NR6A1_MOUSE
ID NR6A1_MOUSE Reviewed; 495 AA.
AC Q64249; A2AQL8; A2AQM1; Q687Z3;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 181.
DE RecName: Full=Nuclear receptor subfamily 6 group A member 1;
DE AltName: Full=Germ cell nuclear factor;
DE Short=GCNF;
DE Short=mGCNF;
DE AltName: Full=Retinoid receptor-related testis-specific receptor;
DE Short=RTR;
GN Name=Nr6a1; Synonyms=Gcnf;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, DNA-BINDING,
RP HOMODIMERIZATION, AND TISSUE SPECIFICITY.
RC STRAIN=BALB/cJ; TISSUE=Testis;
RX PubMed=7854358; DOI=10.1210/mend.8.10.7854358;
RA Chen F., Cooney A.J., Wang Y., Law S.W., O'Malley B.W.;
RT "Cloning of a novel orphan receptor (GCNF) expressed during germ cell
RT development.";
RL Mol. Endocrinol. 8:1434-1444(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND TISSUE SPECIFICITY.
RC STRAIN=CD-1; TISSUE=Testis;
RX PubMed=7835709; DOI=10.1016/0378-1119(94)00656-d;
RA Hirose T., O'Brien D.A., Jetten A.M.;
RT "RTR: a new member of the nuclear receptor superfamily that is highly
RT expressed in murine testis.";
RL Gene 152:247-251(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=129/SvJ; TISSUE=Liver;
RX PubMed=11178239; DOI=10.1186/gb-2000-1-3-research0006;
RA Suesens U., Borgmeyer U.;
RT "Genomic structure of the gene for mouse germ cell nuclear factor (GCNF).";
RL Genome Biol. 1:RESEARCH0006.1-RESEARCH0006.3(2000).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=129/Ola; TISSUE=Testis;
RX PubMed=12606326; DOI=10.1095/biolreprod.102.012013;
RA Yang G., Zhang Y.-L., Buchold G.M., Jetten A.M., O'Brien D.A.;
RT "Analysis of germ cell nuclear factor transcripts and protein expression
RT during spermatogenesis.";
RL Biol. Reprod. 68:1620-1630(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [6]
RP INTERACTION WITH UIMC1.
RX PubMed=7760852; DOI=10.1210/mend.9.1.7760852;
RA Seol W., Choi H.S., Moore D.D.;
RT "Isolation of proteins that interact specifically with the retinoid X
RT receptor: two novel orphan receptors.";
RL Mol. Endocrinol. 9:72-85(1995).
RN [7]
RP DNA-BINDING, AND HOMODIMERIZATION.
RX PubMed=9025060; DOI=10.1016/s0925-4773(96)00599-0;
RA Joos T.O., David R., Dreyer C.;
RT "xGCNF, a nuclear orphan receptor is expressed during neurulation in
RT Xenopus laevis.";
RL Mech. Dev. 60:45-57(1996).
RN [8]
RP INTERACTION WITH UIMC1, AND MUTAGENESIS OF 246-SER-TYR-247; 254-LEU-PRO-255
RP AND 265-SER-TYR-266.
RX PubMed=12080054; DOI=10.1074/jbc.m203475200;
RA Yan Z., Kim Y.-S., Jetten A.M.;
RT "RAP80: a novel nuclear protein that interacts with the retinoid-related
RT testis-associated receptor.";
RL J. Biol. Chem. 277:32379-32388(2002).
CC -!- FUNCTION: Orphan nuclear receptor. Binds to a response element
CC containing the sequence 5'-TCAAGGTCA-3'. May be involved in the
CC regulation of gene expression in germ cell development during
CC gametogenesis. {ECO:0000269|PubMed:7854358}.
CC -!- SUBUNIT: Homodimer. Interacts with UIMC1. {ECO:0000269|PubMed:12080054,
CC ECO:0000269|PubMed:7760852}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q64249-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q64249-2; Sequence=VSP_025929, VSP_025931;
CC Name=3;
CC IsoId=Q64249-3; Sequence=VSP_025928, VSP_025930;
CC -!- TISSUE SPECIFICITY: Expressed in the germ cells of both the adult
CC testis and ovary, being most abundant in spermatids.
CC {ECO:0000269|PubMed:7835709, ECO:0000269|PubMed:7854358}.
CC -!- SIMILARITY: Belongs to the nuclear hormone receptor family. NR6
CC subfamily. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U14666; AAA92682.1; -; mRNA.
