NR6A1_XENTR
ID NR6A1_XENTR Reviewed; 435 AA.
AC Q66JK1;
DT 29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Nuclear receptor subfamily 6 group A member 1;
DE AltName: Full=Germ cell nuclear factor;
DE Short=GCNF;
GN Name=nr6a1 {ECO:0000312|EMBL:CAJ83090.1};
GN Synonyms=gcnf {ECO:0000250|UniProtKB:P70033}; ORFNames=TNeu077g24.1;
OS Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX NCBI_TaxID=8364;
RN [1] {ECO:0000305, ECO:0000312|EMBL:CAJ83090.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM EM), AND NUCLEOTIDE
RP SEQUENCE [LARGE SCALE MRNA] OF 1-165 (ISOFORM OO).
RC TISSUE=Gastrula {ECO:0000312|EMBL:AAH80883.1}, and
RC Neurula {ECO:0000312|EMBL:CAJ83090.1};
RG Sanger Xenopus tropicalis EST/cDNA project;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000305, ECO:0000312|EMBL:CAJ83090.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM EM).
RC TISSUE=Gastrula {ECO:0000312|EMBL:AAH80883.1};
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000305}
RP IDENTIFICATION, AND ALTERNATIVE SPLICING.
RX PubMed=12351198; DOI=10.1016/s0925-4773(02)00281-2;
RA Schohl A., Barreto G., Joos T.O., Dreyer C.;
RT "Oocytes and embryos of Xenopus laevis express two different isoforms of
RT germ cell nuclear factor (GCNF, NR6A1).";
RL Mech. Dev. 118:261-264(2002).
CC -!- FUNCTION: Probable orphan nuclear receptor. Binds to a response element
CC containing repeats of the motif 5'-AGGTCA-3'. Required for anterior-
CC posterior patterning during organogenesis. Acts with chordin to play a
CC role in patterning the midbrain-hindbrain. Isoform Em is required for
CC integrin-mediated cell matrix interaction during neurulation and for
CC the morphogenetic movements leading to formation of the neural tube.
CC Also mediates the effect of retinoic acid on primary neurogenesis (By
CC similarity). {ECO:0000250|UniProtKB:P70033}.
CC -!- SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:P70033}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:P70033}. Nucleus
CC {ECO:0000250|UniProtKB:P70033, ECO:0000255|PROSITE-ProRule:PRU00407}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=Em; Synonyms=xtEm {ECO:0000269|PubMed:12351198};
CC IsoId=Q66JK1-1; Sequence=Displayed;
CC Name=Oo; Synonyms=xtOo {ECO:0000269|PubMed:12351198};
CC IsoId=Q66JK1-2; Sequence=VSP_052453;
CC -!- SIMILARITY: Belongs to the nuclear hormone receptor family. NR6
CC subfamily. {ECO:0000255}.
CC -!- SEQUENCE CAUTION: [Isoform Oo]:
CC Sequence=AL630347; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AL630347; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; CR760283; CAJ83090.1; -; mRNA.
DR EMBL; BC080883; AAH80883.1; -; mRNA.
DR RefSeq; NP_001008005.1; NM_001008004.1. [Q66JK1-1]
DR RefSeq; XP_012823721.1; XM_012968267.1. [Q66JK1-2]
DR AlphaFoldDB; Q66JK1; -.
DR SMR; Q66JK1; -.
DR STRING; 8364.ENSXETP00000018759; -.
DR PaxDb; Q66JK1; -.
DR DNASU; 493367; -.
DR GeneID; 493367; -.
DR KEGG; xtr:493367; -.
DR CTD; 2649; -.
DR Xenbase; XB-GENE-487592; nr6a1.
DR eggNOG; KOG3575; Eukaryota.
DR HOGENOM; CLU_007368_6_1_1; -.
DR InParanoid; Q66JK1; -.
DR OMA; SPEAYVC; -.
DR OrthoDB; 619653at2759; -.
DR TreeFam; TF350737; -.
