NRAM1_ARATH
ID NRAM1_ARATH Reviewed; 532 AA.
AC Q9SAH8; A0JPT0; Q8L7N5; Q8LCS7; Q9SP94;
DT 11-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Metal transporter Nramp1;
DE Short=AtNramp1;
GN Name=NRAMP1; Synonyms=PMIT1; OrderedLocusNames=At1g80830;
GN ORFNames=F23A5.18;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND INDUCTION.
RX PubMed=10769179; DOI=10.1042/bj3470749;
RA Curie C., Alonso J.M., Le Jean M., Ecker J.R., Briat J.-F.;
RT "Involvement of NRAMP1 from Arabidopsis thaliana in iron transport.";
RL Biochem. J. 347:749-755(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Hagai K., Nelson H., Nelson N.;
RT "A metal ion transporter from Arabidopsis thaliana.";
RL Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Bautista V.R., Kim C.J., Chen H., Quinitio C., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 252-532.
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [8]
RP FUNCTION, AND INDUCTION.
RX PubMed=10781110; DOI=10.1073/pnas.97.9.4991;
RA Thomine S., Wang R., Ward J.M., Crawford N.M., Schroeder J.I.;
RT "Cadmium and iron transport by members of a plant metal transporter family
RT in Arabidopsis with homology to Nramp genes.";
RL Proc. Natl. Acad. Sci. U.S.A. 97:4991-4996(2000).
RN [9]
RP FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, INDUCTION,
RP AND DISRUPTION PHENOTYPE.
RX PubMed=20228245; DOI=10.1105/tpc.109.073023;
RA Cailliatte R., Schikora A., Briat J.F., Mari S., Curie C.;
RT "High-affinity manganese uptake by the metal transporter NRAMP1 is
RT essential for Arabidopsis growth in low manganese conditions.";
RL Plant Cell 22:904-917(2010).
CC -!- FUNCTION: High affinity manganese (Mn) transporter involved in Mn
CC acquisition from the soil. Required for Mn uptake into the root in
CC conditions of low Mn availability. Can transport iron (Fe), cadmium
CC (Cd) and cobalt (Co). {ECO:0000269|PubMed:10769179,
CC ECO:0000269|PubMed:10781110, ECO:0000269|PubMed:20228245}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=28 nM for Mn (2+) {ECO:0000269|PubMed:20228245};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:20228245};
CC Multi-pass membrane protein {ECO:0000269|PubMed:20228245}.
CC -!- INDUCTION: By Mn and Fe deficiency in roots.
CC {ECO:0000269|PubMed:10769179, ECO:0000269|PubMed:10781110,
CC ECO:0000269|PubMed:20228245}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC condition, but under Mn limitation, very slow growth and unable to take
CC up Mn. {ECO:0000269|PubMed:20228245}.
CC -!- SIMILARITY: Belongs to the NRAMP (TC 2.A.55) family. {ECO:0000305}.
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DR EMBL; AF165125; AAF36535.1; -; mRNA.
DR EMBL; AF181687; AAD54417.1; -; mRNA.
DR EMBL; AC011713; AAF14670.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE36455.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM59231.1; -; Genomic_DNA.
DR EMBL; BT029300; ABK32114.1; -; mRNA.
DR EMBL; AY086428; AAM63430.1; -; mRNA.
DR EMBL; AY128347; AAM91550.1; -; mRNA.
DR PIR; H96840; H96840.
DR RefSeq; NP_001321606.1; NM_001335016.1.
DR RefSeq; NP_178198.1; NM_106731.3.
DR AlphaFoldDB; Q9SAH8; -.
DR SMR; Q9SAH8; -.
DR BioGRID; 29640; 4.
DR IntAct; Q9SAH8; 4.
DR STRING; 3702.AT1G80830.1; -.
DR TCDB; 2.A.55.2.4; the metal ion (mn(2+)-iron) transporter (nramp) family.
DR PaxDb; Q9SAH8; -.
DR PRIDE; Q9SAH8; -.
DR ProteomicsDB; 250847; -.
DR EnsemblPlants; AT1G80830.1; AT1G80830.1; AT1G80830.
