AROF_SALTY
ID AROF_SALTY Reviewed; 356 AA.
AC P0A1B5; P21307;
DT 01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2005, sequence version 1.
DT 03-AUG-2022, entry version 90.
DE RecName: Full=Phospho-2-dehydro-3-deoxyheptonate aldolase, Tyr-sensitive;
DE EC=2.5.1.54;
DE AltName: Full=3-deoxy-D-arabino-heptulosonate 7-phosphate synthase;
DE AltName: Full=DAHP synthase;
DE AltName: Full=Phospho-2-keto-3-deoxyheptonate aldolase;
GN Name=aroF; OrderedLocusNames=STM2670;
OS Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=99287;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1970560; DOI=10.1128/jb.172.5.2259-2266.1990;
RA Muday G.K., Herrmann K.M.;
RT "Regulation of the Salmonella typhimurium aroF gene in Escherichia coli.";
RL J. Bacteriol. 172:2259-2266(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=11677609; DOI=10.1038/35101614;
RA McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA Wilson R.K.;
RT "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL Nature 413:852-856(2001).
CC -!- FUNCTION: Stereospecific condensation of phosphoenolpyruvate (PEP) and
CC D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D-arabino-
CC heptulosonate-7-phosphate (DAHP).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=D-erythrose 4-phosphate + H2O + phosphoenolpyruvate = 7-
CC phospho-2-dehydro-3-deoxy-D-arabino-heptonate + phosphate;
CC Xref=Rhea:RHEA:14717, ChEBI:CHEBI:15377, ChEBI:CHEBI:16897,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:58394, ChEBI:CHEBI:58702; EC=2.5.1.54;
CC -!- PATHWAY: Metabolic intermediate biosynthesis; chorismate biosynthesis;
CC chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step
CC 1/7.
CC -!- MISCELLANEOUS: There are 3 DAHP synthases, AroF is feedback-inhibited
CC by Tyr. The other 2 DAHP synthases are Phe- and Trp-sensitive,
CC respectively.
CC -!- SIMILARITY: Belongs to the class-I DAHP synthase family. {ECO:0000305}.
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DR EMBL; M31302; AAA27030.1; -; Genomic_DNA.
DR EMBL; AE006468; AAL21559.1; -; Genomic_DNA.
DR PIR; A35253; A35253.
DR RefSeq; NP_461600.1; NC_003197.2.
DR RefSeq; WP_001168062.1; NC_003197.2.
DR AlphaFoldDB; P0A1B5; -.
DR SMR; P0A1B5; -.
DR STRING; 99287.STM2670; -.
DR PaxDb; P0A1B5; -.
DR EnsemblBacteria; AAL21559; AAL21559; STM2670.
DR GeneID; 1254193; -.
DR KEGG; stm:STM2670; -.
DR PATRIC; fig|99287.12.peg.2814; -.
DR HOGENOM; CLU_030903_0_1_6; -.
DR OMA; VMENVAN; -.
DR PhylomeDB; P0A1B5; -.
DR BioCyc; SENT99287:STM2670-MON; -.
DR UniPathway; UPA00053; UER00084.
DR Proteomes; UP000001014; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0003849; F:3-deoxy-7-phosphoheptulonate synthase activity; IBA:GO_Central.
DR GO; GO:0009073; P:aromatic amino acid family biosynthetic process; IBA:GO_Central.
DR GO; GO:0008652; P:cellular amino acid biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0009423; P:chorismate biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.20.20.70; -; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR006218; DAHP1/KDSA.
DR InterPro; IPR006219; DHAP_synth_1.
DR PANTHER; PTHR21225; PTHR21225; 1.
DR Pfam; PF00793; DAHP_synth_1; 1.
DR PIRSF; PIRSF001361; DAHP_synthase; 1.
DR TIGRFAMs; TIGR00034; aroFGH; 1.
PE 3: Inferred from homology;
KW Amino-acid biosynthesis; Aromatic amino acid biosynthesis;
KW Reference proteome; Transferase.
FT CHAIN 1..356
FT /note="Phospho-2-dehydro-3-deoxyheptonate aldolase, Tyr-
FT sensitive"
FT /id="PRO_0000140834"
FT CONFLICT 185
FT /note="G -> A (in Ref. 1; AAA27030)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 356 AA; 38713 MW; 51EC218FC47B30A4 CRC64;
MQKDALNNVR ITDEQVLMTP EQLKAAFPLS LAQEAQIAQS RGIISDIIAG RDPRLLVVCG
PCSIHDPETA LEYARRFKAL AAEVSDSLYL VMRVYFEKPR TTVGWKGLIN DPHMDGSFDV
EAGLKIARQL LVELVNMGLP LATEALDPNS PQYLGDLFSW SAIGARTTES QTHREMASGL
SMPVGFKNGT DGSLATAINA MRAAAQPHRF VGINQAGQVA LLQTQGNPHG HVILRGGKAP
NYSPADVAQC EKEMEQAGLR PSLMVDCSHG NSNKDYRRQP AVAESVVAQI KDGNRSIIGL
MIESNIHEGN QSSEQPRSEM KYGVSVTDAC ISWEMTDALL REIHKDLSGQ LAVRVA
