NRAM1_PIG
ID NRAM1_PIG Reviewed; 538 AA.
AC O77741; Q9N1Y2;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 25-MAY-2022, entry version 100.
DE RecName: Full=Natural resistance-associated macrophage protein 1;
DE Short=NRAMP 1;
DE AltName: Full=Solute carrier family 11 member 1;
GN Name=SLC11A1; Synonyms=NRAMP1;
OS Sus scrofa (Pig).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX NCBI_TaxID=9823;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Spleen;
RX PubMed=9027577; DOI=10.2527/1997.751277x;
RA Tuggle C.K., Schmitz C.B., Gingerich-Feil D.;
RT "Rapid communication: cloning of a pig full-length natural resistance
RT associated macrophage protein (NRAMP1) cDNA.";
RL J. Anim. Sci. 75:277-277(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=10678911; DOI=10.1128/iai.68.3.1086-1093.2000;
RA Zhang G., Wu H., Ross C.R., Minton E., Blecha F.;
RT "Cloning of porcine NRAMP1 and its induction by lipopolysaccharide, tumor
RT necrosis factor alpha, and interleukin-1beta: role of CD14 and mitogen-
RT activated protein kinases.";
RL Infect. Immun. 68:1086-1093(2000).
CC -!- FUNCTION: Divalent transition metal (iron and manganese) transporter
CC involved in iron metabolism and host resistance to certain pathogens.
CC Macrophage-specific membrane transport function. Controls natural
CC resistance to infection with intracellular parasites. Pathogen
CC resistance involves sequestration of Fe(2+) and Mn(2+), cofactors of
CC both prokaryotic and eukaryotic catalases and superoxide dismutases,
CC not only to protect the macrophage against its own generation of
CC reactive oxygen species, but to deny the cations to the pathogen for
CC synthesis of its protective enzymes (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the NRAMP family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U55068; AAC24491.1; -; mRNA.
DR EMBL; AF132037; AAF36527.1; -; mRNA.
DR RefSeq; NP_998986.1; NM_213821.2.
DR AlphaFoldDB; O77741; -.
DR SMR; O77741; -.
DR STRING; 9823.ENSSSCP00000020374; -.
DR PaxDb; O77741; -.
DR GeneID; 396764; -.
DR KEGG; ssc:396764; -.
DR CTD; 6556; -.
DR eggNOG; KOG1291; Eukaryota.
DR InParanoid; O77741; -.
DR OrthoDB; 666470at2759; -.
DR Proteomes; UP000008227; Unplaced.
DR Proteomes; UP000314985; Unplaced.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0046873; F:metal ion transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0055072; P:iron ion homeostasis; IEA:UniProtKB-KW.
DR GO; GO:0000165; P:MAPK cascade; IDA:MGI.
DR GO; GO:0032496; P:response to lipopolysaccharide; IDA:MGI.
DR HAMAP; MF_00221; NRAMP; 1.
DR InterPro; IPR001046; NRAMP_fam.
DR PANTHER; PTHR11706; PTHR11706; 1.
DR Pfam; PF01566; Nramp; 1.
DR PRINTS; PR00447; NATRESASSCMP.
DR TIGRFAMs; TIGR01197; nramp; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Ion transport; Iron; Iron transport; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..538
FT /note="Natural resistance-associated macrophage protein 1"
FT /id="PRO_0000212590"
FT TOPO_DOM 1..54
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 55..75
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 76..81
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 82..102
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 103..139
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 140..160
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 161..164
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 165..185
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 186..193
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 194..214
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 215..240
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 241..261
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 262..286
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 287..307
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 308..346
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 347..367
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 368..394
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 395..415
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 416..432
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 433..453
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 454..468
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 469..489
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 490..492
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 493..513
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 514..538
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 1..36
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..22
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 321
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 335
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 2
FT /note="T -> S (in Ref. 2; AAF36527)"
FT /evidence="ECO:0000305"
FT CONFLICT 6
FT /note="D -> G (in Ref. 2; AAF36527)"
FT /evidence="ECO:0000305"
FT CONFLICT 137
FT /note="N -> T (in Ref. 2; AAF36527)"
FT /evidence="ECO:0000305"
FT CONFLICT 164
FT /note="R -> G (in Ref. 2; AAF36527)"
FT /evidence="ECO:0000305"
FT CONFLICT 185
FT /note="N -> D (in Ref. 2; AAF36527)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 538 AA; 59012 MW; 235DE941CDEB2E5B CRC64;
MTGDTDPPKQ SRTQYGSISS SPSPGPPQVP PGGTYLSEKI PIPNAEPGTF SLRKLWAFTG
PGFLMSIAYL DPGNIQSDLQ AGAVAGFKLL WVLLWATVLG LLCQRLAARL GVVTGKDLGE
ICHLYYPKVP RTLLWLNMEL AIVGSDMQEV IGTAIAFNLL SAGRIPLWGG VLITVVDTFF
FLYLNNYGLR KLEAFFAFLI AIMAFTFGYE YVVARPAQGA LLRGLFLPSC SGCGQPELLQ
AVGIVGAIIM PHNIYLHSAL VKSREVDRTR REDIREANMY FLIESTIALF VSFFINLFVM
AVFGQAFYQQ TNQAAFNICA NSSLHDYAKI FPRNNLTVAV DFYQGGVILG CLFGPAALYI
WAVGLLAAGQ SSTMTGTYAG QFVMEGFLKL RWSRFARLLL TRSCAILPAL LVAVFKELQD
LSSLNDLLNV LQSLLLPFAV LPILTFTSMP ALMQEFASGR VNKVITSSIM LLVCAINFYF
LVSYLPSLPH PAYFGLVALL AVIYLGLTTY LVWTCLIAHG ATLLVHSSHQ HFLYGLLE
