NQRA_VIBC3
ID NQRA_VIBC3 Reviewed; 446 AA.
AC A5F5X1;
DT 07-JAN-2015, integrated into UniProtKB/Swiss-Prot.
DT 07-JAN-2015, sequence version 2.
DT 25-MAY-2022, entry version 80.
DE RecName: Full=Na(+)-translocating NADH-quinone reductase subunit A {ECO:0000255|HAMAP-Rule:MF_00425};
DE Short=Na(+)-NQR subunit A {ECO:0000255|HAMAP-Rule:MF_00425};
DE Short=Na(+)-translocating NQR subunit A {ECO:0000255|HAMAP-Rule:MF_00425};
DE EC=7.2.1.1 {ECO:0000255|HAMAP-Rule:MF_00425, ECO:0000269|PubMed:11888296};
DE AltName: Full=NQR complex subunit A {ECO:0000255|HAMAP-Rule:MF_00425};
DE AltName: Full=NQR-1 subunit A {ECO:0000255|HAMAP-Rule:MF_00425};
GN Name=nqrA {ECO:0000255|HAMAP-Rule:MF_00425, ECO:0000303|PubMed:11888296};
GN OrderedLocusNames=VC0395_A1884 {ECO:0000312|EMBL:ABQ20722.1},
GN VC395_2411 {ECO:0000312|EMBL:ACP10401.1};
OS Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 /
OS O395).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=345073;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RA Heidelberg J.;
RL Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RX PubMed=19115014; DOI=10.1371/journal.pone.0004053;
RA Feng L., Reeves P.R., Lan R., Ren Y., Gao C., Zhou Z., Ren Y., Cheng J.,
RA Wang W., Wang J., Qian W., Li D., Wang L.;
RT "A recalibrated molecular clock and independent origins for the cholera
RT pandemic clones.";
RL PLoS ONE 3:E4053-E4053(2008).
RN [3]
RP CATALYTIC ACTIVITY, AND SUBUNIT.
RC STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RX PubMed=11888296; DOI=10.1021/bi011873o;
RA Barquera B., Hellwig P., Zhou W., Morgan J.E., Haese C.C., Gosink K.K.,
RA Nilges M., Bruesehoff P.J., Roth A., Lancaster C.R., Gennis R.B.;
RT "Purification and characterization of the recombinant Na(+)-translocating
RT NADH:quinone oxidoreductase from Vibrio cholerae.";
RL Biochemistry 41:3781-3789(2002).
RN [4]
RP SUBUNIT.
RC STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RX PubMed=20558724; DOI=10.1074/jbc.m109.071126;
RA Casutt M.S., Huber T., Brunisholz R., Tao M., Fritz G., Steuber J.;
RT "Localization and function of the membrane-bound riboflavin in the Na+-
RT translocating NADH:quinone oxidoreductase (Na+-NQR) from Vibrio cholerae.";
RL J. Biol. Chem. 285:27088-27099(2010).
CC -!- FUNCTION: NQR complex catalyzes the reduction of ubiquinone-1 to
CC ubiquinol by two successive reactions, coupled with the transport of
CC Na(+) ions from the cytoplasm to the periplasm. NqrA to NqrE are
CC probably involved in the second step, the conversion of ubisemiquinone
CC to ubiquinol. {ECO:0000255|HAMAP-Rule:MF_00425}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ubiquinone + H(+) + n Na(+)(in) + NADH = a ubiquinol + n
CC Na(+)(out) + NAD(+); Xref=Rhea:RHEA:47748, Rhea:RHEA-COMP:9565,
CC Rhea:RHEA-COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389,
CC ChEBI:CHEBI:17976, ChEBI:CHEBI:29101, ChEBI:CHEBI:57540,
CC ChEBI:CHEBI:57945; EC=7.2.1.1; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_00425, ECO:0000269|PubMed:11888296};
CC -!- SUBUNIT: Composed of six subunits; NqrA, NqrB, NqrC, NqrD, NqrE and
CC NqrF. {ECO:0000255|HAMAP-Rule:MF_00425, ECO:0000269|PubMed:11888296,
CC ECO:0000269|PubMed:20558724}.
CC -!- SIMILARITY: Belongs to the NqrA family. {ECO:0000255|HAMAP-
CC Rule:MF_00425, ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABQ20722.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=ACP10401.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; CP000627; ABQ20722.1; ALT_INIT; Genomic_DNA.
DR EMBL; CP001235; ACP10401.1; ALT_INIT; Genomic_DNA.
DR RefSeq; WP_001906078.1; NZ_JAACZH010000008.1.
DR AlphaFoldDB; A5F5X1; -.
DR SMR; A5F5X1; -.
DR STRING; 345073.VC395_2411; -.
DR EnsemblBacteria; ABQ20722; ABQ20722; VC0395_A1884.
DR GeneID; 57740916; -.
DR KEGG; vco:VC0395_A1884; -.
DR KEGG; vcr:VC395_2411; -.
DR PATRIC; fig|345073.21.peg.2324; -.
DR eggNOG; COG1726; Bacteria.
DR HOGENOM; CLU_046656_0_0_6; -.
DR BRENDA; 7.2.1.1; 15862.
DR Proteomes; UP000000249; Chromosome 2.
DR GO; GO:0016655; F:oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor; IDA:UniProtKB.
DR GO; GO:0006814; P:sodium ion transport; IDA:UniProtKB.
DR HAMAP; MF_00425; NqrA; 1.
DR InterPro; IPR008703; NqrA.
DR InterPro; IPR022615; NqrA_C_domain.
DR PANTHER; PTHR37839; PTHR37839; 1.
DR Pfam; PF05896; NQRA; 1.
DR Pfam; PF11973; NQRA_SLBB; 1.
DR TIGRFAMs; TIGR01936; nqrA; 1.
PE 1: Evidence at protein level;
KW Ion transport; NAD; Sodium; Sodium transport; Translocase; Transport;
KW Ubiquinone.
FT CHAIN 1..446
FT /note="Na(+)-translocating NADH-quinone reductase subunit
FT A"
FT /id="PRO_0000431654"
SQ SEQUENCE 446 AA; 48624 MW; 428E8C397EBA163D CRC64;
MITIKKGLDL PIAGTPSQVI SDGKAIKKVA LLGEEYVGMR PTMHVRVGDE VKKAQILFED
KKNPGVKFTS PVSGKVVEIN RGAKRVLQSV VIEVAGDDQV TFDKFEANQL ASLNRDAIKT
QLVESGLWTA FRTRPFSKVP AIDSTSEAIF VTAMDTNPLA AEPTVVINEQ SEAFVAGLDV
LSALTTGKVY VCKKGTSLPR SQQPNVEEHV FDGPHPAGLA GTHMHFLYPV SADHVAWSIN
YQDVIAVGQL FLTGELYTQR VVSLAGPVVN KPRLVRTVMG ASLEQLVDSE IMPGEVRIIS
GSVLSGTKAT GPHAYLGRYH LQVSVLREGR DKELFGWAMP GKNKFSVTRS FLGHLFKGQV
YNMTTTTNGS DRSMVPIGNY EKVMPLDMEP TLLLRDLCAG DSDSAVRLGA LELDEEDLAL
CTFVCPGKYE YGQLLRECLD KIEKEG
