NR0B1_PIG
ID NR0B1_PIG Reviewed; 471 AA.
AC P79386; O46520;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 2.
DT 25-MAY-2022, entry version 137.
DE RecName: Full=Nuclear receptor subfamily 0 group B member 1;
DE AltName: Full=Nuclear receptor DAX-1;
GN Name=NR0B1; Synonyms=AHCH, DAX1;
OS Sus scrofa (Pig).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX NCBI_TaxID=9823;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Adrenal gland;
RX PubMed=9453240; DOI=10.1016/s0303-7207(97)00189-5;
RA Parma P., Pailhoux E., Puissant C., Cotinot C.;
RT "Porcine Dax-1 gene: isolation and expression during gonadal development.";
RL Mol. Cell. Endocrinol. 135:49-58(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], AND VARIANT ALLELE 21BPDEL 352-HIS--GLU-358
RP DEL.
RC TISSUE=Testis;
RA Parma P., Pailhoux E., Cotinot C.;
RL Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-390.
RA Behdjani R., Silversides D.W.;
RL Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Orphan nuclear receptor. Component of a cascade required for
CC the development of the hypothalamic-pituitary-adrenal-gonadal axis.
CC Acts as a coregulatory protein that inhibits the transcriptional
CC activity of other nuclear receptors through heterodimeric interactions.
CC May also have a role in the development of the embryo and in the
CC maintenance of embryonic stem cell pluripotency (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Homodimer. Interacts with NR5A1, NR5A2, NR0B2 and with COPS2
CC (By similarity). Interacts with ESRRB; represses ESRRB activity at the
CC GATA6 promoter (By similarity). {ECO:0000250,
CC ECO:0000250|UniProtKB:Q61066}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Cytoplasm {ECO:0000250}.
CC Note=Shuttles between the cytoplasm and nucleus. Homodimers exits in
CC the cytoplasm and in the nucleus (By similarity). {ECO:0000250}.
CC -!- DOMAIN: Homodimerization involved an interaction between amino and
CC carboxy termini involving LXXLL motifs and steroid binding domain (AF-2
CC motif). Heterodimerizes with NR5A1 and NROB2 through its N-terminal
CC LXXLL motifs (By similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the nuclear hormone receptor family. NR0
CC subfamily. {ECO:0000305}.
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DR EMBL; U82466; AAB81101.1; -; mRNA.
DR EMBL; AF035816; AAC04761.1; -; mRNA.
DR EMBL; AF019044; AAB70254.1; -; Genomic_DNA.
DR RefSeq; NP_999552.1; NM_214387.1.
DR AlphaFoldDB; P79386; -.
DR SMR; P79386; -.
DR STRING; 9823.ENSSSCP00000012986; -.
DR PaxDb; P79386; -.
DR GeneID; 397680; -.
DR KEGG; ssc:397680; -.
DR CTD; 190; -.
DR eggNOG; KOG3575; Eukaryota.
DR HOGENOM; CLU_674314_0_0_1; -.
DR InParanoid; P79386; -.
DR OrthoDB; 955476at2759; -.
DR Proteomes; UP000008227; Unplaced.
DR Proteomes; UP000314985; Unplaced.
DR Genevisible; P79386; SS.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0016020; C:membrane; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; ISS:HGNC-UCL.
DR GO; GO:0042788; C:polysomal ribosome; ISS:HGNC-UCL.
DR GO; GO:0032448; F:DNA hairpin binding; ISS:HGNC-UCL.
DR GO; GO:0016922; F:nuclear receptor binding; ISS:UniProtKB.
DR GO; GO:0019904; F:protein domain specific binding; ISS:UniProtKB.
DR GO; GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
DR GO; GO:0003723; F:RNA binding; ISS:HGNC-UCL.
DR GO; GO:0003714; F:transcription corepressor activity; IBA:GO_Central.
DR GO; GO:0030325; P:adrenal gland development; ISS:HGNC-UCL.
DR GO; GO:0008406; P:gonad development; ISS:HGNC-UCL.
DR GO; GO:0008584; P:male gonad development; ISS:UniProtKB.
