NRAM1_CANLF
ID NRAM1_CANLF Reviewed; 547 AA.
AC Q9XT74; Q0PPT4;
DT 21-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 2.
DT 25-MAY-2022, entry version 103.
DE RecName: Full=Natural resistance-associated macrophage protein 1;
DE Short=NRAMP 1;
DE AltName: Full=Solute carrier family 11 member 1;
GN Name=SLC11A1; Synonyms=NRAMP1;
OS Canis lupus familiaris (Dog) (Canis familiaris).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Carnivora; Caniformia; Canidae; Canis.
OX NCBI_TaxID=9615 {ECO:0000312|EMBL:AAD37483.2};
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS LEU-58; SER-180; ASN-323;
RP ALA-413 AND THR-476.
RX PubMed=12010961; DOI=10.1128/iai.70.6.2763-2771.2002;
RA Altet L., Francino O., Solano-Gallego L., Renier C., Sanchez A.;
RT "Mapping and sequencing of the canine NRAMP1 gene and identification of
RT mutations in leishmaniasis-susceptible dogs.";
RL Infect. Immun. 70:2763-2771(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], AND VARIANT ALA-413.
RA Bueno R., Santos D.N., Melo M.N., Santos R.L.;
RT "Canine Nramp1 and visceral leishmaniasis.";
RL Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Divalent transition metal (iron and manganese) transporter
CC involved in iron metabolism and host resistance to certain pathogens.
CC Macrophage-specific membrane transport function. Controls natural
CC resistance to infection with intracellular parasites. Pathogen
CC resistance involves sequestration of Fe(2+) and Mn(2+), cofactors of
CC both prokaryotic and eukaryotic catalases and superoxide dismutases,
CC not only to protect the macrophage against its own generation of
CC reactive oxygen species, but to deny the cations to the pathogen for
CC synthesis of its protective enzymes (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the NRAMP family.
CC {ECO:0000250|UniProtKB:P49279}.
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DR EMBL; AF091049; AAD37483.2; -; Genomic_DNA.
DR EMBL; DQ784645; ABG78262.1; -; mRNA.
DR RefSeq; NP_001013873.1; NM_001013851.1.
DR AlphaFoldDB; Q9XT74; -.
DR SMR; Q9XT74; -.
DR STRING; 9615.ENSCAFP00000021599; -.
DR PaxDb; Q9XT74; -.
DR GeneID; 478909; -.
DR KEGG; cfa:478909; -.
DR CTD; 6556; -.
DR eggNOG; KOG1291; Eukaryota.
DR InParanoid; Q9XT74; -.
DR OrthoDB; 666470at2759; -.
DR Proteomes; UP000002254; Unplaced.
DR GO; GO:0016021; C:integral component of membrane; NAS:UniProtKB.
DR GO; GO:0005384; F:manganese ion transmembrane transporter activity; NAS:UniProtKB.
DR GO; GO:0055072; P:iron ion homeostasis; IEA:UniProtKB-KW.
DR GO; GO:0006828; P:manganese ion transport; NAS:UniProtKB.
DR HAMAP; MF_00221; NRAMP; 1.
DR InterPro; IPR001046; NRAMP_fam.
DR PANTHER; PTHR11706; PTHR11706; 1.
DR Pfam; PF01566; Nramp; 1.
DR PRINTS; PR00447; NATRESASSCMP.
DR TIGRFAMs; TIGR01197; nramp; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Ion transport; Iron; Iron transport; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..547
FT /note="Natural resistance-associated macrophage protein 1"
FT /id="PRO_0000212586"
FT TOPO_DOM 1..54
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 55..75
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 76..81
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 82..102
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 103..139
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 140..160
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 161..164
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 165..185
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 186..193
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 194..214
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 215..240
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 241..261
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 262..286
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 287..307
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 308..346
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 347..367
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 368..394
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 395..415
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 416..432
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 433..453
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 454..464
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 465..485
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 486..492
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 493..513
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 514..547
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 1..30
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 321
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 335
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VARIANT 58
FT /note="F -> L"
FT /evidence="ECO:0000269|PubMed:12010961"
FT VARIANT 180
FT /note="F -> S"
FT /evidence="ECO:0000269|PubMed:12010961"
FT VARIANT 323
FT /note="S -> N"
FT /evidence="ECO:0000269|PubMed:12010961"
FT VARIANT 413
FT /note="V -> A"
FT /evidence="ECO:0000269|PubMed:12010961, ECO:0000269|Ref.2"
FT VARIANT 476
FT /note="I -> T"
FT /evidence="ECO:0000269|PubMed:12010961"
SQ SEQUENCE 547 AA; 60121 MW; E438FABC2175FD10 CRC64;
MTGDKDPQSV SRPNYGSISH PPSSEPQQEP LRTTYLSEKI PIPDTEPGTF SLRKLWAFTG
PGFLMSIAFL DPGNIESDLQ AGAVAGFKLL WVLLWATVLG LLCQRLAARL GVVTGKDLGE
ICHLYYPKVP RTLLWLTIEL AIVGSDMQEV IGTAIAFSLL SAGRIPLWGG VLITIVDTFF
FLFLDNYGLR KLEAFFGILI TIMALTFGYE YVVARPAQVA LLQGLLLPSC PGCGRPELLQ
AVGIVGAIIM PHNIYLHSAL VKSREIDRSR RPDIREANMY FLIEASIALS VSFFINLFVV
AVFGQAFYQQ TNEAAFNVCA NSSLHDYAKI FPRNNLTVEV DIYQGGVMLG CVFGPAALYI
WAVGLLAAGQ SSTMTGTYAG QFVMEGFLRL RWSRFARVLL TRSCAILPTV LVVVFRDLKD
LSGLNDLLNV LQSLLLPFAV LPILTFTSMP ALMQEFANGR LSKAITSFIM ALVCAINLYF
VVIYLPSLPH PAYFILVALL AIVYLGLTTY LVWTCFIAHG VTLLAHSSHQ HFLYGLPDVE
EKGKISG