DR EMBL; U09563; AAA64746.1; -; mRNA.
DR EMBL; AF254821; AAG24485.1; -; Genomic_DNA.
DR EMBL; AF254575; AAG24485.1; JOINED; Genomic_DNA.
DR EMBL; AF254815; AAG24485.1; JOINED; Genomic_DNA.
DR EMBL; AF254816; AAG24485.1; JOINED; Genomic_DNA.
DR EMBL; AF254817; AAG24485.1; JOINED; Genomic_DNA.
DR EMBL; AF254818; AAG24485.1; JOINED; Genomic_DNA.
DR EMBL; AF254819; AAG24485.1; JOINED; Genomic_DNA.
DR EMBL; AF254820; AAG24485.1; JOINED; Genomic_DNA.
DR EMBL; AF390896; AAM55193.1; -; mRNA.
DR EMBL; AF390900; AAM95878.1; -; Genomic_DNA.
DR EMBL; AF390897; AAM95878.1; JOINED; Genomic_DNA.
DR EMBL; AF390898; AAM95878.1; JOINED; Genomic_DNA.
DR EMBL; AF390899; AAM95878.1; JOINED; Genomic_DNA.
DR EMBL; AL844842; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL935155; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS16012.1; -. [Q64249-1]
DR CCDS; CCDS50581.1; -. [Q64249-3]
DR PIR; A57053; A57053.
DR RefSeq; NP_001153020.1; NM_001159548.1. [Q64249-3]
DR RefSeq; NP_034394.1; NM_010264.4. [Q64249-1]
DR PDB; 5KRB; X-ray; 2.10 A; B/G=72-155.
DR PDBsum; 5KRB; -.
DR AlphaFoldDB; Q64249; -.
DR SMR; Q64249; -.
DR BioGRID; 199868; 16.
DR STRING; 10090.ENSMUSP00000108498; -.
DR PhosphoSitePlus; Q64249; -.
DR PaxDb; Q64249; -.
DR PRIDE; Q64249; -.
DR ProteomicsDB; 252849; -. [Q64249-1]
DR ProteomicsDB; 252850; -. [Q64249-2]
DR Antibodypedia; 16337; 336 antibodies from 35 providers.
DR DNASU; 14536; -.
DR Ensembl; ENSMUST00000076275; ENSMUSP00000075624; ENSMUSG00000063972. [Q64249-1]
DR Ensembl; ENSMUST00000112877; ENSMUSP00000108498; ENSMUSG00000063972. [Q64249-1]
DR Ensembl; ENSMUST00000168098; ENSMUSP00000126009; ENSMUSG00000063972. [Q64249-3]
DR GeneID; 14536; -.
DR KEGG; mmu:14536; -.
DR UCSC; uc012bus.1; mouse. [Q64249-3]
DR UCSC; uc012buu.1; mouse. [Q64249-1]
DR CTD; 2649; -.
DR MGI; MGI:1352459; Nr6a1.
DR VEuPathDB; HostDB:ENSMUSG00000063972; -.
DR eggNOG; KOG3575; Eukaryota.
DR GeneTree; ENSGT00940000157936; -.
DR HOGENOM; CLU_007368_6_1_1; -.
DR InParanoid; Q64249; -.
DR OMA; SPEAYVC; -.
DR OrthoDB; 619653at2759; -.
DR PhylomeDB; Q64249; -.
DR TreeFam; TF350737; -.
DR Reactome; R-MMU-383280; Nuclear Receptor transcription pathway.
DR BioGRID-ORCS; 14536; 5 hits in 74 CRISPR screens.
DR ChiTaRS; Nr6a1; mouse.
DR PRO; PR:Q64249; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q64249; protein.
DR Bgee; ENSMUSG00000063972; Expressed in primitive streak and 185 other tissues.
DR ExpressionAtlas; Q64249; baseline and differential.
DR Genevisible; Q64249; MM.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0005667; C:transcription regulator complex; IDA:MGI.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:NTNU_SB.
DR GO; GO:0004879; F:nuclear receptor activity; IBA:GO_Central.
DR GO; GO:0042803; F:protein homodimerization activity; IDA:UniProtKB.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:MGI.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0007276; P:gamete generation; IEP:UniProtKB.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:MGI.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:NTNU_SB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0007283; P:spermatogenesis; IEA:UniProtKB-KW.
DR Gene3D; 1.10.565.10; -; 1.
DR Gene3D; 3.30.50.10; -; 1.
DR InterPro; IPR035500; NHR-like_dom_sf.
