NR0B1_RAT
ID NR0B1_RAT Reviewed; 472 AA.
AC P70503; Q63152;
DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 25-MAY-2022, entry version 155.
DE RecName: Full=Nuclear receptor subfamily 0 group B member 1;
DE AltName: Full=Nuclear receptor DAX-1;
GN Name=Nr0b1; Synonyms=Ahch, Dax1;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Testis;
RX PubMed=8754790; DOI=10.1210/endo.137.8.8754790;
RA Majdic G., Saunders P.T.K.;
RT "Differential patterns of expression of DAX-1 and steroidogenic factor-1
RT (SF-1) in the fetal rat testis.";
RL Endocrinology 137:3586-3589(1996).
CC -!- FUNCTION: Orphan nuclear receptor. Component of a cascade required for
CC the development of the hypothalamic-pituitary-adrenal-gonadal axis.
CC Acts as a coregulatory protein that inhibits the transcriptional
CC activity of other nuclear receptors through heterodimeric interactions.
CC May also have a role in the development of the embryo and in the
CC maintenance of embryonic stem cell pluripotency (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Homodimer. Interacts with NR5A1, NR5A2, NR0B2 and with COPS2
CC (By similarity). Interacts with ESRRB; represses ESRRB activity at the
CC GATA6 promoter (By similarity). {ECO:0000250,
CC ECO:0000250|UniProtKB:Q61066}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Cytoplasm {ECO:0000250}.
CC Note=Shuttles between the cytoplasm and nucleus. Homodimers exits in
CC the cytoplasm and in the nucleus (By similarity). {ECO:0000250}.
CC -!- DOMAIN: Homodimerization involved an interaction between amino and
CC carboxy termini involving LXXLL motifs and steroid binding domain (AF-2
CC motif). Heterodimerizes with NR5A1 and NROB2 through its N-terminal
CC LXXLL motifs (By similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the nuclear hormone receptor family. NR0
CC subfamily. {ECO:0000305}.
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DR EMBL; X99470; CAA67832.1; -; mRNA.
DR RefSeq; NP_445769.1; NM_053317.1.
DR STRING; 10116.ENSRNOP00000005023; -.
DR PhosphoSitePlus; P70503; -.
DR PaxDb; P70503; -.
DR GeneID; 58850; -.
DR KEGG; rno:58850; -.
DR CTD; 190; -.
DR RGD; 62028; Nr0b1.
DR eggNOG; KOG3575; Eukaryota.
DR InParanoid; P70503; -.
DR OrthoDB; 955476at2759; -.
DR PhylomeDB; P70503; -.
DR Reactome; R-RNO-383280; Nuclear Receptor transcription pathway.
DR PRO; PR:P70503; -.
DR Proteomes; UP000002494; Unplaced.
DR GO; GO:0005737; C:cytoplasm; IDA:RGD.
DR GO; GO:0016020; C:membrane; ISO:RGD.
DR GO; GO:0005634; C:nucleus; IDA:RGD.
DR GO; GO:0042788; C:polysomal ribosome; ISS:HGNC-UCL.
DR GO; GO:0003677; F:DNA binding; TAS:RGD.
DR GO; GO:0032448; F:DNA hairpin binding; ISS:HGNC-UCL.
DR GO; GO:0003690; F:double-stranded DNA binding; IMP:RGD.
DR GO; GO:0016922; F:nuclear receptor binding; ISO:RGD.
DR GO; GO:0019904; F:protein domain specific binding; ISO:RGD.
DR GO; GO:0042803; F:protein homodimerization activity; ISO:RGD.
DR GO; GO:0003723; F:RNA binding; ISS:HGNC-UCL.
DR GO; GO:0061629; F:RNA polymerase II-specific DNA-binding transcription factor binding; ISO:RGD.
DR GO; GO:0043565; F:sequence-specific DNA binding; IMP:RGD.
DR GO; GO:0003714; F:transcription corepressor activity; ISO:RGD.
DR GO; GO:0030325; P:adrenal gland development; ISS:HGNC-UCL.
DR GO; GO:0030154; P:cell differentiation; ISO:RGD.
DR GO; GO:0008406; P:gonad development; ISS:HGNC-UCL.
DR GO; GO:0033327; P:Leydig cell differentiation; ISO:RGD.
