NR6A1_XENLA
ID NR6A1_XENLA Reviewed; 435 AA.
AC P70033; Q8JG52;
DT 29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 29-MAY-2007, sequence version 2.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Nuclear receptor subfamily 6 group A member 1;
DE AltName: Full=Germ cell nuclear factor;
DE Short=GCNF;
DE Short=xGCNF;
GN Name=nr6a1 {ECO:0000250|UniProtKB:Q15406};
GN Synonyms=gcnf {ECO:0000303|PubMed:9025060};
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAC60126.1}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM EM), FUNCTION, DNA-BINDING,
RP HOMODIMERIZATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RC TISSUE=Neurula {ECO:0000269|PubMed:9025060};
RX PubMed=9025060; DOI=10.1016/s0925-4773(96)00599-0;
RA Joos T.O., David R., Dreyer C.;
RT "xGCNF, a nuclear orphan receptor is expressed during neurulation in
RT Xenopus laevis.";
RL Mech. Dev. 60:45-57(1996).
RN [2] {ECO:0000305, ECO:0000312|EMBL:AAM95452.1}
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1-61 (ISOFORM OO), ALTERNATIVE SPLICING,
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC TISSUE=Ovary {ECO:0000312|EMBL:AAM95452.1};
RX PubMed=12351198; DOI=10.1016/s0925-4773(02)00281-2;
RA Schohl A., Barreto G., Joos T.O., Dreyer C.;
RT "Oocytes and embryos of Xenopus laevis express two different isoforms of
RT germ cell nuclear factor (GCNF, NR6A1).";
RL Mech. Dev. 118:261-264(2002).
RN [3] {ECO:0000305}
RP FUNCTION, DNA-BINDING, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RX PubMed=10349627; DOI=10.1016/s0925-4773(98)00181-6;
RA David R., Joos T.O., Dreyer C.;
RT "Anteroposterior patterning and organogenesis of Xenopus laevis require a
RT correct dose of germ cell nuclear factor (xGCNF).";
RL Mech. Dev. 79:137-152(1998).
RN [4] {ECO:0000305}
RP FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=10452854; DOI=10.1006/dbio.1999.9368;
RA Song K., Takemaru K., Moon R.T.;
RT "A role for xGCNF in midbrain-hindbrain patterning in Xenopus laevis.";
RL Dev. Biol. 213:170-179(1999).
RN [5] {ECO:0000305}
RP FUNCTION.
RX PubMed=12729562; DOI=10.1016/s0012-1606(03)00109-x;
RA Barreto G., Reintsch W., Kaufmann C., Dreyer C.;
RT "The function of Xenopus germ cell nuclear factor (xGCNF) in morphogenetic
RT movements during neurulation.";
RL Dev. Biol. 257:329-342(2003).
RN [6] {ECO:0000305}
RP FUNCTION, AND INDUCTION.
RX PubMed=12676320; DOI=10.1016/s0925-4773(03)00018-2;
RA Barreto G., Borgmeyer U., Dreyer C.;
RT "The germ cell nuclear factor is required for retinoic acid signaling
RT during Xenopus development.";
RL Mech. Dev. 120:415-428(2003).
CC -!- FUNCTION: Probable orphan nuclear receptor. Binds to a response element
CC containing repeats of the motif 5'-AGGTCA-3'. Required for anterior-
CC posterior patterning during organogenesis. Acts with chordin to play a
CC role in patterning the midbrain-hindbrain. Isoform Em is required for
CC integrin-mediated cell matrix interaction during neurulation and for
CC the morphogenetic movements leading to formation of the neural tube.
CC Also mediates the effect of retinoic acid on primary neurogenesis.
CC {ECO:0000269|PubMed:10349627, ECO:0000269|PubMed:10452854,
CC ECO:0000269|PubMed:12676320, ECO:0000269|PubMed:12729562,
CC ECO:0000269|PubMed:9025060}.
CC -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:9025060}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:10349627,
CC ECO:0000269|PubMed:9025060}. Nucleus {ECO:0000255|PROSITE-
CC ProRule:PRU00407, ECO:0000269|PubMed:10349627,
CC ECO:0000269|PubMed:9025060}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=Em {ECO:0000269|PubMed:9025060}; Synonyms=xlEm
CC {ECO:0000269|PubMed:12351198};
CC IsoId=P70033-1; Sequence=Displayed;
CC Name=Oo {ECO:0000269|PubMed:12351198}; Synonyms=xlOo
CC {ECO:0000269|PubMed:12351198};
CC IsoId=P70033-2; Sequence=VSP_052452;
CC -!- TISSUE SPECIFICITY: Transiently expressed in differentiating cells of
CC all embryonic germ layers. Expressed in an anterior to posterior
CC concentration gradient from late gastrula to midneurula stages. Shows a
CC complicated spatio-temporal pattern of expression during neurulation,
CC being predominant in the neural plate and neural crest in midneurula
CC embryos. At late tailbud (stage 30), mainly expressed in the head
CC mesenchyme, gill arches and tail tip. Expression persists in the
CC epidermis, somites and endoderm, and in the central nervous system,
CC expression is restricted to the midbrain, hindbrain and part of the
CC spinal cord. Isoforms Oo and Em are both expressed in the brain and
CC isoform Oo is expressed in the germ cells of both the adult testis and
CC ovary. {ECO:0000269|PubMed:10349627, ECO:0000269|PubMed:10452854,
CC ECO:0000269|PubMed:12351198, ECO:0000269|PubMed:9025060}.