DR GO; GO:0043433; P:negative regulation of DNA-binding transcription factor activity; IBA:GO_Central.
DR GO; GO:0033144; P:negative regulation of intracellular steroid hormone receptor signaling pathway; ISS:UniProtKB.
DR GO; GO:0010894; P:negative regulation of steroid biosynthetic process; ISS:HGNC-UCL.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISS:HGNC-UCL.
DR GO; GO:0008104; P:protein localization; ISS:UniProtKB.
DR GO; GO:0007283; P:spermatogenesis; IBA:GO_Central.
DR Gene3D; 1.10.565.10; -; 1.
DR InterPro; IPR035500; NHR-like_dom_sf.
DR InterPro; IPR033544; NR0B1/2.
DR InterPro; IPR000536; Nucl_hrmn_rcpt_lig-bd.
DR InterPro; IPR001723; Nuclear_hrmn_rcpt.
DR InterPro; IPR025900; Nuclear_receptor_repeat.
DR PANTHER; PTHR24081; PTHR24081; 3.
DR Pfam; PF00104; Hormone_recep; 1.
DR Pfam; PF14046; NR_Repeat; 4.
DR PRINTS; PR00398; STRDHORMONER.
DR SMART; SM00430; HOLI; 1.
DR SUPFAM; SSF48508; SSF48508; 1.
DR PROSITE; PS51843; NR_LBD; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Nucleus; Receptor; Reference proteome; Repeat; Repressor;
KW Transcription; Transcription regulation.
FT CHAIN 1..471
FT /note="Nuclear receptor subfamily 0 group B member 1"
FT /id="PRO_0000053750"
FT REPEAT 1..67
FT /note="1"
FT REPEAT 68..134
FT /note="2"
FT REPEAT 135..201
FT /note="3"
FT REPEAT 202..253
FT /note="4; truncated"
FT DOMAIN 210..470
FT /note="NR LBD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01189"
FT REGION 1..253
FT /note="4 X 67 AA tandem repeats"
FT MOTIF 13..17
FT /note="LXXLL motif 1"
FT MOTIF 80..84
FT /note="LXXLL motif 2"
FT MOTIF 147..151
FT /note="LXXLL motif 3"
FT MOTIF 462..467
FT /note="AF-2 motif"
FT VARIANT 352..358
FT /note="Missing (in allele 21BPDel)"
FT /evidence="ECO:0000269|Ref.2"
FT CONFLICT 124..125
FT /note="GR -> AG (in Ref. 3; AAB70254)"
FT /evidence="ECO:0000305"
FT CONFLICT 243..244
FT /note="AQ -> QR (in Ref. 3; AAB70254)"
FT /evidence="ECO:0000305"
FT CONFLICT 266
FT /note="L -> V (in Ref. 3; AAB70254)"
FT /evidence="ECO:0000305"
FT CONFLICT 289
FT /note="S -> T (in Ref. 3; AAB70254)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 471 AA; 52128 MW; 8EE7D133A4677950 CRC64;
MAGEDHQWQG SILYNMLMSA KQTHATREAP EARLRGSCWG CSCGSEPPVG REGQPGGPAV
ALLYRCCFCG EDHPRQGSIL YNMLTSAKQT QETPEAPEAR LGGACWGCSC GSEPRVGREE
LPGGRATVLL YRCCFCGEEH PRQGSILYSL LTSAKQTHVA LEAPEARPGG AWWDRSYCAQ
RLGAREELPG GRPVTLPYRC CFCGEDHPRQ SGILCNMPMS AKQTHVAPEA QPGAPWWDPS
CAAQRVALKS PQVVCEAASA GLLKTLRFVK YLPCFQVLPL DQQLVLVRSC WAPLLMLELA
QDRLNFETVE TLEPSLLQMI LTTRRQETEG DEPPSPQPPV QPHLVLPSEA EHLPSVAEVQ
AIKGFLAKCW SLDISTKEYA YLKGTVLFNP DLPGLQCVKY IQGLQWGTQQ ILSEHIRMTH
RGYQARFAEL NSALFLLRFI NANVLAELFF RPIIGTVSMD DMMLEMLCAK L
