NRAM1_RAT
ID NRAM1_RAT Reviewed; 507 AA.
AC P70553; Q496Z6;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 10-JAN-2006, sequence version 3.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Natural resistance-associated macrophage protein 1;
DE Short=NRAMP 1;
DE AltName: Full=Solute carrier family 11 member 1;
GN Name=Slc11a1; Synonyms=Bcg, Nramp1;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Prostate;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 336-507.
RC STRAIN=Lewis;
RX PubMed=8875897; DOI=10.1007/s003359900251;
RA Ge L., Remmers E.F., Du Y., Wilder R.L.;
RT "Genomic cloning and genetic mapping of the rat Nramp1 (Bcg) gene on
RT chromosome 9.";
RL Mamm. Genome 7:856-857(1996).
CC -!- FUNCTION: Divalent transition metal (iron and manganese) transporter
CC involved in iron metabolism and host resistance to certain pathogens.
CC Macrophage-specific membrane transport function. Controls natural
CC resistance to infection with intracellular parasites. Pathogen
CC resistance involves sequestration of Fe(2+) and Mn(2+), cofactors of
CC both prokaryotic and eukaryotic catalases and superoxide dismutases,
CC not only to protect the macrophage against its own generation of
CC reactive oxygen species, but to deny the cations to the pathogen for
CC synthesis of its protective enzymes (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the NRAMP family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB49833.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; BC100653; AAI00654.1; -; mRNA.
DR EMBL; U53822; AAB49833.1; ALT_INIT; Genomic_DNA.
DR RefSeq; NP_001026828.1; NM_001031658.1.
DR AlphaFoldDB; P70553; -.
DR SMR; P70553; -.
DR STRING; 10116.ENSRNOP00000054282; -.
DR GlyGen; P70553; 2 sites.
DR PaxDb; P70553; -.
DR GeneID; 316519; -.
DR KEGG; rno:316519; -.
DR UCSC; RGD:1601463; rat.
DR CTD; 6556; -.
DR RGD; 1601463; Slc11a1.
DR eggNOG; KOG1291; Eukaryota.
DR InParanoid; P70553; -.
DR OrthoDB; 666470at2759; -.
DR Reactome; R-RNO-1222556; ROS and RNS production in phagocytes.
DR Reactome; R-RNO-425410; Metal ion SLC transporters.
DR Reactome; R-RNO-6798695; Neutrophil degranulation.
DR Reactome; R-RNO-6803544; Ion influx/efflux at host-pathogen interface.
DR PRO; PR:P70553; -.
DR Proteomes; UP000002494; Unplaced.
DR GO; GO:0010008; C:endosome membrane; ISO:RGD.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005770; C:late endosome; ISO:RGD.
DR GO; GO:0005764; C:lysosome; ISO:RGD.
DR GO; GO:0030670; C:phagocytic vesicle membrane; ISO:RGD.
DR GO; GO:0005886; C:plasma membrane; ISO:RGD.
DR GO; GO:0070821; C:tertiary granule membrane; ISO:RGD.
DR GO; GO:0015086; F:cadmium ion transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0005381; F:iron ion transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0005384; F:manganese ion transmembrane transporter activity; ISO:RGD.
DR GO; GO:0051139; F:metal ion:proton antiporter activity; ISO:RGD.
DR GO; GO:0042803; F:protein homodimerization activity; ISO:RGD.
DR GO; GO:0046915; F:transition metal ion transmembrane transporter activity; ISO:RGD.
DR GO; GO:0032147; P:activation of protein kinase activity; ISO:RGD.
DR GO; GO:0048002; P:antigen processing and presentation of peptide antigen; ISO:RGD.
DR GO; GO:0070574; P:cadmium ion transmembrane transport; ISO:RGD.
DR GO; GO:0006876; P:cellular cadmium ion homeostasis; ISO:RGD.
DR GO; GO:0006879; P:cellular iron ion homeostasis; ISO:RGD.
DR GO; GO:0042742; P:defense response to bacterium; ISO:RGD.
DR GO; GO:0050829; P:defense response to Gram-negative bacterium; ISO:RGD.
DR GO; GO:0042832; P:defense response to protozoan; ISO:RGD.
DR GO; GO:0006954; P:inflammatory response; ISO:RGD.
DR GO; GO:0055072; P:iron ion homeostasis; ISO:RGD.
DR GO; GO:0006826; P:iron ion transport; ISO:RGD.
