NRA1_CAEBR
ID NRA1_CAEBR Reviewed; 629 AA.
AC A8WMY4;
DT 15-JUN-2010, integrated into UniProtKB/Swiss-Prot.
DT 16-DEC-2008, sequence version 2.
DT 03-AUG-2022, entry version 70.
DE RecName: Full=Nicotinic receptor-associated protein 1;
GN Name=nra-1 {ECO:0000312|EMBL:CAP21839.2}; ORFNames=CBG00380;
OS Caenorhabditis briggsae.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6238;
RN [1] {ECO:0000312|EMBL:CAP21839.2}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AF16;
RX PubMed=14624247; DOI=10.1371/journal.pbio.0000045;
RA Stein L.D., Bao Z., Blasiar D., Blumenthal T., Brent M.R., Chen N.,
RA Chinwalla A., Clarke L., Clee C., Coghlan A., Coulson A., D'Eustachio P.,
RA Fitch D.H.A., Fulton L.A., Fulton R.E., Griffiths-Jones S., Harris T.W.,
RA Hillier L.W., Kamath R., Kuwabara P.E., Mardis E.R., Marra M.A.,
RA Miner T.L., Minx P., Mullikin J.C., Plumb R.W., Rogers J., Schein J.E.,
RA Sohrmann M., Spieth J., Stajich J.E., Wei C., Willey D., Wilson R.K.,
RA Durbin R.M., Waterston R.H.;
RT "The genome sequence of Caenorhabditis briggsae: a platform for comparative
RT genomics.";
RL PLoS Biol. 1:166-192(2003).
CC -!- FUNCTION: Exhibits calcium-dependent phospholipid binding properties.
CC May function in membrane trafficking. Regulates synaptic levels of
CC nicotinic acetylcholine receptor subunit lev-1 and unc-38 in the nerve
CC cord. Involved in nicotinic acetylcholine receptor (nAChR)-mediated
CC sensitivity to nicotine and levamisole. Affects directional sperm
CC motility. {ECO:0000250|UniProtKB:Q99829, ECO:0000250|UniProtKB:Q9XUB9}.
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00041};
CC -!- SUBUNIT: Interacts with nicotinic acetylcholine receptor.
CC {ECO:0000250|UniProtKB:Q9XUB9}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q9XUB9};
CC Peripheral membrane protein {ECO:0000250|UniProtKB:Q9XUB9}.
CC -!- DOMAIN: C2 domains are necessary for calcium-dependent cell membrane
CC association. C2 domains are necessary for neuronal progenitor cell
CC differentiation in a calcium-independent manner.
CC {ECO:0000250|UniProtKB:Q99829}.
CC -!- SIMILARITY: Belongs to the copine family. {ECO:0000255}.
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DR EMBL; HE600968; CAP21839.2; -; Genomic_DNA.
DR AlphaFoldDB; A8WMY4; -.
DR SMR; A8WMY4; -.
DR STRING; 6238.CBG00380; -.
DR EnsemblMetazoa; CBG00380a.1; CBG00380a.1; WBGene00023776.
DR WormBase; CBG00380a; CBP37071; WBGene00023776; Cbr-nra-1.
DR eggNOG; KOG1327; Eukaryota.
DR HOGENOM; CLU_020452_3_2_1; -.
DR InParanoid; A8WMY4; -.
DR OMA; DMHATIR; -.
DR OrthoDB; 1067545at2759; -.
DR Proteomes; UP000008549; Chromosome IV.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005544; F:calcium-dependent phospholipid binding; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0071277; P:cellular response to calcium ion; IBA:GO_Central.
DR GO; GO:0097120; P:receptor localization to synapse; IEA:EnsemblMetazoa.
DR CDD; cd04047; C2B_Copine; 1.
DR CDD; cd01459; vWA_copine_like; 1.
DR Gene3D; 2.60.40.150; -; 2.
DR InterPro; IPR000008; C2_dom.
DR InterPro; IPR035892; C2_domain_sf.
DR InterPro; IPR037768; C2B_Copine.
DR InterPro; IPR045052; Copine.
DR InterPro; IPR010734; Copine_C.
DR InterPro; IPR002035; VWF_A.
DR InterPro; IPR036465; vWFA_dom_sf.
DR PANTHER; PTHR10857; PTHR10857; 1.
DR Pfam; PF00168; C2; 2.
DR Pfam; PF07002; Copine; 1.
DR SMART; SM00239; C2; 2.
DR SMART; SM00327; VWA; 1.
DR SUPFAM; SSF49562; SSF49562; 2.
DR SUPFAM; SSF53300; SSF53300; 1.
DR PROSITE; PS50004; C2; 2.
PE 3: Inferred from homology;
KW Calcium; Cell membrane; Membrane; Metal-binding; Reference proteome;
KW Repeat.
FT CHAIN 1..629
FT /note="Nicotinic receptor-associated protein 1"
FT /id="PRO_0000394770"
FT DOMAIN 1..144
FT /note="C2 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT DOMAIN 162..299
FT /note="C2 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT DOMAIN 342..561
FT /note="VWFA"
FT /evidence="ECO:0000255"
FT REGION 581..600
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 607..629
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 33
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 33
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 39
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 108
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 108
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 110
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 110
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 122
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 192
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 192
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 198
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 254
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 254
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 256
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 256
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 274
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
SQ SEQUENCE 629 AA; 70290 MW; 20CA0A1175C5D8EE CRC64;
MNQPIAAIGI SDSTRPKTNV RLTISAEHLM DTDVFSKSDP ICLVYEKTSG KKATTTEPVQ
VPTWQDSQWT ERGRTEVVMN NLNPQFTKTF LLPYFFEETQ LLRFEIYDAD SPNVGQDLSP
HDFLGRFECV LAQIVSYSTL KAHLGKAEGI GAQWRNKDKN TRTGSITIRA EEDEKCEKIQ
FDVCGEGLDK KDFFGKSDPY LNFKRKFDDG SAHLVHRTEV KQKTLDPRWA TVQINTQTLC
GKDGERPIII ECYDHDKWKK GEEPRGEAKF SRDDHIGTAH TTLNELLRGG AGDPVELLLT
NEKKKAKKGD KYKCSGTLKI WNSKIIVEPT FLDFISGGTQ LEFAVAVDFT ASNGAPKNSS
SLHYMSADRP NQYELALRSV LSICQHYNSS KTFEAFGFGA KLPNSVSVSA IFSLDLLRGT
PEVVGISGVM SAYRHALQNV QLYGPTNFAP IIDTVAQKAQ NMIHDSARYQ ILLIITDGII
SDMHATIRSI INASGLPLSI IIIGVGNEDF EKMHELDSDD SLLQQDSRIA QRDIVQFVTI
REFLNNGRGV YLDPDVVQEN LAREVLFEVP GQLTGYMKQR GFQPRPVENP WTRNSPPPDY
DPVLDGIGRR AQAPSPGFQM PVASAPPMY
