NRAM1_DICDI
ID NRAM1_DICDI Reviewed; 533 AA.
AC Q869V1; Q550F8;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 25-MAY-2022, entry version 103.
DE RecName: Full=Metal transporter nramp1 homolog;
DE AltName: Full=Natural resistance-associated membrane protein 1;
GN Name=nramp1; Synonyms=nramp, slc11a1; ORFNames=DDB_G0276973;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=12097910; DOI=10.1038/nature00847;
RA Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA Noegel A.A.;
RT "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL Nature 418:79-85(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE, AND SUBCELLULAR
RP LOCATION.
RX PubMed=16445684; DOI=10.1111/j.1600-0854.2005.00356.x;
RA Peracino B., Wagner C., Balest A., Balbo A., Pergolizzi B., Noegel A.A.,
RA Steinert M., Bozzaro S.;
RT "Function and mechanism of action of Dictyostelium Nramp1 (Slc11a1) in
RT bacterial infection.";
RL Traffic 7:22-38(2006).
CC -!- FUNCTION: Depletes iron from the phagolysosome in an ATP-dependent
CC process. May rather act as a symporter of protons and metal cations in
CC an ATP-dependent process. Nramp1 overexpression protected cells from
CC L.pneumophila infection. {ECO:0000269|PubMed:16445684}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}. Note=Located at vesicles of variable size, which
CC are distributed all over the cytoplasm, in part clustered at a
CC perinuclear site. Several tiny vesicles travel rapidly outward from and
CC towards the perinuclear cluster. These vesicles are part of the
CC endo- and phagolysosomal pathway. {ECO:0000269|PubMed:16445684}.
CC -!- DEVELOPMENTAL STAGE: Expressed during the growth-phase and down-
CC regulated to barely detectable levels upon starvation.
CC {ECO:0000269|PubMed:16445684}.
CC -!- DISRUPTION PHENOTYPE: More permissive hosts than wild-type cells for
CC intracellular growth of L.pneumophila and M.avium.
CC {ECO:0000269|PubMed:16445684}.
CC -!- SIMILARITY: Belongs to the NRAMP family. {ECO:0000305}.
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DR EMBL; AAFI02000019; EAL68988.1; -; Genomic_DNA.
DR RefSeq; XP_642974.1; XM_637882.1.
DR AlphaFoldDB; Q869V1; -.
DR SMR; Q869V1; -.
DR STRING; 44689.DDB0232113; -.
DR TCDB; 2.A.55.2.9; the metal ion (mn(2+)-iron) transporter (nramp) family.
DR PaxDb; Q869V1; -.
DR EnsemblProtists; EAL68988; EAL68988; DDB_G0276973.
DR GeneID; 8620846; -.
DR KEGG; ddi:DDB_G0276973; -.
DR dictyBase; DDB_G0276973; nramp1.
DR eggNOG; KOG1291; Eukaryota.
DR HOGENOM; CLU_020088_5_1_1; -.
DR InParanoid; Q869V1; -.
DR OMA; IATFVNS; -.
DR PhylomeDB; Q869V1; -.
DR Reactome; R-DDI-1222556; ROS and RNS production in phagocytes.
DR Reactome; R-DDI-425410; Metal ion SLC transporters.
DR Reactome; R-DDI-6798695; Neutrophil degranulation.
DR Reactome; R-DDI-6803544; Ion influx/efflux at host-pathogen interface.
DR Reactome; R-DDI-917937; Iron uptake and transport.
DR PRO; PR:Q869V1; -.
DR Proteomes; UP000002195; Chromosome 2.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0044354; C:macropinosome; IDA:dictyBase.
DR GO; GO:0016020; C:membrane; IDA:dictyBase.
DR GO; GO:0032010; C:phagolysosome; IDA:dictyBase.
DR GO; GO:0005802; C:trans-Golgi network; IDA:dictyBase.
DR GO; GO:0012506; C:vesicle membrane; IDA:dictyBase.
DR GO; GO:0015086; F:cadmium ion transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015093; F:ferrous iron transmembrane transporter activity; IDA:dictyBase.
DR GO; GO:0005381; F:iron ion transmembrane transporter activity; IDA:dictyBase.
DR GO; GO:0005384; F:manganese ion transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0006879; P:cellular iron ion homeostasis; IDA:dictyBase.
DR GO; GO:0042742; P:defense response to bacterium; IMP:dictyBase.
DR GO; GO:1903988; P:iron ion export across plasma membrane; IMP:dictyBase.
DR GO; GO:0034755; P:iron ion transmembrane transport; IDA:dictyBase.
DR GO; GO:0006828; P:manganese ion transport; IDA:dictyBase.
DR GO; GO:0006909; P:phagocytosis; IEP:dictyBase.
DR GO; GO:0009617; P:response to bacterium; IMP:dictyBase.
DR HAMAP; MF_00221; NRAMP; 1.
DR InterPro; IPR001046; NRAMP_fam.
DR PANTHER; PTHR11706; PTHR11706; 1.
DR Pfam; PF01566; Nramp; 1.
DR PRINTS; PR00447; NATRESASSCMP.
DR TIGRFAMs; TIGR01197; nramp; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Ion transport; Iron; Iron transport; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..533
FT /note="Metal transporter nramp1 homolog"
FT /id="PRO_0000328222"
FT TOPO_DOM 1..68
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 69..89
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 90..101
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 102..122
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 123..158
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 159..179
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 180..182
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 183..203
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 204..212
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 213..233
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 234..256
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 257..277
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 278..302
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 303..323
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 324..348
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 349..368
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 369..387
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 388..408
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 409..415
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 416..436
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 437..457
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 458..478
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 479..481
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 482..502
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 503..533
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 1..33
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 11..33
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 253
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 533 AA; 60034 MW; 0EA56D60A9B4E264 CRC64;
MTPRIESEES APLVNKNNNN NNDNNNNNNV DEENPLIIES GIPIEDLEQK NKPYLIKVPN
IDKPDSKWIN FKTLWAFTGP GFLMSIAYLD PGNLESDIQA GAMAGYQLLW VLFWSTVIGF
WLQMLASRLG VVTGKHLAEH CREQYPKTPR LLLWLMTELA IIGSDIQEVI GTAIALQILS
NGHIPLWAGV LFTAADTFTF LFLEKYGIRK LEAFFCSLIA IMAISFGVEY IISKPDQIEV
VKGVFIPLCS QNNISQAVGI LGAVVMPHNI YLHSALVQSR EIDRKSETQV KIANKYNRLE
SAFALIISFI INLLLVSVFA KGFYGETTEI GLSSAADFLM DKYGKVAKYI WAIGLFSAGQ
CSTMTGTYSG QFVMEGFLKL KIAPWKRLLI TRCTAIVPAM VVAILSTSHL DSLDQWLNIL
QSIQLPFAVV PVLLFTSSEK IMGSKFKNHW LNNQFVRFLS LLIIAINIYL IITFSMQISE
SAWMISIVSI SFFFYFIFIV YLSMGQENFN SMTKKIKNLF NNNSNQTYNN INY
