NQO12_PARDE
ID NQO12_PARDE Reviewed; 703 AA.
AC P29924;
DT 01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1993, sequence version 1.
DT 25-MAY-2022, entry version 85.
DE RecName: Full=NADH-quinone oxidoreductase chain 12;
DE EC=7.1.1.-;
DE AltName: Full=NADH dehydrogenase I, chain 12;
DE AltName: Full=NDH-1, chain 12;
GN Name=nqo12 {ECO:0000303|PubMed:8422400};
OS Paracoccus denitrificans.
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC Rhodobacteraceae; Paracoccus.
OX NCBI_TaxID=266;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 13543 / NRRL B-3784 / NRC 449;
RX PubMed=8422400; DOI=10.1021/bi00054a030;
RA Xu X., Matsuno-Yagi A., Yagi T.;
RT "DNA sequencing of the seven remaining structural genes of the gene cluster
RT encoding the energy-transducing NADH-quinone oxidoreductase of Paracoccus
RT denitrificans.";
RL Biochemistry 32:968-981(1993).
CC -!- FUNCTION: NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur
CC (Fe-S) centers, to quinones in the respiratory chain. The immediate
CC electron acceptor for the enzyme in this species is believed to be
CC ubiquinone. Couples the redox reaction to proton translocation (for
CC every two electrons transferred, four hydrogen ions are translocated
CC across the cytoplasmic membrane), and thus conserves the redox energy
CC in a proton gradient.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) +
CC NAD(+); Xref=Rhea:RHEA:57888, ChEBI:CHEBI:15378, ChEBI:CHEBI:24646,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:132124;
CC -!- SUBUNIT: NDH-1 is composed of at least 14 different subunits, Nqo1 to
CC Nqo14. The complex has a L-shaped structure, with the hydrophobic arm
CC (subunits Nqo7, Nqo8, Nqo10 to Nqo14) embedded in the inner membrane
CC and the hydrophilic peripheral arm (subunits Nqo1 to Nqo6, Nqo9)
CC protruding into the bacterial cytoplasm. The hydrophilic domain
CC contains all the redox centers.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the complex I subunit 5 family. {ECO:0000305}.
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DR EMBL; L02354; AAA25598.1; -; Genomic_DNA.
DR PIR; H45456; H45456.
DR AlphaFoldDB; P29924; -.
DR SMR; P29924; -.
DR TCDB; 3.D.1.2.1; the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:InterPro.
DR GO; GO:0048038; F:quinone binding; IEA:UniProtKB-KW.
DR GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
DR InterPro; IPR018393; NADHpl_OxRdtase_5_subgr.
DR InterPro; IPR001750; ND/Mrp_mem.
DR InterPro; IPR003945; NU5C-like.
DR InterPro; IPR001516; Proton_antipo_N.
DR PANTHER; PTHR42829; PTHR42829; 1.
DR Pfam; PF00361; Proton_antipo_M; 1.
DR Pfam; PF00662; Proton_antipo_N; 1.
DR TIGRFAMs; TIGR01974; NDH_I_L; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Membrane; NAD; Quinone; Translocase;
KW Transmembrane; Transmembrane helix; Ubiquinone.
FT CHAIN 1..703
FT /note="NADH-quinone oxidoreductase chain 12"
FT /id="PRO_0000118211"
FT TRANSMEM 4..24
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 30..50
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 79..99
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 116..136
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 138..158
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 179..199
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 224..244
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 256..276
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 290..310
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 325..345
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 346..366
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 381..401
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 415..435
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 475..495
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 580..600
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 679..699
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 703 AA; 77706 MW; 433993D4081B14CA CRC64;
MEKFVLFAPL IASLIAGLGW RAIGEKAAQY LTTGVLFLSC LISWYLFLSF DGVPRHIPVL
DWVVTGDFHA EWAIRLDRLT AIMLIVVTTV SALVHMYSLG YMAHDDNWTH DEHYKARFFA
YLSFFTFAML MLVTADNLLQ MFFGWEGVGV ASYLLIGFYY KKASANAAAM KAFIVNRVGD
FGFLLGIFGI YWLTGSVQFD EIFRQVPQLA QTEIDFLWRD WNAANLLGFL LFVGAMGKSA
QLLLHTWLPD AMEGPTPVSA LIHAATMVTA GVFLVCRMSP LYEFAPDAKN FIVIIGATTA
FFAATVGLVQ NDIKRVIAYS TCSQLGYMFV AAGVGVYSAA MFHLLTHAFF KAMLFLGAGS
VIHAMHHEQD MRNYGGLRKK IPLTFWAMMI GTFAITGVGI PLTHLGFAGF LSKDAIIESA
YAGSGYAFWL LVIAACFTSF YSWRLIFLTF YGKPRGDHHA HDHAHESPPV MTIPLGVLAI
GAVFAGMVWY GPFFGDHHKV TEYFHIAGAH HEAAEGEEAE HATAEAPVEH AVADTATAEG
EAAAEAEHAE IAAPVGGAIY MHPDNHIMDE AHHAPAWVKV SPFVAMVLGL ITAWTFYIAN
PSLPRRLAAH EPALYRFLLN KWYFDEIYEF IFVRPAKWLG RVLWKGGDGA VIDGTINGVA
MGLIPRLTRA AVRVQSGYLF HYAFAMVLGI VGLLIWVMMR GAH
