYY (3-36) and NPY (2-36) > PP (13-36) > PP (31-36) > NPY free
CC acid. {ECO:0000269|PubMed:7592911}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305|PubMed:7592911};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Highest levels found in brain, coronary artery and
CC ileum. Low levels in pancreas and kidney. Detected in colon and small
CC intestine. {ECO:0000269|PubMed:7493937, ECO:0000269|PubMed:7592911}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR EMBL; U35232; AAC50280.1; -; Genomic_DNA.
DR EMBL; Z66526; CAA91433.1; -; Genomic_DNA.
DR EMBL; U42387; AAB07759.1; -; mRNA.
DR EMBL; AY649985; AAV68196.1; -; mRNA.
DR EMBL; AY649986; AAV68197.1; -; mRNA.
DR EMBL; AY268432; AAP23199.1; -; Genomic_DNA.
DR EMBL; CR541950; CAG46748.1; -; mRNA.
DR EMBL; AL390716; CAI13318.1; -; Genomic_DNA.
DR EMBL; BC096238; AAH96238.1; -; mRNA.
DR CCDS; CCDS81459.1; -.
DR PIR; G02300; G02300.
DR PIR; I39182; I39182.
DR RefSeq; NP_001265723.1; NM_001278794.1.
DR RefSeq; NP_001265724.1; NM_001278795.1.
DR RefSeq; NP_005963.4; NM_005972.5.
DR RefSeq; XP_005277701.1; XM_005277644.3.
DR RefSeq; XP_006717608.1; XM_006717545.3.
DR RefSeq; XP_011537416.1; XM_011539114.2.
DR RefSeq; XP_011537417.1; XM_011539115.2.
DR RefSeq; XP_011538238.1; XM_011539936.2.
DR RefSeq; XP_011538239.1; XM_011539937.2.
DR RefSeq; XP_011547675.1; XM_011549373.2.
DR RefSeq; XP_011547676.1; XM_011549374.2.
DR RefSeq; XP_011547677.1; XM_011549375.2.
DR RefSeq; XP_011547678.1; XM_011549376.2.
DR RefSeq; XP_016854962.1; XM_016999473.1.
DR RefSeq; XP_016854963.1; XM_016999474.1.
DR RefSeq; XP_016870952.1; XM_017015463.1.
DR RefSeq; XP_016870953.1; XM_017015464.1.
DR RefSeq; XP_016871875.1; XM_017016386.1.
DR RefSeq; XP_016871876.1; XM_017016387.1.
DR AlphaFoldDB; P50391; -.
DR BMRB; P50391; -.
DR SMR; P50391; -.
DR BioGRID; 111532; 9.
DR BindingDB; P50391; -.
DR ChEMBL; CHEMBL4877; -.
DR DrugBank; DB05045; Obinepitide.
DR DrugBank; DB05004; Peptide YY (3-36).
DR DrugBank; DB05085; TM30339.
DR GuidetoPHARMACOLOGY; 307; -.
DR GlyGen; P50391; 4 sites.
DR iPTMnet; P50391; -.
DR PhosphoSitePlus; P50391; -.
DR BioMuta; NPY4R; -.
DR DMDM; 1709423; -.
DR PaxDb; P50391; -.
DR PRIDE; P50391; -.
DR ProteomicsDB; 56215; -.
DR Antibodypedia; 13604; 248 antibodies from 27 providers.
DR DNASU; 5540; -.
DR Ensembl; ENST00000374312.5; ENSP00000363431.1; ENSG00000204174.8.
DR Ensembl; ENST00000612632.3; ENSP00000480883.1; ENSG00000204174.8.
DR GeneID; 100996758; -.
DR GeneID; 5540; -.
DR KEGG; hsa:100996758; -.
DR KEGG; hsa:5540; -.
DR MANE-Select; ENST00000576178.7; ENSP00000490174.1; NM_001395253.1; NP_001382182.1.
DR UCSC; uc031wfc.1; human.
DR CTD; 100996758; -.
DR CTD; 5540; -.
DR DisGeNET; 100996758; -.
DR DisGeNET; 5540; -.
DR GeneCards; NPY4R; -.
DR HGNC; HGNC:9329; NPY4R.
DR HPA; ENSG00000204174; Tissue enhanced (intestine, skin).
DR MIM; 601790; gene.
DR neXtProt; NX_P50391; -.