DR InterPro; IPR000536; Nucl_hrmn_rcpt_lig-bd.
DR InterPro; IPR001723; Nuclear_hrmn_rcpt.
DR InterPro; IPR001628; Znf_hrmn_rcpt.
DR InterPro; IPR013088; Znf_NHR/GATA.
DR Pfam; PF00104; Hormone_recep; 1.
DR Pfam; PF00105; zf-C4; 1.
DR PRINTS; PR00398; STRDHORMONER.
DR PRINTS; PR00047; STROIDFINGER.
DR SMART; SM00430; HOLI; 1.
DR SMART; SM00399; ZnF_C4; 1.
DR SUPFAM; SSF48508; SSF48508; 1.
DR PROSITE; PS51843; NR_LBD; 1.
DR PROSITE; PS00031; NUCLEAR_REC_DBD_1; 1.
DR PROSITE; PS51030; NUCLEAR_REC_DBD_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Developmental protein; Differentiation;
KW DNA-binding; Metal-binding; Nucleus; Receptor; Reference proteome;
KW Spermatogenesis; Transcription; Transcription regulation; Zinc;
KW Zinc-finger.
FT CHAIN 1..495
FT /note="Nuclear receptor subfamily 6 group A member 1"
FT /id="PRO_0000053742"
FT DOMAIN 264..495
FT /note="NR LBD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01189"
FT DNA_BIND 72..147
FT /note="Nuclear receptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00407"
FT ZN_FING 75..95
FT /note="NR C4-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00407"
FT ZN_FING 111..135
FT /note="NR C4-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00407"
FT REGION 1..34
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 145..165
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 177..214
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 187..268
FT /note="Sufficient for interaction with UIMC1"
FT COMPBIAS 188..214
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..57
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000305"
FT /id="VSP_025928"
FT VAR_SEQ 48..62
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_025929"
FT VAR_SEQ 58..62
FT /note="QGHIP -> METWE (in isoform 3)"
FT /evidence="ECO:0000305"
FT /id="VSP_025930"
FT VAR_SEQ 63
FT /note="V -> I (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_025931"
FT MUTAGEN 246..247
FT /note="SY->GG: Abolishes interaction with RXRIP110."
FT /evidence="ECO:0000269|PubMed:12080054"
FT MUTAGEN 254..255
FT /note="LP->AA: Does not abolish interaction with RXRIP110."
FT /evidence="ECO:0000269|PubMed:12080054"
FT MUTAGEN 265..266
FT /note="SY->AA: Does not abolish interaction with RXRIP110."
FT /evidence="ECO:0000269|PubMed:12080054"
FT TURN 76..78
FT /evidence="ECO:0007829|PDB:5KRB"
FT STRAND 84..86
FT /evidence="ECO:0007829|PDB:5KRB"
FT HELIX 93..104
FT /evidence="ECO:0007829|PDB:5KRB"
FT TURN 121..123
FT /evidence="ECO:0007829|PDB:5KRB"
FT HELIX 124..126
FT /evidence="ECO:0007829|PDB:5KRB"
FT HELIX 128..138
FT /evidence="ECO:0007829|PDB:5KRB"
FT HELIX 142..144
FT /evidence="ECO:0007829|PDB:5KRB"
SQ SEQUENCE 495 AA; 55976 MW; ECB0AB3E99E42661 CRC64;
MERDERPPSG GGGGGGSAGF LEPPAALPPP PRNGFCQDEL AELDPGTNGE TDSLTLGQGH
IPVSVPDDRA EQRTCLICGD RATGLHYGII SCEGCKGFFK RSICNKRVYR CSRDKNCVMS
RKQRNRCQYC RLLKCLQMGM NRKAIREDGM PGGRNKSIGP VQISEEEIER IMSGQEFEEE
ANHWSNHGDS DHSSPGNRAS ESNQPSPGST LSSSRSVELN GFMAFRDQYM GMSVPPHYQY
IPHLFSYSGH SPLLPPQARS LDPQSYSLIH QLMSAEDLEP LGTPMLIEDG YAVTQAELFA
LLCRLADELL FRQIAWIKKL PFFCELSIKD YTCLLSSTWQ ELILLSSLTV YSKQIFGELA
DVTAKYSPSD EELHRFSDEG MEVIERLIYL YHKFHQLKVS NEEYACMKAI NFLNQDIRGL
TSASQLEQLN KRYWYICQDF TEYKYTHQPN RFPDLMMCLP EIRYIAGKMV NVPLEQLPLL
FKVVLHSCKT STVKE