DR GO; GO:0008584; P:male gonad development; ISO:RGD.
DR GO; GO:0030238; P:male sex determination; ISO:RGD.
DR GO; GO:0043433; P:negative regulation of DNA-binding transcription factor activity; IMP:RGD.
DR GO; GO:0033144; P:negative regulation of intracellular steroid hormone receptor signaling pathway; ISO:RGD.
DR GO; GO:0010894; P:negative regulation of steroid biosynthetic process; ISS:HGNC-UCL.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IMP:RGD.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISS:HGNC-UCL.
DR GO; GO:0008104; P:protein localization; ISO:RGD.
DR GO; GO:0050810; P:regulation of steroid biosynthetic process; TAS:RGD.
DR GO; GO:0035902; P:response to immobilization stress; ISO:RGD.
DR GO; GO:0010033; P:response to organic substance; IEP:RGD.
DR GO; GO:0043434; P:response to peptide hormone; IEP:RGD.
DR GO; GO:0060008; P:Sertoli cell differentiation; ISO:RGD.
DR GO; GO:0007530; P:sex determination; ISO:RGD.
DR GO; GO:0007283; P:spermatogenesis; IEP:RGD.
DR Gene3D; 1.10.565.10; -; 1.
DR InterPro; IPR035500; NHR-like_dom_sf.
DR InterPro; IPR033544; NR0B1/2.
DR InterPro; IPR000536; Nucl_hrmn_rcpt_lig-bd.
DR InterPro; IPR001723; Nuclear_hrmn_rcpt.
DR InterPro; IPR025900; Nuclear_receptor_repeat.
DR PANTHER; PTHR24081; PTHR24081; 1.
DR Pfam; PF00104; Hormone_recep; 1.
DR Pfam; PF14046; NR_Repeat; 4.
DR PRINTS; PR00398; STRDHORMONER.
DR SMART; SM00430; HOLI; 1.
DR SUPFAM; SSF48508; SSF48508; 1.
DR PROSITE; PS51843; NR_LBD; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Nucleus; Receptor; Reference proteome; Repeat; Repressor;
KW Transcription; Transcription regulation.
FT CHAIN 1..472
FT /note="Nuclear receptor subfamily 0 group B member 1"
FT /id="PRO_0000053751"
FT REPEAT 1..67
FT /note="1"
FT REPEAT 68..135
FT /note="2"
FT REPEAT 136..202
FT /note="3"
FT DOMAIN 190..471
FT /note="NR LBD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01189"
FT REPEAT 203..255
FT /note="4; truncated"
FT REGION 1..255
FT /note="4 X 67 AA tandem repeats"
FT REGION 215..239
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 322..345
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 13..17
FT /note="LXXLL motif 1"
FT MOTIF 80..84
FT /note="LXXLL motif 2"
FT MOTIF 148..152
FT /note="LXXLL motif 3"
FT MOTIF 463..468
FT /note="AF-2 motif"
SQ SEQUENCE 472 AA; 52756 MW; F9E0BBA1AFF55196 CRC64;
MAGEDHPWHG SILYNLLMSA KQKHGSREER EVRLGAQCWG CACGTQPVLG GEGLPGGQAL
SLLYRCCFCG ENHPRQGGIL YSMLTNARQP SGATEAPRAR FRTPCWGCAC SNAKPLVGRX
GLPAGQVPSL LYRCCFCGKK HPRQGSILYS LLTNAQQTHV SREVPEAHRG GEWWQLSYCT
HNVGGPEGLQ STQAMAFLYR SYVCCEEQPQ QSSVASDTPV RADQTPAAPQ EQPRAPWWDT
SSGVQRPIAL KDPQVVCEAA SAGLLKTLRF VKYLPCFQIL PLDQQLVLVR SCWAPLLMLE
LAQDHLHFEM MEISEPNLMH EMLTTRRQET EGPEPADPQA TEQPQTVSAE AGHVLSVAAV
QAIKSFFFKC WSLNIDTKEY AYLKGTVLFN PDLPGLQCVK YIESLQWRTQ QILTEHIRLM
QREYQIRSAE LNSALFLLRF INTDVVTELF FRPIIGAVSM DDMMLEMLCA KL
