NRAM2_ARATH
ID NRAM2_ARATH Reviewed; 530 AA.
AC Q9C6B2; Q9XH62;
DT 11-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Metal transporter Nramp2;
DE Short=AtNramp2;
GN Name=NRAMP2; OrderedLocusNames=At1g47240; ORFNames=F8G22.4;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=10381874; DOI=10.1126/science.284.5423.2148;
RA Alonso J.M., Hirayama T., Roman G., Nourizadeh S., Ecker J.R.;
RT "EIN2, a bifunctional transducer of ethylene and stress responses in
RT Arabidopsis.";
RL Science 284:2148-2152(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
CC -!- FUNCTION: Seems to be involved in iron uptake. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the NRAMP (TC 2.A.55) family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF141204; AAD41078.1; -; mRNA.
DR EMBL; AC079677; AAG52643.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE32142.1; -; Genomic_DNA.
DR EMBL; AF410320; AAK95306.1; -; mRNA.
DR EMBL; AY102128; AAM26695.1; -; mRNA.
DR PIR; B96513; B96513.
DR RefSeq; NP_175157.1; NM_103618.3.
DR AlphaFoldDB; Q9C6B2; -.
DR SMR; Q9C6B2; -.
DR BioGRID; 26352; 18.
DR IntAct; Q9C6B2; 11.
DR STRING; 3702.AT1G47240.1; -.
DR PaxDb; Q9C6B2; -.
DR PRIDE; Q9C6B2; -.
DR ProteomicsDB; 239055; -.
DR EnsemblPlants; AT1G47240.1; AT1G47240.1; AT1G47240.
DR GeneID; 841127; -.
DR Gramene; AT1G47240.1; AT1G47240.1; AT1G47240.
DR KEGG; ath:AT1G47240; -.
DR Araport; AT1G47240; -.
DR TAIR; locus:2036751; AT1G47240.
DR eggNOG; KOG1291; Eukaryota.
DR HOGENOM; CLU_020088_5_1_1; -.
DR InParanoid; Q9C6B2; -.
DR OMA; IATFVNS; -.
DR OrthoDB; 666470at2759; -.
DR PhylomeDB; Q9C6B2; -.
DR PRO; PR:Q9C6B2; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9C6B2; baseline and differential.
DR Genevisible; Q9C6B2; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005802; C:trans-Golgi network; IDA:TAIR.
DR GO; GO:0015086; F:cadmium ion transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0005384; F:manganese ion transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0071287; P:cellular response to manganese ion; IMP:TAIR.
DR GO; GO:0055072; P:iron ion homeostasis; IEA:UniProtKB-KW.
DR GO; GO:0071421; P:manganese ion transmembrane transport; IDA:TAIR.
DR GO; GO:0006828; P:manganese ion transport; IMP:TAIR.
DR GO; GO:0051512; P:positive regulation of unidimensional cell growth; IGI:TAIR.
DR GO; GO:0048767; P:root hair elongation; IGI:TAIR.
DR GO; GO:0010015; P:root morphogenesis; IGI:TAIR.
DR HAMAP; MF_00221; NRAMP; 1.
DR InterPro; IPR001046; NRAMP_fam.
DR PANTHER; PTHR11706; PTHR11706; 1.
DR Pfam; PF01566; Nramp; 1.
DR PRINTS; PR00447; NATRESASSCMP.
DR TIGRFAMs; TIGR01197; nramp; 1.
PE 2: Evidence at transcript level;
KW Ion transport; Iron; Iron transport; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..530
FT /note="Metal transporter Nramp2"
FT /id="PRO_0000212599"
FT TRANSMEM 68..88
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 96..116
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 153..173
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 177..197
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 205..225
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 251..271
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 297..317
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 339..359
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 395..415
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 418..438
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 456..476
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 484..504
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..35
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 315
FT /note="K -> R (in Ref. 1; AAD41078)"
FT /evidence="ECO:0000305"
FT CONFLICT 405
FT /note="F -> L (in Ref. 1; AAD41078)"
FT /evidence="ECO:0000305"
FT CONFLICT 409
FT /note="E -> V (in Ref. 1; AAD41078)"
FT /evidence="ECO:0000305"
FT CONFLICT 446
FT /note="D -> V (in Ref. 1; AAD41078)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 530 AA; 58417 MW; 7CFEB7F178E2EE3D CRC64;
MENDVKENLE EEEDRLLPPP PPSQSLPSTD SESEAAFETN EKILIVDFES PDDPTTGDTP
PPFSWRKLWL FTGPGFLMSI AFLDPGNLEG DLQAGAIAGY SLLWLLMWAT AMGLLIQMLS
ARVGVATGRH LAELCRDEYP TWARYVLWSM AELALIGADI QEVIGSAIAI QILSRGFLPL
WAGVVITASD CFLFLFLENY GVRKLEAVFA VLIATMGLSF AWMFGETKPS GKELMIGILL
PRLSSKTIRQ AVGVVGCVIM PHNVFLHSAL VQSRKIDPKR KSRVQEALNY YLIESSVALF
ISFMINLFVT TVFAKGFYGT EKANNIGLVN AGQYLQEKFG GGLLPILYIW GIGLLAAGQS
STITGTYAGQ FIMGGFLNLR LKKWMRAVIT RSCAIVPTMI VAIVFNTSEA SLDVLNEWLN
VLQSVQIPFA LLPLLTLVSK EEIMGDFKIG PILQRIAWTV AALVMIINGY LLLDFFVSEV
DGFLFGVTVC VWTTAYIAFI VYLISHSNFF PSPWSSSSIE LPKRVSVSNS
