NRA4_CAEEL
ID NRA4_CAEEL Reviewed; 1123 AA.
AC H2KYE0; Q9UAN9;
DT 13-NOV-2019, integrated into UniProtKB/Swiss-Prot.
DT 21-MAR-2012, sequence version 1.
DT 03-AUG-2022, entry version 67.
DE RecName: Full=Nicotinic receptor-associated protein 4 {ECO:0000303|PubMed:19609303};
DE Flags: Precursor;
GN Name=nra-4 {ECO:0000303|PubMed:19609303, ECO:0000312|WormBase:C02E11.1b};
GN ORFNames=C02E11.1 {ECO:0000312|WormBase:C02E11.1b};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION, INTERACTION WITH NRA-2, SUBCELLULAR LOCATION, TISSUE SPECIFICITY,
RP DEVELOPMENTAL STAGE, AND MUTAGENESIS OF 822-SER--ILE-922.
RX PubMed=19609303; DOI=10.1038/emboj.2009.204;
RA Almedom R.B., Liewald J.F., Hernando G., Schultheis C., Rayes D., Pan J.,
RA Schedletzky T., Hutter H., Bouzat C., Gottschalk A.;
RT "An ER-resident membrane protein complex regulates nicotinic acetylcholine
RT receptor subunit composition at the synapse.";
RL EMBO J. 28:2636-2649(2009).
CC -!- FUNCTION: Involved in the recognition and selection of protein
CC complexes to exit the endoplasmic reticulum (ER) (PubMed:19609303). In
CC muscles, regulates levamisole-sensitive nicotinic acetylcholine
CC receptor (L-AChR) subunit composition, possibly by allowing only
CC specific L-AChR subunit combinations to exit the ER (PubMed:19609303).
CC Specifically, may promote the inclusion of alpha subunit unc-38 into
CC and the exclusion of unc-29 from L-AChR (PubMed:19609303). Regulates L-
CC AChR sensitivity to agonists such as nicotine and levamisole at neuro-
CC muscular junctions (PubMed:19609303). {ECO:0000269|PubMed:19609303}.
CC -!- SUBUNIT: May interact with nra-2 in the ER.
CC {ECO:0000269|PubMed:19609303}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:19609303}; Single-pass type I membrane protein
CC {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=b {ECO:0000312|WormBase:C02E11.1b};
CC IsoId=H2KYE0-1; Sequence=Displayed;
CC Name=a {ECO:0000312|WormBase:C02E11.1a};
CC IsoId=H2KYE0-2; Sequence=VSP_060398;
CC -!- TISSUE SPECIFICITY: Expressed in body wall, pharyngeal, uterine and
CC vulval muscles, motor neurons, nerve ring, motor and ventral cord
CC neurons, hypodermal cells in the tail, vulval epithelium and intestine.
CC {ECO:0000269|PubMed:19609303}.
CC -!- DEVELOPMENTAL STAGE: Expressed in embryos and adults.
CC {ECO:0000269|PubMed:19609303}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; BX284605; CCD62613.1; -; Genomic_DNA.
DR EMBL; BX284605; CCD62614.1; -; Genomic_DNA.
DR PIR; T33764; T33764.
DR RefSeq; NP_503367.1; NM_070966.3. [H2KYE0-2]
DR RefSeq; NP_872191.1; NM_182391.1. [H2KYE0-1]
DR AlphaFoldDB; H2KYE0; -.
DR STRING; 6239.C02E11.1b; -.
DR EPD; H2KYE0; -.
DR PaxDb; H2KYE0; -.
DR PeptideAtlas; H2KYE0; -.
DR EnsemblMetazoa; C02E11.1a.1; C02E11.1a.1; WBGene00015344. [H2KYE0-2]
DR EnsemblMetazoa; C02E11.1b.1; C02E11.1b.1; WBGene00015344. [H2KYE0-1]
DR GeneID; 3565008; -.
DR KEGG; cel:CELE_C02E11.1; -.
DR UCSC; C02E11.1a.1; c. elegans.
DR CTD; 3565008; -.
DR WormBase; C02E11.1a; CE28528; WBGene00015344; nra-4. [H2KYE0-2]
DR WormBase; C02E11.1b; CE32040; WBGene00015344; nra-4. [H2KYE0-1]
DR eggNOG; KOG1948; Eukaryota.
DR GeneTree; ENSGT00390000000089; -.