DR PharmGKB; PA33692; -.
DR VEuPathDB; HostDB:ENSG00000204174; -.
DR eggNOG; KOG3656; Eukaryota.
DR GeneTree; ENSGT00940000161927; -.
DR HOGENOM; CLU_009579_6_1_1; -.
DR InParanoid; P50391; -.
DR OrthoDB; 609835at2759; -.
DR PhylomeDB; P50391; -.
DR TreeFam; TF315303; -.
DR PathwayCommons; P50391; -.
DR Reactome; R-HSA-375276; Peptide ligand-binding receptors.
DR Reactome; R-HSA-418594; G alpha (i) signalling events.
DR SignaLink; P50391; -.
DR SIGNOR; P50391; -.
DR BioGRID-ORCS; 100996758; 2 hits in 78 CRISPR screens.
DR BioGRID-ORCS; 5540; 45 hits in 1051 CRISPR screens.
DR GeneWiki; Pancreatic_polypeptide_receptor_1; -.
DR Pharos; P50391; Tchem.
DR PRO; PR:P50391; -.
DR Proteomes; UP000005640; Chromosome 10.
DR RNAct; P50391; protein.
DR Bgee; ENSG00000204174; Expressed in rectum and 40 other tissues.
DR Genevisible; P50391; HS.
DR GO; GO:0005887; C:integral component of plasma membrane; TAS:ProtInc.
DR GO; GO:0016020; C:membrane; IDA:GO_Central.
DR GO; GO:0043005; C:neuron projection; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0004930; F:G protein-coupled receptor activity; TAS:ProtInc.
DR GO; GO:0042923; F:neuropeptide binding; IBA:GO_Central.
DR GO; GO:0008188; F:neuropeptide receptor activity; IBA:GO_Central.
DR GO; GO:0001602; F:pancreatic polypeptide receptor activity; IDA:GO_Central.
DR GO; GO:0017046; F:peptide hormone binding; IPI:GO_Central.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IDA:GO_Central.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR001933; NPY4_rcpt.
DR InterPro; IPR000611; NPY_rcpt.
DR PANTHER; PTHR24235:SF25; PTHR24235:SF25; 1.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PRINTS; PR01015; NRPEPTIDEY4R.
DR PRINTS; PR01012; NRPEPTIDEYR.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Disulfide bond; G-protein coupled receptor; Glycoprotein;
KW Lipoprotein; Membrane; Palmitate; Receptor; Reference proteome; Transducer;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..375
FT /note="Neuropeptide Y receptor type 4"
FT /id="PRO_0000069935"
FT TOPO_DOM 1..39
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 40..60
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 61..87
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 88..108
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 109..116
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 117..137
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 138..155
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 156..176
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 177..212
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 213..233
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 234..263
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 264..284
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 285..301
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 302..322
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 323..375
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT LIPID 340
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000255"
FT CARBOHYD 2
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 19
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 29
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 187
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 114..201
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
FT VARIANT 99
FT /note="A -> S (in dbSNP:rs2229967)"
FT /evidence="ECO:0000269|PubMed:15489334,
FT ECO:0000269|PubMed:8643460, ECO:0000269|Ref.4"
FT /id="VAR_030337"
FT VARIANT 240
FT /note="R -> C (in dbSNP:rs3824733)"
FT /evidence="ECO:0000269|Ref.6"
FT /id="VAR_030338"
SQ SEQUENCE 375 AA; 42195 MW; 0081DBA51436EE22 CRC64;
MNTSHLLALL LPKSPQGENR SKPLGTPYNF SEHCQDSVDV MVFIVTSYSI ETVVGVLGNL
CLMCVTVRQK EKANVTNLLI ANLAFSDFLM CLLCQPLTAV YTIMDYWIFG ETLCKMSAFI
QCMSVTVSIL SLVLVALERH QLIINPTGWK PSISQAYLGI VLIWVIACVL SLPFLANSIL
ENVFHKNHSK ALEFLADKVV CTESWPLAHH RTIYTTFLLL FQYCLPLGFI LVCYARIYRR
LQRQGRVFHK GTYSLRAGHM KQVNVVLVVM VVAFAVLWLP LHVFNSLEDW HHEAIPICHG
NLIFLVCHLL AMASTCVNPF IYGFLNTNFK KEIKALVLTC QQSAPLEESE HLPLSTVHTE
VSKGSLRLSG RSNPI
