NRAM1_SHEEP
ID NRAM1_SHEEP Reviewed; 548 AA.
AC P49280; O19035; Q9TSE9;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2000, sequence version 2.
DT 25-MAY-2022, entry version 90.
DE RecName: Full=Natural resistance-associated macrophage protein 1;
DE Short=NRAMP 1;
DE AltName: Full=Solute carrier family 11 member 1;
GN Name=SLC11A1; Synonyms=NRAMP1;
OS Ovis aries (Sheep).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Caprinae; Ovis.
OX NCBI_TaxID=9940;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=9682440; DOI=10.1046/j.1365-2052.1998.00224.x;
RA Matthews G.D., Crawford A.M.;
RT "Cloning, sequencing and linkage mapping of the NRAMP1 gene of sheep and
RT deer.";
RL Anim. Genet. 29:1-6(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=9880672; DOI=10.1007/s003359900919;
RA Bussmann V., Lantier I., Pitel F., Patri S., Nau F., Gros P., Elsen J.M.,
RA Lantier F.;
RT "cDNA cloning, structural organization, and expression of the sheep NRAMP1
RT gene.";
RL Mamm. Genome 9:1027-1031(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 89-208.
RX PubMed=7894181; DOI=10.1007/bf00292035;
RA Pitel F., Lantier I., Riquet J., Lanneluc I., Tabet-Aoul K.,
RA Saidi-Mehtar N., Lantier F., Gellin J.;
RT "Cloning, sequencing, and localization of an ovine fragment of the NRAMP
RT gene, a candidate for the ITY/LSH/BCG gene.";
RL Mamm. Genome 5:834-835(1994).
CC -!- FUNCTION: Divalent transition metal (iron and manganese) transporter
CC involved in iron metabolism and host resistance to certain pathogens.
CC Macrophage-specific membrane transport function. Controls natural
CC resistance to infection with intracellular parasites. Pathogen
CC resistance involves sequestration of Fe(2+) and Mn(2+), cofactors of
CC both prokaryotic and eukaryotic catalases and superoxide dismutases,
CC not only to protect the macrophage against its own generation of
CC reactive oxygen species, but to deny the cations to the pathogen for
CC synthesis of its protective enzymes (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the NRAMP family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD08636.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AF005380; AAC28241.1; -; mRNA.
DR EMBL; U70255; AAD08636.1; ALT_INIT; mRNA.
DR EMBL; Z34916; CAA84395.1; -; Genomic_DNA.
DR RefSeq; NP_001009345.1; NM_001009345.1.
DR AlphaFoldDB; P49280; -.
DR SMR; P49280; -.
DR STRING; 9940.ENSOARP00000020985; -.
DR GeneID; 443365; -.
DR KEGG; oas:443365; -.
DR CTD; 6556; -.
DR eggNOG; KOG1291; Eukaryota.
DR OrthoDB; 666470at2759; -.
DR Proteomes; UP000002356; Unplaced.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0046873; F:metal ion transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0055072; P:iron ion homeostasis; IEA:UniProtKB-KW.
DR HAMAP; MF_00221; NRAMP; 1.
DR InterPro; IPR001046; NRAMP_fam.
DR PANTHER; PTHR11706; PTHR11706; 1.
DR Pfam; PF01566; Nramp; 1.
DR PRINTS; PR00447; NATRESASSCMP.
DR TIGRFAMs; TIGR01197; nramp; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Ion transport; Iron; Iron transport; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..548
FT /note="Natural resistance-associated macrophage protein 1"
FT /id="PRO_0000212592"
FT TOPO_DOM 1..55
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 56..73
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 74..82
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 83..102
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 103..139
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 140..160
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 161..164
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 165..184
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 185..193
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 194..214
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 215..237
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 238..256
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 257..284
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 285..304
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 305..346
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 347..366
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 367..397
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 398..415
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 416..426
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 427..447
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 448..463
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 464..485
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 486..493
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 494..513
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 514..548
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 1..38
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..22
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 321
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 335
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 146
FT /note="D -> Y (in Ref. 3; CAA84395)"
FT /evidence="ECO:0000305"
FT CONFLICT 407
FT /note="P -> L (in Ref. 2; AAD08636)"
FT /evidence="ECO:0000305"
FT CONFLICT 426..427
FT /note="DL -> HV (in Ref. 2; AAD08636)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 548 AA; 59411 MW; 1E6567C533E45645 CRC64;
MSGDTGTPNQ GGTRYGSISS PPSPGPQQAP PGGTYLSEKI PIPDTESGAF SLRKLWAFTG
PGFLMSIAFL DPGNIESDLQ AGAVAGFKLL WVLLWATVLG LLCQRLAARL GVVTGKDLGE
VCHLYYPKVP RTLLWLTIEL AIVGSDMQEV IGTAIAFSLL SAGRIPLWGG VLITIVDTFF
FLFLDNYGLR KLEAFFGFLI TIMALTFGYE YVVARPAQGA LLQGLFLPSC PGCGQPELLQ
AVGIVGAIIM PHNIYLHSSL VKSREVDRSR RADIREANMY FLIEATIALS VSFFINLFVM
AVFGQAFYKQ TNQAAFNICA NSSLHDYATI FPRDNLTVAV DIYQGGVILG CLFGPAALYI
WAVGLLAAGQ SSTMTGTYAG QFVMEGFLKL RWSRFARVLL TRSCAIPPTV LLAVFRDLQD
LSGLNDLLNV LQSLLLPFAV LPILTFTSMP ALMQEFANGL VSKIITSSIM VLVCAVNLYF
VISYVPSLPH PAYFSLVALL AAAYLGLTTY LVWTCLITQG ATRLAHSSHQ RFLYGLPGED
QEEGRTSG
