AROG1_PETHY
ID AROG1_PETHY Reviewed; 533 AA.
AC A0A067XH53;
DT 02-DEC-2020, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2014, sequence version 1.
DT 03-AUG-2022, entry version 21.
DE RecName: Full=Phospho-2-dehydro-3-deoxyheptonate aldolase 1, chloroplastic {ECO:0000305};
DE EC=2.5.1.54 {ECO:0000250|UniProtKB:O53512};
DE AltName: Full=3-deoxy-D-arabino-heptulosonate 7-phosphate synthase 1 {ECO:0000303|PubMed:23275577, ECO:0000303|PubMed:24815009};
DE Short=DAHP synthase 1 {ECO:0000305};
DE Short=PhDAHP1 {ECO:0000303|PubMed:24815009};
DE AltName: Full=Phospho-2-keto-3-deoxyheptonate aldolase 1 {ECO:0000305};
DE Flags: Precursor;
GN Name=DAHP1 {ECO:0000303|PubMed:24815009};
GN Synonyms=DAHPS {ECO:0000303|PubMed:23275577},
GN DHS1 {ECO:0000303|PubMed:24815009};
OS Petunia hybrida (Petunia).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Petunioideae; Petunia.
OX NCBI_TaxID=4102;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE, TISSUE
RP SPECIFICITY, DEVELOPMENTAL STAGE, AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Mitchell; TISSUE=Corolla, and Leaf;
RX PubMed=24815009; DOI=10.1016/j.phytochem.2014.04.004;
RA Langer K.M., Jones C.R., Jaworski E.A., Rushing G.V., Kim J.Y., Clark D.G.,
RA Colquhoun T.A.;
RT "PhDAHP1 is required for floral volatile benzenoid/phenylpropanoid
RT biosynthesis in Petunia x hybrida cv 'Mitchell Diploid'.";
RL Phytochemistry 103:22-31(2014).
RN [2]
RP INDUCTION BY EOBI.
RC STRAIN=cv. W115;
RX PubMed=23275577; DOI=10.1105/tpc.112.105247;
RA Spitzer-Rimon B., Farhi M., Albo B., Cna'ani A., Ben Zvi M.M., Masci T.,
RA Edelbaum O., Yu Y., Shklarman E., Ovadis M., Vainstein A.;
RT "The R2R3-MYB-like regulatory factor EOBI, acting downstream of EOBII,
RT regulates scent production by activating ODO1 and structural scent-related
RT genes in petunia.";
RL Plant Cell 24:5089-5105(2012).
CC -!- FUNCTION: Involved in the production of volatile organic compounds
CC (VOCs), including floral volatile benzenoids and phenylpropanoids
CC (FVBP), in flowers of fragrant cultivars (e.g. cv. Mitchell and cv.
CC V26), scent attracting pollinators (e.g. the night-active hawkmoth
CC pollinator Manduca sexta) (PubMed:24815009). Catalyzes an aldol-like
CC condensation reaction between phosphoenolpyruvate (PEP) and D-erythrose
CC 4-phosphate (E4P) to generate 3-deoxy-D-arabino-heptulosonate 7-
CC phosphate (DAH7P) and inorganic phosphate (By similarity).
CC {ECO:0000250|UniProtKB:O53512, ECO:0000269|PubMed:24815009}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=D-erythrose 4-phosphate + H2O + phosphoenolpyruvate = 7-
CC phospho-2-dehydro-3-deoxy-D-arabino-heptonate + phosphate;
CC Xref=Rhea:RHEA:14717, ChEBI:CHEBI:15377, ChEBI:CHEBI:16897,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:58394, ChEBI:CHEBI:58702; EC=2.5.1.54;
CC Evidence={ECO:0000250|UniProtKB:O53512};
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000250|UniProtKB:O53512};
CC Note=Binds 1 divalent metal cation per subunit that could be manganese.
CC {ECO:0000250|UniProtKB:O53512};
CC -!- PATHWAY: Metabolic intermediate biosynthesis; chorismate biosynthesis;
CC chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step
CC 1/7. {ECO:0000250|UniProtKB:O53512}.
