ID   NP_ADEM1                Reviewed;         199 AA.
AC   O10440;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   03-AUG-2022, entry version 70.
DE   RecName: Full=Pre-histone-like nucleoprotein {ECO:0000255|HAMAP-Rule:MF_04056};
DE   AltName: Full=Pre-core protein VII {ECO:0000255|HAMAP-Rule:MF_04056};
DE            Short=pVII {ECO:0000255|HAMAP-Rule:MF_04056};
DE   Contains:
DE     RecName: Full=Histone-like nucleoprotein {ECO:0000255|HAMAP-Rule:MF_04056};
DE              Short=NP {ECO:0000255|HAMAP-Rule:MF_04056};
DE     AltName: Full=Core protein VII {ECO:0000255|HAMAP-Rule:MF_04056};
GN   Name=L2 {ECO:0000255|HAMAP-Rule:MF_04056};
OS   Murine adenovirus A serotype 1 (MAdV-1) (Murine adenovirus 1).
OC   Viruses; Varidnaviria; Bamfordvirae; Preplasmiviricota; Tectiliviricetes;
OC   Rowavirales; Adenoviridae; Mastadenovirus; Murine mastadenovirus A.
OX   NCBI_TaxID=10530;
OH   NCBI_TaxID=10090; Mus musculus (Mouse).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Meissner J.D., Hirsch G.N., Larue E.A., Fulcher R.A., Spindler K.R.;
RL   Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Plays a role in the inhibition of host immune response within
CC       the nucleus. Interacts with cellular nucleosomes and immobilizes the
CC       host immune danger signal HMGB1 on chromatin. In turn, prevents HMGB1
CC       release out of the cell and thus decreases inflammation. Also plays a
CC       role in the wrapping and condensation of the viral DNA. May also
CC       promote viral genome import into the nucleus. {ECO:0000255|HAMAP-
CC       Rule:MF_04056}.
CC   -!- SUBUNIT: Interacts with the core-capsid bridging protein; this
CC       interaction bridges the virus core to the capsid. Interacts with host
CC       NPM1; this interaction might play a role in placing the pre-histone-
CC       like nucleoprotein on the viral DNA or regulating viral gene
CC       expression. Interacts with host HMGB1; this interaction inhibits host
CC       immune response. {ECO:0000255|HAMAP-Rule:MF_04056}.
CC   -!- SUBCELLULAR LOCATION: [Histone-like nucleoprotein]: Virion
CC       {ECO:0000255|HAMAP-Rule:MF_04056}. Note=Located inside the capsid in
CC       association with the viral DNA (core). Present in about 1070 copies per
CC       virion. {ECO:0000255|HAMAP-Rule:MF_04056}.
CC   -!- SUBCELLULAR LOCATION: [Pre-histone-like nucleoprotein]: Host nucleus,
CC       host nucleolus {ECO:0000255|HAMAP-Rule:MF_04056}.
CC   -!- INDUCTION: Expressed in the late phase of the viral replicative cycle.
CC       {ECO:0000255|HAMAP-Rule:MF_04056}.
CC   -!- PTM: Cleaved near the N-terminus by the viral protease during virion
CC       maturation to form the mature protein. {ECO:0000255|HAMAP-
CC       Rule:MF_04056}.
CC   -!- MISCELLANEOUS: All late proteins expressed from the major late promoter
CC       are produced by alternative splicing and alternative polyadenylation of
CC       the same gene giving rise to non-overlapping ORFs. A leader sequence is
CC       present in the N-terminus of all these mRNAs and is recognized by the
CC       viral shutoff protein to provide expression although conventional
CC       translation via ribosome scanning from the cap has been shut off in the
CC       host cell. {ECO:0000255|HAMAP-Rule:MF_04056}.
CC   -!- SIMILARITY: Belongs to the adenoviridae histone-like nucleoprotein
CC       family. {ECO:0000255|HAMAP-Rule:MF_04056, ECO:0000305}.
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DR   EMBL; U95843; AAB53755.1; -; Genomic_DNA.
DR   PRIDE; O10440; -.
DR   GO; GO:0044196; C:host cell nucleolus; IEA:UniProtKB-SubCell.
DR   GO; GO:0019028; C:viral capsid; IEA:InterPro.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-UniRule.
DR   GO; GO:0075732; P:viral penetration into host nucleus; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_04056; ADV_PVII; 1.
DR   InterPro; IPR004912; Adeno_VII.
DR   Pfam; PF03228; Adeno_VII; 1.
PE   3: Inferred from homology;
KW   Acetylation; DNA-binding; Host nucleus; Host-virus interaction;
KW   Late protein; Phosphoprotein; Viral penetration into host nucleus; Virion;
KW   Virus entry into host cell.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04056"
FT   CHAIN           2..199
FT                   /note="Pre-histone-like nucleoprotein"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04056"
FT                   /id="PRO_0000421442"
FT   PROPEP          2..23
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04056"
FT                   /id="PRO_0000036595"
FT   CHAIN           24..199
FT                   /note="Histone-like nucleoprotein"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04056"
FT                   /id="PRO_0000036596"
FT   MOTIF           189..199
FT                   /note="Nuclear localization signal"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04056"
FT   SITE            23..24
FT                   /note="Cleavage; by viral protease"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04056"
FT   MOD_RES         2
FT                   /note="N-acetylserine; by host"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04056"
FT   MOD_RES         26
FT                   /note="N6-acetyllysine; by host"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04056"
SQ   SEQUENCE   199 AA;  21927 MW;  99588540A055C39B CRC64;
     MSILISPSDN TGWGLGTGKM YGGARKRSAE HPVHVRSYWR AAWGSRNRRR VATVAAEDAE
     APQLEDVAQA PATVPIVRRH RRRVGGSART RGLRKSARVR AAARAIVRAV NGAAAAAAPT
     VPASANFAAM VGAIANARAA YRHRRSRAGI NPVPASRSTT RVRLPRTVRF HPSMGAFHRR
     WWRIHTRGRR KASVRRRRT