DR HOGENOM; CLU_007543_2_0_1; -.
DR InParanoid; H2KYE0; -.
DR OMA; GWSFEPE; -.
DR OrthoDB; 839381at2759; -.
DR PhylomeDB; H2KYE0; -.
DR PRO; PR:H2KYE0; -.
DR Proteomes; UP000001940; Chromosome V.
DR Bgee; WBGene00015344; Expressed in pharyngeal muscle cell (C elegans) and 4 other tissues.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:WormBase.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0065003; P:protein-containing complex assembly; IMP:UniProtKB.
PE 1: Evidence at protein level;
KW Alternative splicing; Endoplasmic reticulum; Membrane; Reference proteome;
KW Signal; Transmembrane; Transmembrane helix.
FT SIGNAL 1..18
FT /evidence="ECO:0000255"
FT CHAIN 19..1123
FT /note="Nicotinic receptor-associated protein 4"
FT /evidence="ECO:0000255"
FT /id="PRO_5003564292"
FT TOPO_DOM 19..1071
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT TRANSMEM 1072..1092
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1093..1123
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT VAR_SEQ 40..41
FT /note="Missing (in isoform a)"
FT /evidence="ECO:0000305"
FT /id="VSP_060398"
FT MUTAGEN 822..922
FT /note="Missing: In tm2656; no resistance to levamisole or
FT nicotine-induced paralysis. No defect in levamisole or
FT nicotine-induced postsynaptic currents in muscles."
FT /evidence="ECO:0000269|PubMed:19609303"
SQ SEQUENCE 1123 AA; 121901 MW; 4DD9282D8DC83354 CRC64;
MGSLPLILLS LLLPGALANV YSCAGSVKST SPVDYSQLKL QVRLLTLEGH MKHEEEVNPS
NGYFMIPVYN KGHYTLKVSA PAGYYFEPDS IEIKIDGKTD ACSLNEDLVF HLTGFSVRGT
VDGAAAGLPL VLTENGKQIA ETKTEDGGKY EMRAPPGKYE VSTGAGASEC ISKGKTSVEV
KNAPVVVTPN FKISGYQLEV HTRTESMNPF VDAVMTLYAT SSIDLPNIKC VGSEGSLNVP
STHNVKCSIG KTDPRGRLSV ACVPSGEYYL AASHVNGPKS INFSPNPQKV VVSQAASEAR
FVAQSATGRV RVTSKDLPLS GVEVLVNEKS GGKTDSQGYL KIENLKEDEH TTITAKAPNT
QFSTVHANVK FPKVEIQDVT VQKFDICGQV EKSENGVLGK LTFTRKDDKR SLEIQPKADG
SFCQPVSPGL FTIEPTDKTS SLTPRLLEVE VLKNAVTNLR FTHFKTNANV HLSCIGACPT
ATVSLFLPGQ TLVRSVKGTD VFTFENIGPG TYSARLDDNG RGCWEKSEMT LVVEQSNTQP
TIHFKQNGFA AQIEISHPAE IEWSNADKKQ LNGKTSTKGG EVISICVPTS GVYDVSLGSC
YKFERQQFKL TVPFDGVHKE KAVAARISGT IDLENDKNAA VSIRIKSSAG DREIQVPALD
NGRFTFEEPL ASSGEQLVIV PSSKLRLFEP TSKSVTVTGK CIENAVKFNS FRGIFLDGSI
KPAVEKAVVK AVLKKDKDVV IEAISNKDGA FKIGPVKRVE DYDITATLDG FKFTPTSTPG
HFQSVKLSQL SIKVVDEVTN APLDGVLLSL VGGKGAGSDY RSNNVLDETA HKNYVALAPG
EYFVRAILQE YKFSPSTSTI VVKEGQHENV VLKGKRVSFS AYGKMREMSG DAMKDVIIEA
LSEGCDLHQS EATTKEDGTY RIRGLLPDCE YQVHAKSYAD GSPAPHSFPR SFTVSMTAED
VKGLEFMATI TAKTTDIAVE IGMDTLPEIQ SVRVVITKNN NDHVQVASVV APQHLHYLVN
LPRDGVEYAI RVEAEKPPQA FAAKTVRVVA DQAMKVARVP LTSSKRANDV DISVGTFLSL
PFFVTLALVF FNQNRVLELL GTFIDWARNT FAPTADNHHR KRK