CC -!- SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:O53512}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000269|PubMed:24815009}.
CC -!- TISSUE SPECIFICITY: Mostly expressed in flowers, especially in petal
CC limbs and tubes, and, to a lower extent, in roots, stems, stigmas,
CC anthers, leaves and sepals. {ECO:0000269|PubMed:24815009}.
CC -!- DEVELOPMENTAL STAGE: During floral development, accumulates to highest
CC levels during open flower stages. {ECO:0000269|PubMed:24815009}.
CC -!- INDUCTION: Triggered by EOBI in flowers. {ECO:0000269|PubMed:23275577}.
CC -!- DISRUPTION PHENOTYPE: Reduced floral volatile benzenoids and
CC phenylpropanoids (FVBP) emission. {ECO:0000269|PubMed:24815009}.
CC -!- SIMILARITY: Belongs to the class-II DAHP synthase family.
CC {ECO:0000305}.
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DR EMBL; JQ955569; AFL02467.1; -; mRNA.
DR AlphaFoldDB; A0A067XH53; -.
DR SMR; A0A067XH53; -.
DR UniPathway; UPA00053; UER00084.
DR GO; GO:0009507; C:chloroplast; IDA:UniProtKB.
DR GO; GO:0003849; F:3-deoxy-7-phosphoheptulonate synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0009073; P:aromatic amino acid family biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0008652; P:cellular amino acid biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0009423; P:chorismate biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0010597; P:green leaf volatile biosynthetic process; IMP:UniProtKB.
DR Gene3D; 3.20.20.70; -; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR002480; DAHP_synth_2.
DR PANTHER; PTHR21337; PTHR21337; 1.
DR Pfam; PF01474; DAHP_synth_2; 1.
DR TIGRFAMs; TIGR01358; DAHP_synth_II; 1.
PE 2: Evidence at transcript level;
KW Amino-acid biosynthesis; Aromatic amino acid biosynthesis; Chloroplast;
KW Manganese; Metal-binding; Plastid; Transferase; Transit peptide.
FT TRANSIT 1..57
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 58..533
FT /note="Phospho-2-dehydro-3-deoxyheptonate aldolase 1,
FT chloroplastic"
FT /id="PRO_0000451509"
FT REGION 47..70
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 145
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250|UniProtKB:O53512"
FT BINDING 184
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:O53512"
FT BINDING 343..344
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:O53512"
FT BINDING 366
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:O53512"
FT BINDING 397
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:O53512"
FT BINDING 429
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250|UniProtKB:O53512"
FT BINDING 471
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250|UniProtKB:O53512"
FT BINDING 501
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250|UniProtKB:O53512"
SQ SEQUENCE 533 AA; 58894 MW; 16E7611033E00C01 CRC64;
MALSTNSTTS SLLPKTPLVQ QPLLKNASLP TTTKAIRFIQ PISAIHSSDS SKNTPIVSAK
PSSPPAATST AAATAVTKQE WSIDSWKTKK ALQLPEYPNQ EELKNVLKTI EDFPPIVFAG
EARHLEEKLG EAAMGRAFLL QGGDCAESFK EFNANNIRDT FRILLQMGAV LMFGGQMPVI
KVGRMAGQFA KPRSDNFEEK NGVKLPSYRG DNVNGDAFDL KSRTPDPQRL IRAYCQSAAT
LNLLRAFATG GYAAMQRVTQ WNLDFTEHSE QGDRYRELAN RVDEALGFMN AAGLTTDHPI
MTTTEFWTSH ECLLLPYEQS LTRLDSTSGL YYDCSAHFLW VGERTRQLDG AHVEFLRGIA
NPLGIKVSDK MDPSALVKLI EILNPQNKAG RITIITRMGA ENMRVKLPHL IRAVRGAGQI
VTWVSDPMHG NTIKAPCGLK TRPFDSIRAE VRAFFDVHEQ EGSHPGGVHL EMTGQNVTEC
IGGSRTVTFD DLSSRYHTHC DPRLNASQSL ELAFIIAERL RKRRLGSQSV LGQ
