NQRA_VIBAL
ID NQRA_VIBAL Reviewed; 446 AA.
AC Q56586;
DT 14-AUG-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 25-MAY-2022, entry version 90.
DE RecName: Full=Na(+)-translocating NADH-quinone reductase subunit A {ECO:0000255|HAMAP-Rule:MF_00425, ECO:0000303|PubMed:9490015};
DE Short=Na(+)-NQR subunit A {ECO:0000255|HAMAP-Rule:MF_00425};
DE Short=Na(+)-translocating NQR subunit A {ECO:0000255|HAMAP-Rule:MF_00425};
DE EC=7.2.1.1 {ECO:0000255|HAMAP-Rule:MF_00425, ECO:0000269|PubMed:9490015};
DE AltName: Full=NQR complex subunit A {ECO:0000255|HAMAP-Rule:MF_00425};
DE AltName: Full=NQR-1 subunit A {ECO:0000255|HAMAP-Rule:MF_00425};
GN Name=nqrA {ECO:0000255|HAMAP-Rule:MF_00425, ECO:0000303|PubMed:7805867};
GN Synonyms=nqr1 {ECO:0000303|PubMed:9490015};
OS Vibrio alginolyticus.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=663;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-10.
RC STRAIN=NCIMB 11038 / LMG 3418;
RX PubMed=7805867; DOI=10.1016/0014-5793(94)01275-x;
RA Beattie P., Tan K., Bourne R.M., Leach D.R.F., Rich P.R., Ward F.B.;
RT "Cloning and sequencing of four structural genes for the Na(+)-
RT translocating NADH-ubiquinone oxidoreductase of Vibrio alginolyticus.";
RL FEBS Lett. 356:333-338(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Hayashi M., Unemoto T., Sugiyama A.;
RL Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP PROTEIN SEQUENCE OF 1-10, CATALYTIC ACTIVITY, AND SUBUNIT.
RX PubMed=9490015; DOI=10.1016/s0014-5793(98)00016-7;
RA Nakayama Y., Hayashi M., Unemoto T.;
RT "Identification of six subunits constituting Na+-translocating NADH-quinone
RT reductase from the marine Vibrio alginolyticus.";
RL FEBS Lett. 422:240-242(1998).
RN [4]
RP PROTEIN SEQUENCE OF 1-9 AND 334-340.
RX PubMed=7805866; DOI=10.1016/0014-5793(94)01274-1;
RA Hayashi M., Hirai K., Unemoto T.;
RT "Cloning of the Na(+)-translocating NADH-quinone reductase gene from the
RT marine bacterium Vibrio alginolyticus and the expression of the beta-
RT subunit in Escherichia coli.";
RL FEBS Lett. 356:330-332(1994).
RN [5]
RP INHIBITION OF ENZYMATIC ACTIVITY.
RX PubMed=10549856; DOI=10.1248/bpb.22.1064;
RA Nakayama Y., Hayashi M., Yoshikawa K., Mochida K., Unemoto T.;
RT "Inhibitor studies of a new antibiotic, korormicin, 2-n-heptyl-4-
RT hydroxyquinoline N-oxide and Ag+ toward the Na+-translocating NADH-quinone
RT reductase from the marine Vibrio alginolyticus.";
RL Biol. Pharm. Bull. 22:1064-1067(1999).
RN [6]
RP REVIEW.
RX PubMed=11248187; DOI=10.1016/s0005-2728(00)00275-9;
RA Hayashi M., Nakayama Y., Unemoto T.;
RT "Recent progress in the Na(+)-translocating NADH-quinone reductase from the
RT marine Vibrio alginolyticus.";
RL Biochim. Biophys. Acta 1505:37-44(2001).
RN [7]
RP REVIEW.
RX PubMed=11248188; DOI=10.1016/s0005-2728(00)00276-0;
RA Steuber J.;
RT "Na(+) translocation by bacterial NADH:quinone oxidoreductases: an
RT extension to the complex-I family of primary redox pumps.";
RL Biochim. Biophys. Acta 1505:45-56(2001).
