NPY4_ARATH
ID NPY4_ARATH Reviewed; 481 AA.
AC O64814;
DT 28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 25-MAY-2022, entry version 129.
DE RecName: Full=BTB/POZ domain-containing protein NPY4;
DE AltName: Full=Protein NAKED PINS IN YUC MUTANTS 4;
GN Name=NPY4; OrderedLocusNames=At2g23050; ORFNames=F21P24.11;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=12481096; DOI=10.1104/pp.010207;
RA Xiao Y.-L., Malik M., Whitelaw C.A., Town C.D.;
RT "Cloning and sequencing of cDNAs for hypothetical genes from chromosome 2
RT of Arabidopsis.";
RL Plant Physiol. 130:2118-2128(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=16244158; DOI=10.1104/pp.105.063479;
RA Xiao Y.-L., Smith S.R., Ishmael N., Redman J.C., Kumar N., Monaghan E.L.,
RA Ayele M., Haas B.J., Wu H.C., Town C.D.;
RT "Analysis of the cDNAs of hypothetical genes on Arabidopsis chromosome 2
RT reveals numerous transcript variants.";
RL Plant Physiol. 139:1323-1337(2005).
RN [7]
RP DOMAIN BTB.
RX PubMed=15749712; DOI=10.1074/jbc.m413247200;
RA Gingerich D.J., Gagne J.M., Salter D.W., Hellmann H., Estelle M., Ma L.,
RA Vierstra R.D.;
RT "Cullins 3a and 3b assemble with members of the broad
RT complex/tramtrack/bric-a-brac (BTB) protein family to form essential
RT ubiquitin-protein ligases (E3s) in Arabidopsis.";
RL J. Biol. Chem. 280:18810-18821(2005).
RN [8]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=19075219; DOI=10.1073/pnas.0809761106;
RA Cheng Y., Qin G., Dai X., Zhao Y.;
RT "NPY genes and AGC kinases define two key steps in auxin-mediated
RT organogenesis in Arabidopsis.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:21017-21022(2008).
RN [9]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=20833732; DOI=10.1093/mp/ssq052;
RA Li Y., Dai X., Cheng Y., Zhao Y.;
RT "NPY genes play an essential role in root gravitropic responses in
RT Arabidopsis.";
RL Mol. Plant 4:171-179(2011).
CC -!- FUNCTION: May act as a substrate-specific adapter of an E3 ubiquitin-
CC protein ligase complex (CUL3-RBX1-BTB) which mediates the
CC ubiquitination and subsequent proteasomal degradation of target
CC proteins (By similarity). May play an essential role in auxin-mediated
CC organogenesis and in root gravitropic responses. {ECO:0000250,
CC ECO:0000269|PubMed:19075219, ECO:0000269|PubMed:20833732}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- TISSUE SPECIFICITY: Expressed in the hypocotyl cells that would
CC differentiate into vascular bundles. Highly expressed in primary root
CC tips. {ECO:0000269|PubMed:19075219, ECO:0000269|PubMed:20833732}.
CC -!- DOMAIN: The BTB/POZ domain mediates the interaction with some component
CC of ubiquitin ligase complexes. {ECO:0000269|PubMed:15749712}.
CC -!- SIMILARITY: Belongs to the NPH3 family. {ECO:0000255|PROSITE-
CC ProRule:PRU00982}.
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DR EMBL; AC004401; AAC17821.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07400.1; -; Genomic_DNA.
DR EMBL; AY102541; AAM76746.1; -; mRNA.
DR EMBL; AK117486; BAC42149.1; -; mRNA.
DR EMBL; BT005613; AAO64033.1; -; mRNA.
DR EMBL; AY954795; AAX55121.1; -; mRNA.
DR PIR; H84619; H84619.
DR RefSeq; NP_179887.1; NM_127869.3.
DR AlphaFoldDB; O64814; -.
DR BioGRID; 2188; 1.
DR IntAct; O64814; 1.
DR STRING; 3702.AT2G23050.1; -.
DR PaxDb; O64814; -.
DR PRIDE; O64814; -.
DR ProteomicsDB; 251073; -.
DR EnsemblPlants; AT2G23050.1; AT2G23050.1; AT2G23050.
DR GeneID; 816835; -.
DR Gramene; AT2G23050.1; AT2G23050.1; AT2G23050.
DR KEGG; ath:AT2G23050; -.
DR Araport; AT2G23050; -.
DR TAIR; locus:2045314; AT2G23050.
DR eggNOG; ENOG502QSYM; Eukaryota.
DR HOGENOM; CLU_005994_5_2_1; -.
DR InParanoid; O64814; -.
DR OMA; QKFIAIT; -.
DR OrthoDB; 516861at2759; -.
DR PhylomeDB; O64814; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:O64814; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O64814; baseline and differential.
DR Genevisible; O64814; AT.
DR GO; GO:0071944; C:cell periphery; IDA:TAIR.
DR GO; GO:0009958; P:positive gravitropism; IGI:TAIR.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.30.710.10; -; 1.
DR InterPro; IPR000210; BTB/POZ_dom.
DR InterPro; IPR043454; NPH3/RPT2-like.
DR InterPro; IPR027356; NPH3_dom.
DR InterPro; IPR011333; SKP1/BTB/POZ_sf.
DR PANTHER; PTHR32370; PTHR32370; 1.
DR Pfam; PF00651; BTB; 1.
DR Pfam; PF03000; NPH3; 1.
DR SUPFAM; SSF54695; SSF54695; 1.
DR PROSITE; PS50097; BTB; 1.
DR PROSITE; PS51649; NPH3; 1.
PE 2: Evidence at transcript level;
KW Phosphoprotein; Reference proteome; Ubl conjugation pathway.
FT CHAIN 1..481
FT /note="BTB/POZ domain-containing protein NPY4"
FT /id="PRO_0000409566"
FT DOMAIN 29..102
FT /note="BTB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00037"
FT DOMAIN 207..450
FT /note="NPH3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00982"
FT REGION 456..481
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 391
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:Q9FMF5"
SQ SEQUENCE 481 AA; 54954 MW; 7D10C11E898F43E8 CRC64;
MKFMKLGTKP DSFLSKGDNV RYVTNELETE IIIIIGNVKF YLHKFPLLSK SGFLQKHIAT
SKNEEEKKNQ IDEIDISEIP GGSVAFEICV KFCYGITVTL NAYNVVAVRC AAEFLEMNET
FEKSNLVYKI DVFLNSTIFR SWKDSIIVLQ TTKDLLSDDS EELVKRCLGS IASTASIDTS
KVKWSYTYNR KKKLEKVRKP EDGVPKDWWV EDLCELHIDL YKQAIKAIKN RGKVPSNVIG
EALHAYAIRR IAGFSKESMQ LIDRSLINTI IELLPDEKGN ISSSFLTKLH RASIFLGCEE
TVKEKLKKRV SEQLEETTVN DILMYDLDMV QSLVKEFMNR DPKTHSKVSV AKLIDGYLAE
KSRDPNLPLQ NFLSLAETLS SFPRHSHDVL YRAIDMFLKE HSGISKSEKK RVCGLMDCRK
LSAEACEHAV QNERLPMRVI VQVLFFEQIR ANGSSTGYST PELTTTTLNT EDDEWDHEKE
F