DR Reactome; R-XTR-383280; Nuclear Receptor transcription pathway.
DR Proteomes; UP000008143; Chromosome 8.
DR Proteomes; UP000790000; Unplaced.
DR Bgee; ENSXETG00000008578; Expressed in neurula embryo and 7 other tissues.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0004879; F:nuclear receptor activity; IBA:GO_Central.
DR GO; GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; ISS:UniProtKB.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0048513; P:animal organ development; ISS:UniProtKB.
DR GO; GO:0009952; P:anterior/posterior pattern specification; ISS:UniProtKB.
DR GO; GO:0016477; P:cell migration; ISS:UniProtKB.
DR GO; GO:0030917; P:midbrain-hindbrain boundary development; ISS:UniProtKB.
DR GO; GO:0022008; P:neurogenesis; ISS:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 1.10.565.10; -; 1.
DR Gene3D; 3.30.50.10; -; 1.
DR InterPro; IPR035500; NHR-like_dom_sf.
DR InterPro; IPR000536; Nucl_hrmn_rcpt_lig-bd.
DR InterPro; IPR001723; Nuclear_hrmn_rcpt.
DR InterPro; IPR001628; Znf_hrmn_rcpt.
DR InterPro; IPR013088; Znf_NHR/GATA.
DR Pfam; PF00104; Hormone_recep; 1.
DR Pfam; PF00105; zf-C4; 1.
DR PRINTS; PR00398; STRDHORMONER.
DR PRINTS; PR00047; STROIDFINGER.
DR SMART; SM00430; HOLI; 1.
DR SMART; SM00399; ZnF_C4; 1.
DR SUPFAM; SSF48508; SSF48508; 1.
DR PROSITE; PS51843; NR_LBD; 1.
DR PROSITE; PS00031; NUCLEAR_REC_DBD_1; 1.
DR PROSITE; PS51030; NUCLEAR_REC_DBD_2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cytoplasm; Developmental protein; Differentiation;
KW DNA-binding; Metal-binding; Neurogenesis; Nucleus; Receptor;
KW Reference proteome; Transcription; Transcription regulation; Zinc;
KW Zinc-finger.
FT CHAIN 1..435
FT /note="Nuclear receptor subfamily 6 group A member 1"
FT /id="PRO_0000289152"
FT DOMAIN 204..435
FT /note="NR LBD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01189"
FT DNA_BIND 11..86
FT /note="Nuclear receptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00407"
FT ZN_FING 14..34
FT /note="NR C4-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00407"
FT ZN_FING 50..69
FT /note="NR C4-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00407"
FT REGION 84..157
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 116..157
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..5
FT /note="MDTWE -> MEVEKGASGLMDSPGFYKEPAGENEHTAGNMSDLSSDGFKMNS
FT LV (in isoform Oo)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_052453"
SQ SEQUENCE 435 AA; 50010 MW; C52F3D2D811CC215 CRC64;
MDTWEDDRVD QRACLICGDR ATGLHYGIIS CEGCKGFFKR SICNKRVYRC SRDKNCVMSR
KQRNRCQYCR LLKCLQMGMN RKAIREDGMP GGRNKSIGPV QISDEEIERI MSGQEFEEEA
NTSWSNNGDS DHSSPGNGVS ESNQPSPVST PSSSRSMELN GFGSLRDQYL GTPGPMHYQY
LPHLFSYSAH PTLIPTQSRS LDPQSHTLIN QLLTAEDIEP LSTPMLIEDG YKVTQSELFA
LLCRLADELL FRQITWVKKL PFFCDLSIKD YTCLLSTTWQ ELILLSSLTT YSKQIFGDLA
DVTSKYSPSE DELHRFSEDG MEVMERLIYL FRKFSQLKVS NEEYVCMKAI NFLNQDIQGI
SSVSQVEQLN KRYWYVCQDF TEYRYPHQPN RFPDLMMCLP EVRYIAGKLV NVPLEQLPLL
FKAFLHSCKT SLTKE