CC -!- FUNCTION: NQR complex catalyzes the reduction of ubiquinone-1 to
CC ubiquinol by two successive reactions, coupled with the transport of
CC Na(+) ions from the cytoplasm to the periplasm. NqrA to NqrE are
CC probably involved in the second step, the conversion of ubisemiquinone
CC to ubiquinol. {ECO:0000255|HAMAP-Rule:MF_00425,
CC ECO:0000305|PubMed:11248187, ECO:0000305|PubMed:11248188}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ubiquinone + H(+) + n Na(+)(in) + NADH = a ubiquinol + n
CC Na(+)(out) + NAD(+); Xref=Rhea:RHEA:47748, Rhea:RHEA-COMP:9565,
CC Rhea:RHEA-COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389,
CC ChEBI:CHEBI:17976, ChEBI:CHEBI:29101, ChEBI:CHEBI:57540,
CC ChEBI:CHEBI:57945; EC=7.2.1.1; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_00425, ECO:0000269|PubMed:9490015};
CC -!- ACTIVITY REGULATION: This reaction is tightly coupled to the Na(+)
CC pumping activity and specifically requires Na(+) for activity.
CC Inhibited by korormicin and 2-N-heptyl-4-hydroxyquinoline N-oxide
CC (HQNO). {ECO:0000269|PubMed:10549856}.
CC -!- SUBUNIT: Composed of six subunits; NqrA, NqrB, NqrC, NqrD, NqrE and
CC NqrF. {ECO:0000255|HAMAP-Rule:MF_00425, ECO:0000269|PubMed:9490015}.
CC -!- SIMILARITY: Belongs to the NqrA family. {ECO:0000255|HAMAP-
CC Rule:MF_00425, ECO:0000305}.
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DR EMBL; Z37111; CAA85476.1; -; Genomic_DNA.
DR EMBL; AB008030; BAA22910.1; -; Genomic_DNA.
DR PIR; S51015; S51015.
DR AlphaFoldDB; Q56586; -.
DR SMR; Q56586; -.
DR STRING; 663.BAU10_10840; -.
DR TCDB; 3.D.5.1.1; the na(+)-translocating nadh:quinone dehydrogenase (na-ndh or nqr) family.
DR PRIDE; Q56586; -.
DR eggNOG; COG1726; Bacteria.
DR GO; GO:0016655; F:oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor; IDA:UniProtKB.
DR GO; GO:0006814; P:sodium ion transport; IEA:UniProtKB-UniRule.
DR HAMAP; MF_00425; NqrA; 1.
DR InterPro; IPR008703; NqrA.
DR InterPro; IPR022615; NqrA_C_domain.
DR PANTHER; PTHR37839; PTHR37839; 1.
DR Pfam; PF05896; NQRA; 1.
DR Pfam; PF11973; NQRA_SLBB; 1.
DR TIGRFAMs; TIGR01936; nqrA; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Ion transport; NAD; Sodium; Sodium transport;
KW Translocase; Transport; Ubiquinone.
FT CHAIN 1..446
FT /note="Na(+)-translocating NADH-quinone reductase subunit
FT A"
FT /id="PRO_0000214203"
FT CONFLICT 337
FT /note="W -> L (in Ref. 4; AA sequence)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 446 AA; 48623 MW; 6D65ACAA53FE515C CRC64;
MITIKKGLDL PIAGTPSQVI NDGKTIKKVA LLGEEYVGMR PTMHVRVGDE VKKAQVLFED
KKNPGVKFTA PAAGKVIEVN RGAKRVLQSV VIEVAGEEQV TFDKFEAAQL SGLDREVIKT
QLVDSGLWTA LRTRPFSKVP AIESSTKAIF VTAMDTNPLA AKPELIINEQ QEAFIAGLDI
LSALTEGKVY VCKSGTSLPR SSQSNVEEHV FDGPHPAGLA GTHMHFLYPV NAENVAWSIN
YQDVIAFGKL FLTGELYTDR VVSLAGPVVN NPRLVRTVIG ASLDDLTDNE LMPGEVRVIS
GSVLTGTHAT GPHAYLGRYH QQVSVLREGR EKELFGWAMP GKNKFSVTRS FLGHVFKGQL
FNMTTTTNGS DRSMVPIGNY ERVMPLDMEP TLLLRDLCAG DTDSAQALGA LELDEEDLAL
CTFVCPGKYE YGTLLRECLD TIEKEG