DR GO; GO:1903826; P:L-arginine transmembrane transport; ISO:RGD.
DR GO; GO:0042116; P:macrophage activation; ISO:RGD.
DR GO; GO:0006828; P:manganese ion transport; ISO:RGD.
DR GO; GO:0030001; P:metal ion transport; ISO:RGD.
DR GO; GO:0045342; P:MHC class II biosynthetic process; ISO:RGD.
DR GO; GO:0048255; P:mRNA stabilization; ISO:RGD.
DR GO; GO:0060586; P:multicellular organismal iron ion homeostasis; ISO:RGD.
DR GO; GO:0001818; P:negative regulation of cytokine production; ISO:RGD.
DR GO; GO:0015707; P:nitrite transport; ISO:RGD.
DR GO; GO:0006909; P:phagocytosis; ISO:RGD.
DR GO; GO:0001819; P:positive regulation of cytokine production; ISO:RGD.
DR GO; GO:0002606; P:positive regulation of dendritic cell antigen processing and presentation; ISO:RGD.
DR GO; GO:0010628; P:positive regulation of gene expression; ISO:RGD.
DR GO; GO:0032729; P:positive regulation of interferon-gamma production; ISO:RGD.
DR GO; GO:0050766; P:positive regulation of phagocytosis; ISO:RGD.
DR GO; GO:0002827; P:positive regulation of T-helper 1 type immune response; ISO:RGD.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISO:RGD.
DR GO; GO:0045730; P:respiratory burst; ISO:RGD.
DR GO; GO:0009617; P:response to bacterium; ISO:RGD.
DR GO; GO:0034341; P:response to interferon-gamma; ISO:RGD.
DR GO; GO:0032496; P:response to lipopolysaccharide; ISO:RGD.
DR GO; GO:0002309; P:T cell proliferation involved in immune response; ISO:RGD.
DR GO; GO:0007035; P:vacuolar acidification; ISO:RGD.
DR GO; GO:0042060; P:wound healing; ISO:RGD.
DR HAMAP; MF_00221; NRAMP; 1.
DR InterPro; IPR001046; NRAMP_fam.
DR PANTHER; PTHR11706; PTHR11706; 1.
DR Pfam; PF01566; Nramp; 1.
DR PRINTS; PR00447; NATRESASSCMP.
DR TIGRFAMs; TIGR01197; nramp; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Ion transport; Iron; Iron transport; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..507
FT /note="Natural resistance-associated macrophage protein 1"
FT /id="PRO_0000212591"
FT TOPO_DOM 1..39
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 40..60
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 61..123
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 124..144
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 145..152
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 153..173
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 174..199
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 200..220
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 221..245
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 246..266
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 267..305
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 306..326
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 327..353
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 354..374
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 375..391
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 392..412
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 413..422
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 423..443
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 444..451
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 452..472
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 473..507
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 1..36
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 280
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 294
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 404
FT /note="T -> S (in Ref. 2; AAB49833)"
FT /evidence="ECO:0000305"
FT CONFLICT 409
FT /note="P -> S (in Ref. 2; AAB49833)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 507 AA; 55922 MW; 0A052B5EF53D1C64 CRC64;
MIRDKNPQRV NRPSYGSISS LPSPAPQPEP SRNTYLSEKI PIPSTEQLLW VLLWATVLGL
LCQRLAARLG VVTGKDLGEI CHLYYPKVPR ILLWLTIELA IVGSDMQEVI GTAISFNLLS
AGRIPLWGGV LITIVDTFFF LFLDNYGLRK LEAFFGFLVT IMALTFGYEY VVARPSQGAL
LKGLFLPSCP GCGQPELLQA VGIVGAIIMP HNIYLHSALV KSREVDRTRR GDVREANMYF
LTEATIALFV SFIINLFVMA VFGQAFYQQT NEEAFNICAN SSLHNYAKIF PRDNNTVSVD
IYQGGVILGC LFGPAALYIW AVGLLAAGQS STMTGTYAGQ FVMEGFLKLR WSRFARVLLT
RSCAILPTVL VAVFRDLRDL SGLNDLLNVL QSLLLPFAVL PILTFTSMPA VMQEFANGWL
SKVITSCIMA LVCAINLYFV ISYLPSLPHP AYFGLVALLA IGYLGLTAYL AWTCCIAHGA
KFLTHSSHQR FLYGLPIEEQ EGREGSG