DR EnsemblPlants; AT1G80830.2; AT1G80830.2; AT1G80830.
DR GeneID; 844422; -.
DR Gramene; AT1G80830.1; AT1G80830.1; AT1G80830.
DR Gramene; AT1G80830.2; AT1G80830.2; AT1G80830.
DR KEGG; ath:AT1G80830; -.
DR Araport; AT1G80830; -.
DR TAIR; locus:2025677; AT1G80830.
DR eggNOG; KOG1291; Eukaryota.
DR HOGENOM; CLU_020088_0_1_1; -.
DR InParanoid; Q9SAH8; -.
DR OMA; FIKLLIH; -.
DR OrthoDB; 666470at2759; -.
DR PhylomeDB; Q9SAH8; -.
DR PRO; PR:Q9SAH8; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9SAH8; baseline and differential.
DR Genevisible; Q9SAH8; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0015086; F:cadmium ion transmembrane transporter activity; IDA:UniProtKB.
DR GO; GO:0005381; F:iron ion transmembrane transporter activity; IGI:TAIR.
DR GO; GO:0005384; F:manganese ion transmembrane transporter activity; IDA:UniProtKB.
DR GO; GO:0070574; P:cadmium ion transmembrane transport; IDA:UniProtKB.
DR GO; GO:0055072; P:iron ion homeostasis; IEA:UniProtKB-KW.
DR GO; GO:0034755; P:iron ion transmembrane transport; IGI:TAIR.
DR GO; GO:0055071; P:manganese ion homeostasis; IMP:TAIR.
DR GO; GO:0071421; P:manganese ion transmembrane transport; IDA:UniProtKB.
DR HAMAP; MF_00221; NRAMP; 1.
DR InterPro; IPR001046; NRAMP_fam.
DR PANTHER; PTHR11706; PTHR11706; 1.
DR Pfam; PF01566; Nramp; 1.
DR PRINTS; PR00447; NATRESASSCMP.
DR TIGRFAMs; TIGR01197; nramp; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Ion transport; Iron; Iron transport; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..532
FT /note="Metal transporter Nramp1"
FT /id="PRO_0000212598"
FT TRANSMEM 46..66
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 79..99
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 123..143
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 151..171
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 181..201
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 229..249
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 266..286
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 329..349
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 372..392
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 393..413
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 431..451
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 466..486
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..25
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 200
FT /note="L -> I (in Ref. 2; AAD54417)"
FT /evidence="ECO:0000305"
FT CONFLICT 390
FT /note="A -> S (in Ref. 7; AAM91550)"
FT /evidence="ECO:0000305"
FT CONFLICT 434
FT /note="L -> F (in Ref. 2; AAD54417)"
FT /evidence="ECO:0000305"
FT CONFLICT 532
FT /note="D -> G (in Ref. 6; AAM63430)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 532 AA; 57562 MW; 57AF076FDE267247 CRC64;
MAATGSGRSQ FISSSGGNRS FSNSPLIENS DSNQIIVSEK KSWKNFFAYL GPGFLVSIAY
IDPGNFETDL QAGAHYKYEL LWIILVASCA ALVIQSLAAN LGVVTGKHLA EQCRAEYSKV
PNFMLWVVAE IAVVACDIPE VIGTAFALNM LFSIPVWIGV LLTGLSTLIL LALQKYGVRK
LEFLIAFLVF TIAICFFVEL HYSKPDPGEV LHGLFVPQLK GNGATGLAIS LLGAMVMPHN
LFLHSALVLS RKIPRSASGI KEACRFYLIE SGLALMVAFL INVSVISVSG AVCNAPNLSP
EDRANCEDLD LNKASFLLRN VVGKWSSKLF AIALLASGQS STITGTYAGQ YVMQGFLDLR
LEPWLRNLLT RCLAIIPSLI VALIGGSAGA GKLIIIASMI LSFELPFALV PLLKFTSCKT
KMGSHVNPMA ITALTWVIGG LIMGINIYYL VSSFIKLLIH SHMKLILVVF CGILGFAGIA
LYLAAIAYLV FRKNRVATSL LISRDSQNVE TLPRQDIVNM QLPCRVSTSD VD