CC -!- DEVELOPMENTAL STAGE: Isoform Oo is expressed both maternally and
CC zygotically, being predominantly expressed in oocytes and embryos
CC before the midblastula transition but present at later stages at very
CC low levels. Isoform Em is expressed zygotically between late gastrula
CC stage and late tailbud stage 30, being predominant during tailbud
CC stages. {ECO:0000269|PubMed:10349627, ECO:0000269|PubMed:10452854,
CC ECO:0000269|PubMed:12351198, ECO:0000269|PubMed:9025060}.
CC -!- INDUCTION: By retinoic acid. {ECO:0000269|PubMed:12676320}.
CC -!- MISCELLANEOUS: [Isoform Em]: Embryonic form.
CC {ECO:0000269|PubMed:12351198}.
CC -!- MISCELLANEOUS: [Isoform Oo]: Oocyte form.
CC {ECO:0000269|PubMed:12351198}.
CC -!- SIMILARITY: Belongs to the nuclear hormone receptor family. NR6
CC subfamily. {ECO:0000255}.
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DR EMBL; U58683; AAC60126.1; -; mRNA.
DR EMBL; AY130967; AAM95452.1; -; mRNA.
DR RefSeq; NP_001084102.1; NM_001090633.1.
DR AlphaFoldDB; P70033; -.
DR SMR; P70033; -.
DR GeneID; 399304; -.
DR KEGG; xla:399304; -.
DR CTD; 399304; -.
DR Xenbase; XB-GENE-487596; nr6a1.S.
DR OrthoDB; 619653at2759; -.
DR Proteomes; UP000186698; Chromosome 8S.
DR Bgee; 399304; Expressed in neurula embryo and 6 other tissues.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0042803; F:protein homodimerization activity; IDA:UniProtKB.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0048513; P:animal organ development; IMP:UniProtKB.
DR GO; GO:0009952; P:anterior/posterior pattern specification; IMP:UniProtKB.
DR GO; GO:0016477; P:cell migration; IMP:UniProtKB.
DR GO; GO:0030917; P:midbrain-hindbrain boundary development; IMP:UniProtKB.
DR GO; GO:0022008; P:neurogenesis; IMP:UniProtKB.
DR Gene3D; 1.10.565.10; -; 1.
DR Gene3D; 3.30.50.10; -; 1.
DR InterPro; IPR035500; NHR-like_dom_sf.
DR InterPro; IPR000536; Nucl_hrmn_rcpt_lig-bd.
DR InterPro; IPR001723; Nuclear_hrmn_rcpt.
DR InterPro; IPR001628; Znf_hrmn_rcpt.
DR InterPro; IPR013088; Znf_NHR/GATA.
DR Pfam; PF00104; Hormone_recep; 1.
DR Pfam; PF00105; zf-C4; 1.
DR PRINTS; PR00398; STRDHORMONER.
DR PRINTS; PR00047; STROIDFINGER.
DR SMART; SM00430; HOLI; 1.
DR SMART; SM00399; ZnF_C4; 1.
DR SUPFAM; SSF48508; SSF48508; 1.
DR PROSITE; PS51843; NR_LBD; 1.
DR PROSITE; PS00031; NUCLEAR_REC_DBD_1; 1.
DR PROSITE; PS51030; NUCLEAR_REC_DBD_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Developmental protein; Differentiation;
KW DNA-binding; Metal-binding; Neurogenesis; Nucleus; Receptor;
KW Reference proteome; Transcription; Transcription regulation; Zinc;
KW Zinc-finger.
FT CHAIN 1..435
FT /note="Nuclear receptor subfamily 6 group A member 1"
FT /id="PRO_0000289151"
FT DOMAIN 204..435
FT /note="NR LBD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01189"
FT DNA_BIND 11..86
FT /note="Nuclear receptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00407"
FT ZN_FING 14..34
FT /note="NR C4-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00407"
FT ZN_FING 50..69
FT /note="NR C4-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00407"
FT REGION 84..158
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 116..158
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..5
FT /note="MDTWE -> MEVERSTSGLMDSTGFYKEPAGETEHTAAGNMSDLSSDGIKIN
FT SFV (in isoform Oo)"
FT /evidence="ECO:0000303|PubMed:12351198"
FT /id="VSP_052452"
FT CONFLICT 41
FT /note="S -> T (in Ref. 1; AAC60126)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 435 AA; 50005 MW; B8DDF322E0686BA6 CRC64;
MDTWEDDRVD QRACLICGDR ATGLHYGIIS CEGCKGFFKR SICNKRVYRC SRDKNCVMSR
KQRNRCQYCR LLKCLQMGMN RKAIREDGMP GGRNKSIGPV QISDEEVERI MSGQEFEEEA
NTSWSNNGDS DHSSPGNAVS ESNQPSPVST PSSSRSMELN GFSSLRDQYL GTPGPTHYQY
LPHLFSFSAH PTIIPAQSRS LDPQSHTLIN QLLTAEDIEP LNTPMLIEDG YKVTQSELFA
LLCRLADELL FRQITWVKKL PFFCDLSIKD YTCLLSTTWQ ELILLSSITT YSKQIFGDLT
DVTSKYSPSE DELHRFSEDG MEVMERLIYL FRKFSQLKVS NEEYVCMKAI NFLNQDIQGI
SSASQVEQLN KRYWYVCQDF TEYRYPHQPN RFPDLMMCLP EVRYIAGKLV NVPLEQLPLL
FKAFLHSCKT IVTKE
