NPY5_ARATH
ID NPY5_ARATH Reviewed; 580 AA.
AC Q0WL52; F4JS42; Q940F9; Q9SZF2;
DT 28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 2.
DT 25-MAY-2022, entry version 87.
DE RecName: Full=BTB/POZ domain-containing protein NPY5;
DE AltName: Full=Protein NAKED PINS IN YUC MUTANTS 5;
GN Name=NPY5; OrderedLocusNames=At4g37590; ORFNames=F19F18.80;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP DOMAIN BTB.
RX PubMed=15749712; DOI=10.1074/jbc.m413247200;
RA Gingerich D.J., Gagne J.M., Salter D.W., Hellmann H., Estelle M., Ma L.,
RA Vierstra R.D.;
RT "Cullins 3a and 3b assemble with members of the broad
RT complex/tramtrack/bric-a-brac (BTB) protein family to form essential
RT ubiquitin-protein ligases (E3s) in Arabidopsis.";
RL J. Biol. Chem. 280:18810-18821(2005).
RN [6]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=19075219; DOI=10.1073/pnas.0809761106;
RA Cheng Y., Qin G., Dai X., Zhao Y.;
RT "NPY genes and AGC kinases define two key steps in auxin-mediated
RT organogenesis in Arabidopsis.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:21017-21022(2008).
RN [7]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=20833732; DOI=10.1093/mp/ssq052;
RA Li Y., Dai X., Cheng Y., Zhao Y.;
RT "NPY genes play an essential role in root gravitropic responses in
RT Arabidopsis.";
RL Mol. Plant 4:171-179(2011).
CC -!- FUNCTION: May act as a substrate-specific adapter of an E3 ubiquitin-
CC protein ligase complex (CUL3-RBX1-BTB) which mediates the
CC ubiquitination and subsequent proteasomal degradation of target
CC proteins (By similarity). May play an essential role in auxin-mediated
CC organogenesis and in root gravitropic responses. {ECO:0000250,
CC ECO:0000269|PubMed:19075219, ECO:0000269|PubMed:20833732}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- TISSUE SPECIFICITY: Expressed in the cotyledon cells that would
CC differentiate into vascular bundles. Also detected in the apical
CC meristem and young flowers. Highly expressed in primary root tips.
CC {ECO:0000269|PubMed:19075219, ECO:0000269|PubMed:20833732}.
CC -!- DOMAIN: The BTB/POZ domain mediates the interaction with some component
CC of ubiquitin ligase complexes. {ECO:0000269|PubMed:15749712}.
CC -!- SIMILARITY: Belongs to the NPH3 family. {ECO:0000255|PROSITE-
CC ProRule:PRU00982}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB38298.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB80424.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL035605; CAB38298.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161591; CAB80424.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE86814.1; -; Genomic_DNA.
DR EMBL; AY054668; AAK96859.1; -; mRNA.
DR EMBL; AY081497; AAM10059.1; -; mRNA.
DR EMBL; AK230356; BAF02155.1; -; mRNA.
DR PIR; T04716; T04716.
DR RefSeq; NP_568030.1; NM_119923.4.
DR AlphaFoldDB; Q0WL52; -.
DR STRING; 3702.AT4G37590.1; -.
DR iPTMnet; Q0WL52; -.
DR PaxDb; Q0WL52; -.
DR PRIDE; Q0WL52; -.
DR ProteomicsDB; 249049; -.
DR EnsemblPlants; AT4G37590.1; AT4G37590.1; AT4G37590.
DR GeneID; 829914; -.
DR Gramene; AT4G37590.1; AT4G37590.1; AT4G37590.
DR KEGG; ath:AT4G37590; -.
DR Araport; AT4G37590; -.
DR TAIR; locus:2120125; AT4G37590.
DR eggNOG; ENOG502QSYM; Eukaryota.
DR HOGENOM; CLU_005994_5_2_1; -.
DR InParanoid; Q0WL52; -.
DR OMA; ITDYAKY; -.
DR OrthoDB; 496456at2759; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q0WL52; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q0WL52; baseline and differential.
DR Genevisible; Q0WL52; AT.
DR GO; GO:0071944; C:cell periphery; IDA:TAIR.
DR GO; GO:0009908; P:flower development; IGI:TAIR.
DR GO; GO:0009958; P:positive gravitropism; IGI:TAIR.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.30.710.10; -; 1.
DR InterPro; IPR000210; BTB/POZ_dom.
DR InterPro; IPR043454; NPH3/RPT2-like.
DR InterPro; IPR027356; NPH3_dom.
DR InterPro; IPR011333; SKP1/BTB/POZ_sf.
DR PANTHER; PTHR32370; PTHR32370; 1.
DR Pfam; PF00651; BTB; 1.
DR Pfam; PF03000; NPH3; 1.
DR SUPFAM; SSF54695; SSF54695; 1.
DR PROSITE; PS50097; BTB; 1.
DR PROSITE; PS51649; NPH3; 1.
PE 2: Evidence at transcript level;
KW Phosphoprotein; Reference proteome; Ubl conjugation pathway.
FT CHAIN 1..580
FT /note="BTB/POZ domain-containing protein NPY5"
FT /id="PRO_0000409567"
FT DOMAIN 29..108
FT /note="BTB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00037"
FT DOMAIN 214..465
FT /note="NPH3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00982"
FT REGION 468..497
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 548..580
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 468..494
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 551..572
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 406
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:Q9FMF5"
FT CONFLICT 26
FT /note="E -> D (in Ref. 3; AAK96859/AAM10059)"
FT /evidence="ECO:0000305"
FT CONFLICT 555
FT /note="I -> L (in Ref. 4; BAF02155)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 580 AA; 64571 MW; 5C95BEB33C33AC78 CRC64;
MKFMKLGSKP DSFQSEGDNV RYVSSELATD VIVIIGDVKF YLHKFPLLSK SARLQKLITT
STSSSNEENQ IHHHHHEDEI EIAEIPGGPA SFEICAKFCY GMTVTLNAYN VVAARCAAEF
LEMYETVEKG NLVYKIEVFL NSSILQSWKD SIIVLQTTRA LSPYSEELKL TGRCLDSIAS
RASIDTSKVE WSYTYSKKKN LDNGLRKPQA VPRDWWVEDL CDLHIDLYKR ALATIEARGN
VSADVIGEAL HAYAIKRIPG FSKSSSVQVT DFAKYRALAD SIIELIPDEK RSVSSSFLTK
LLRASIFLGC DEVAGLKNRV GERLDEANLG DVLLYDVELM QSLVEVFLKS RDPREDDVTA
KASVAKLVDG YLAEKSRDSD NLPLQKFLSL AEMVSSFPRQ SHDGVYRAID MFLKEHPEMN
KSEKKRICRL MDCRKLSAEA CAHAVQNERL PMRVVVQVLF FEQVRANNNG SSSTGNSTPE
VIPASRSTNT TDQEDTECWD TEDIKALRGE LANLRLAKNQ QESHNQGKLM KGGGLGVSRV
FSKLWSGKER SGDMISSSGT SSPGSVNDDS KSSSSTSKKH
